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  • 1
    Electronic Resource
    Electronic Resource
    Hydrocarbons, free fatty acids, and amino acids of sclerotia of Sclerotinia sclerotiorum (1970)
    Springer
    Archives of microbiology 75 (1970), S. 59-66 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sclerotia of Sclerotinia sclerotiorum (Lib.) D By. were obtained from commercial pea-and bean-cleaning operations or grown on potato-dextrose agar and synthetic glucose-and sucrose-salts agar media. The crude fat (ether extract) content of sclerotia varied from 0.8 to 1.5%. Extraction and fractionation of the lipids followed by gas chromatographic analysis showed that sclerotia from pea cleanings contained one predominant hydrocarbon which was absent from sclerotia produced in the laboratory. Sclerotia from natural sources and grown in the laboratory contained a similar distribution of C18 unsaturated free fatty acids, however, quantitative differences were noted. Palmitic, oleic and linoleic were the major free fatty acids of the laboratory-grown sclerotia while a high proportion of linoleic acid was also found in sclerotia from natural sources. Sclerotia were fractionated into water-soluble and water-insoluble fractions. After acid hydrolysis of the waterinsoluble fraction, both fractions were analyzed for amino acids. Twenty-one compounds, including 2 unknowns, were detected in the soluble fraction. The hydrolyzates contained 19 amino acids, including the same 2 unknowns. Two compounds tentatively identified as ornithine and γ-aminobutyric acid were found only in the water-soluble fraction. The relative amino acid composition of the water-insoluble fraction of sclerotia from various sources was fairly constant but the arginine content decreased on the synthetic media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412093
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of dimethyl sulphoxide on growth, sporulation and spore germination ofRhizopus arrhizus Fischer (1972)
    Springer
    Archives of microbiology 82 (1972), S. 184-188 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Influences of various levels of dimethyl sulphoxide (DMSO) ranging from 0.04–1.28 M on growth, sporulation and spore germination ofRhizopus arrhizus in liquid and semi-solid media were investigated. Light enhanced the inhibitory effect of DMSO on growth and sporulation in both media. Inhibition of spore germination was observed with direct treatment with DMSO. The inhibition was higher in spores obtained from cultures grown on the DMSO semi-solid media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01890409
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  • 3
    Electronic Resource
    Electronic Resource
    Free Amino Acid Pools and Enzymes in Teratonia and Habituated Tobaceo Tissue Cultures (1969)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 22 (1969), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The free amino acid content of habituated (normal) and teratoma (abnormal) tobacco tissue cultured on white's medium were compared. Significant qualitative differences (twofold or more) were observed for serine, proline, alanine, leucine, phenylalanine, and lysine. There were qualitative differences in several unidentified ninhydrin sensitive compounds. One unknown which co-chromatographed with homo-serine was present in high concentrations (9.96 μmol/g dry weight) in habituated but was lacking in teratoma tissue, Isoenzymes of an esterase, acid phosphatase, and oxidase were demonstrated in both tissues. The distinct differences in the isoenzyme pattern of acid phosphatase suggests a differential regulatory capacity of certain phosphomonoester intermediates and P1. The invertase activity was higher in habituated tissue, implying a greater capacity to utilize the sole carbon source, socrose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1969.tb09116.x
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  • 4
    Electronic Resource
    Electronic Resource
    The Influence of Salinity and Temperature on Seed Germination in Salicornia bigelovii (1971)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 24 (1971), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Seeds of Salicornia bigelovii were germinated at 4.4°C, 15.5°C, and 26.6°C in saline solution containing from 0% to 8.08% sea salt. At 4.4°C, germination was delayed until the 26th day, but the final germination per cent was high in all salinities. At 15.5°C, germination was delayed until the 19th day, and the germination per cent was higher in the higher salinities. At 26.6°C, the germination began within one day and the germination per cent was higher at the lower salinities. With the exception of 26.6°C data, the maximum germination occurred at a sea salt concentration at 4.04 % which is very close to the salinity of the sea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1971.tb06719.x
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  • 5
    Electronic Resource
    Electronic Resource
