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Notes: The unsteady streamlined motion of a constant property fluid in the unobstructed space between a pair of disks corotating at angular velocity Ω in a fixed cylindrical enclosure is investigated numerically. Two-dimensional (axisymmetric) and three-dimensional calculations are performed using a second-order accurate time-explicit algorithm. The flow configuration corresponds to that investigated experimentally by Schuler et al. [Phys. Fluids A 2, 1760 (1990)]. The steady flow solutions are characterized by a symmetrical pair of counter-rotating toroidal vortices in the cross-stream (r-z) plane. This secondary motion is driven by the radial imbalance between the outward-directed centrifugal force and the inward-directed pressure gradient force. Axisymmetric calculations predict a flow that is steady for Re〈22 200, where Re is the Reynolds number based on the disk radius, the tip speed of the disks, and the kinematic viscosity of the fluid. Above this value the motion is unsteady periodic and, while the features of the cross-stream flow pattern are broadly preserved, the symmetry of the motion about the midplane is broken by alternating periodic crossings of the toroidal vortices.This instability is maintained through an interaction that arises between outward-directed fluid in the disk Ekman layers and inward-directed fluid in the return core flow. Three-dimensional calculations at Re=22 200 and 44 400 show that the toroidal vortices acquire a time-varying sinuous shape in the circumferential direction. These calculations reveal circumferentially periodic reversals of the axial velocity component in the cross-stream plane, including the detached shear layer separating the region of motion in solid-body rotation near the hub from the potential core, in agreement with the flow visualization observations of Humphrey and Gor [Phys. Fluids A 5, 2438 (1993)]. The wavelength of this oscillation is shown to be twice that of the circumferential velocity component which is responsible for the nodal distribution of axial vorticity. When plotted on the interdisk midplane, the axial component of vorticity manifests itself as an even integer number, 2n (n=1,2,...), of circumferentially periodic foci. Experiments show that the number of foci decreases in a stepwise manner with increasing Reynolds number. For the conditions of this study, the calculated dimensionless angular velocity of the foci, ΩF/Ω, ranges from 0.55 at Re=22 200 to 0.44 at Re=44 400. These values are close to the present experimental estimate ΩF/Ω=0.5. © 1995 American Institute of Physics.

Notes: Measurements of highly accelerated turbulent boundary layers with and without mean cross-flow are presented and compared. The accelerated mean longitudinal velocity profiles suggest that the Reynolds shear stress, u'v', is greater in the two-dimensional case than in the three-dimensional case. The measured normal longitudinal Reynolds stress, u'u', is greater in the three-dimensional accelerated boundary layer case. The measurements indicate that the Reynolds stress tensor components decouple in the three-dimensional case; hence, the three-dimensionality alters the characteristic of nondecoupling observed in highly accelerated two-dimensional boundary layers. © 1995 American Institute of Physics.

Notes: A typical experimental vortex generator was perturbed by inclining the exit orifice. Instantaneous velocity fields were measured with particle image velocimetry at a Reynolds number, Γ0/ν, of 2800, which falls in the laminar regime for the axisymmetric case. Despite the nearly uniform velocity of the axisymmetric piston, the velocity exiting the cylinder is spatially and temporally non-uniform. Specifically, the exit velocity and the entrainment are larger on the short cylinder side. This fluid motion leads to an initial vortex roll-up with maximum and minimum circulation at the shortest and longest cylinder locations, respectively. A highly complex vortex structure forms, consisting of a primary vortex ring with varying circulation and branched vortex tubes that initially extend from the primary ring upstream toward the cylinder. The variation of the circulation in the primary ring and the strength of the branched vortex tubes increase with incline angle. The branched vortex tubes induce a strong cross-stream sweep of fluid toward the long cylinder side. The branched tubes convect across the cylinder exit with the sweep, break free of the cylinder, and pass through the primary ring. Beyond this time, the vortex structure consists of two closed-loop branches connected on the short cylinder side. As the flow progresses, the center of momentum moves toward the short cylinder side. As the cylinder incline angle is increased, the migration away from the centerline increases, and the flow becomes increasingly disorganized. The propagation speed and penetration distance are reduced because of the loss of coherent circulation. Qualitatively similar velocity fields and flow visualization photographs are presented for a larger (nominally turbulent) Reynolds number of 23000.© 1998 American Institute of Physics.

Notes: Experimental tests were performed on round jets exiting inclined nozzles at a Reynolds number of 9000. Both natural jets and jets forced with single frequencies corresponding to StD=0.25, 0.5, 0.75, and 1.0 were examined. In the natural case, the nozzle incline caused a mild increase in the radial spreading in the plane of azimuthal symmetry. The forcing amplified the asymmetric radial spreading by altering the vortex structure. In general, the inclined vortex rings rolled up at an angle slightly smaller than the nozzle incline angle. As the rings moved downstream, they migrated away from the jet centerline and their incline angle increased. Vortex rings generated at StD=0.5 did not pair because that Strouhal number was near the "preferred" mode. For nozzles with slight inclines, forcing at larger Strouhal numbers led to pairing near x/D=2 in order to achieve the "preferred" mode. For nozzles with larger inclines, the vortex cores broke down before pairing could occur. Forcing at a lower Strouhal number (StD=0.25) yielded ring formation at StD=0.5 and subsequent pairing. Increasing the incline angle moved the pairing location closer to the nozzle lip. Also, the pairing process was found to depend on the nozzle incline angle. © 1997 American Institute of Physics.