    Ethylene and ethane production in response to salinity stress (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 9 (1986), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Ethylene and ethane production in mung bean hypocotyl sections were evaluated as possible indicators of stress due to contact with four salts that are common in natural sites. Ethylene production decreased with increasing concentrations of applied NaCl and KCl. When CaCl2 was applied, the ethylene evolution was greater. However, when MgCl2 was applied, ethylene evolution remained high then decreased and at higher salt concentrations again showed an increase. NaCl (up to 0.1 kmol m−1) and KCl (up to 0.5 kmol m−3) caused a concentration-dependent increase in ethane production. The ethane production with CaCl2 was the lowest among the salts tested and only a minute increase was noticed with the increase of concentration from 0.01 to 1 kmol m−3. Ethane production showed a distinct maximum at 0.2 kmol m−3 MgCl2. The introduction of 0.01 kmol m−3 CaCl2, as well as anaerobic conditions obtained by purging vials with N2, eliminated that high ethane production. Respiratory activity of the mung bean hypocotyl sections in MgCl2 concentrations from 0 to 0.5 kmol m−3 was correlated with ethane but not with ethylene production. The ethane/ethylene ratio showed three patterns for the four salts tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1986.tb02099.x
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructure of dormant and germinated sporangiospores ofRhizopus arrhizus (1973)
    Springer
    Protoplasma 77 (1973), S. 15-33 
    Details
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ultrastructural investigations of dormant and germinated sporangiospores ofRhizopus arrhizus were conducted by the use of thin sectioning and freeze-etching procedures. Dormant spores contained deep furrows and prominent ridges with swellings along the sides of the ridges. The furrows and ridges almost disappeared as spores swelled during germination. The plasma membranes contained protuberances or depressions (approximately 50 nm diam) depending upon the nature of the fracture. Mitochondria in dormant spores were spherical and contained few cristae compared to mitochondria of germinated spores which were larger, more diverse in shape and contained abundant cristae. Treatments of spores with 20–25% glycerol prior to freeze-etching or thin sectioning resulted in the production of artifacts between the cell wall and the plasma membrane. Vesicles were rarely observed at the apices of young germ tubes, but were present in abundance in growing hyphal tips. Surface views of young germ tubes revealed the presence of microfibrils.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287290
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  • 7
    Electronic Resource
    Electronic Resource
    NaCl-salinity-dependent conversion of ACC to ethylene in the halophyte, Allenrolfea occidentalis (1986)
    Springer
    Naturwissenschaften 73 (1986), S. 274-275 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00367785
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  • 8
    Electronic Resource
    Electronic Resource
    Is dimethylsulfonium propionate an osmoprotectant of terrestrial glycophytes? (1989)
    Springer
    Naturwissenschaften 76 (1989), S. 473-475 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00366227
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  • 9
    Electronic Resource
    Electronic Resource
    Physiological studies on Phymatotrichum omnivorum VI. Lipid composition of mycelium and sclerotia (1974)
    Springer
    Mycopathologia 52 (1974), S. 261-266 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The lipid composition of the mycelium and sclerotia ofPhymatotrichum omnivorum was compared. The lipids of the mycelium contained 47.9 % polar lipids as compared to 21.4 % in the sclerotia. Sterols represented 10 % of the lipids in sclerotia as contrasted to 3.6 % of the mycelium. More monoglycerides (17.5 %) were detected in the sclerotia as compared to the mycelium (1.6 %). Fatty acid analysis indicated that linoleic acid was the predominant fatty acid in the total fatty acids fraction in both the mycelium and the sclerotia. Palmitic acid was the major free fatty acid in the mycelium, whereas myristic acid was the predominant free fatty acid in the sclerotia. In the fatty acids of the diglycerides of sclerotia, palmitic acid represented 71 % of that fraction, as compared to 6.6 % of the fatty acids of the diglycerides in the mycelium. The major fatty acid in the diglycerides of the mycelium was oleic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02198752
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  • 10
    Electronic Resource
    Electronic Resource
    Physiological studies on Phymatotrichum omnivorum X. Influence of nitrogen sources on free and bound amino acid pools (1979)
    Springer
    Mycopathologia 67 (1979), S. 95-100 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The effect of nitrogen source on the free and bound amino acids of mycelium of Phymatotrichum omnivorum (Shear) Dugg was investigated. The largest free amino acid pool was present in the natural medium and the smallest in the synthetic medium. Phymatotrichum omnivorum was able to utilize different nitrogen sources with the best growth occurring with NH4NO3. The ratio of glycine to alanine and aspartic to glutamic was around 0.25 in the free amino acid pool and around 1 in the bound amino acid pool. The free pool of glutamic acid ranged from 5.6 % to 27.2 % depending upon the nitrogen source in the media. The free pool of alanine ranged from 35.7 % to 17.2 % in relation to the nitrogen source. Most other amino acid ratios did not vary significantly between the free amino acids and the bound amino acids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00440679
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