Notes: Complementary flow visualization photographs and numerical calculations are presented for the transitional state between the laminar and turbulent flow regimes in a helically coiled pipe. The flow visualization covers a Reynolds number range from 3800 to 8650 (890〈De〈2030, where De is the Dean number). Estimates of the wavelength and wave speed of a traveling wave instability are made from photographs and video recordings at Re=5060 and 5480 (De=1190 and 1280). The unsteady three-dimensional finite difference approximations of the Navier–Stokes equations formulated for the toroidal coordinate system are solved numerically. The calculations are performed in a curved pipe with a radius of curvature to pipe radius ratio equal to 18.2 and Re=5480 (De=1280). These test conditions match the flow visualization and previously reported laser Doppler velocimetry measurements. The calculations reveal a complex interaction between the centrifugal force and the cross-stream velocity, hence explaining the mechanism for maintaining the traveling wave. An analogy is made with known centrifugal instabilities to explain the character of the motion observed in the inner half of the pipe along planes defined by the radial and streamwise coordinate directions. Simple considerations show that the cross-stream flow has the potential for a centrifugal instability. © 1997 American Institute of Physics.

Notes: Abstract Protein loading tests for the diagnosis of heterozygous ornithine carbamoyltransferase deficiency were performed on two occasions on an asymptomatic woman whose daughter and two infant sons died of the disease. Neither loading test produced the expected increases in urinary orotic acid excretion and studies of other pyrimidine and purine metabolites in urine and plasma did not suggest that these would provide better discrimination from non-carriers. The results probably reflect an extensive inactivation of the mutant X chromosome in liver cells and reinforce the need for caution in interpreting negative test results.

Notes: Abstract This paper reports the detection of five inherited disorders of purine and one of pyrimidine metabolism using intact red blood cells (RBCs) and compares the findings with those from RBC lysate activity. Two different phosphate levels (1 and 18 mmol L−1 Pi) were used to evaluate endogenous PP-ribose-P levels and their generation by PP-ribose-P synthetase. The importance of this dual approach is demonstrated by the following evidence: (a) Six out of eight patients with no detectable hypoxanthine-guanine phosphoribosyltransferase (HGPRT) RBC lysate activity had up to 25% of normal activity in their intact RBCs. Two Lesch-Nyhan patients showed no detectable activity in intact or lysed RBCs. (b) RBC lysates from two heterozygotes for adenosine deaminase (ADA) deficiency also showed no detectable activity, but up to 60% of normal activity using intact RBCs. (c) The existence of an aberrant enzyme in a kindred with a superactive PP-ribose-P synthetase was evident from the fact that intact RBCs failed to respond normally to phosphate activation, despite normal HGPRT and adenine phosphoribosyltransferase (APRT) RBC lysate activity. (d) Raised endogenous PP-ribose-P levels in intact RBCs were demonstrable only in purine nucleoside phosphorylase (PNP) and HGPRT deficiency; levels were normal in APRT deficiency and hereditary oroticaciduria (OPRT/ODC) deficiency. The results indicate that diagnosis from RBC lysate activity alone may be misleading. Intact RBC studies clearly provide a better indication of the functional capacity of the enzymein vivo. They also show a closer correlation with the clinical phenotype and allow further insight into the associated biochemical abnormalities in some cases.

Notes: Abstract Detailed biochemical studies have been carried out in a female heterozygote for ornithine carbamoyltransferase (OCT) deficiency. Increased levels of the pyrimidines, orotic acid, uridine and uracil, were observed in plasma as well as urine by utilizing an adaptation of high performance liquid chromatography (HPLC). Urinary clearances of these compounds were high, that of orotic acid indicating net secretion. Urinary uric acid clearance was also elevated, a finding attributed to the uricosuric effect of the orotic acid excreted concomitantly. The results in this child and her family are typical of OCT deficiency. They confirm considerable genetic heterogeneity in the biochemical as well as clinical expression in this defect.

Notes: Abstract This paper reports biochemical studies in a pregnant xanthinuric female (McKusick 27830) and compares the results with findings in three other xanthinurics studied by us, including a previously unreported female who had a nephrectomy for xanthine stones. The findings of raised levels of uric acid in plasma and urine at presentation in the third trimester of pregnancy, and the subsequent fall to almost undetectable levels 6 weeks post-partum, is regarded as evidence of the extent of fetal uric acid production and clearance by the maternal circulation.