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  • 1
    Electronic Resource
    Electronic Resource
    Application of a stable cell culture assay for the functional assessment of novel MR contrast agents (1997)
    Springer
    European radiology 7 (1997), S. 527-531 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Cell culture ; Hepatocytes ; MR receptor agents
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The purpose of this study was to apply a new cell culture assay that preserves hepatocyte orientation and differentiation for screening of MR contrast agents with hepatocyte specificity. Cultured hepatocytes were sandwiched between two layers of collagen, preserving both hepatocyte function and morphology over a prolonged period of time. Plain and rhodaminated monocrystalline iron-oxide particles (MION and MION-rh) and asialoglycoprotein receptor-specific rhodaminated asialofetuin coupled to MION (MION-ASF-rh) were prepared. Dose-dependent competition experiments of these agents were performed with D( + )-galactose to determine the specificity of galactose-mediated cell uptake. To assess the impact of cell integrity on cell uptake dose-dependent functional experiments with two hepatotoxins (ethanol and CCl4) were performed. Normal cell cultures showed significantly higher fluorescent-light emission after incubation with hepatocyte-directed ASF-MION-rh than after incubation with MION-rh. Competition experiments of ASF-MION with galactose showed a dose-dependent decrease in calibrated fluorescent-light emission. Cell cultures treated with hepatotoxins demonstrated a dose-dependent reduction in calibrated fluorescent-light emission following incubation with ASF-MION-rh. The validated assay system allows assessment not only of hepatocyte specificity, but also of hepatocyte damage. Because the assay can be applied to cells from any species (rat, pig, human), it may represent an ideal test system prior to clinical trials of new hepatocyte-directed MR contrast agents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050197
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Trapping of dextran-coated colloids in liposomes by transient binding to aminophospholipid: preparation of ferrosomes
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 1193 (1994), S. 212-218 
    Details
    ISSN: 0005-2736
    Keywords: Dextran ; Iron oxide ; Liposome ; Magnetite ; Phosphatidylethanolamine
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2736(94)90350-6
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Colloidal magnetic resonance contrast agents: effect of particle surface on biodistribution
    Amsterdam : Elsevier
    Journal of Magnetism and Magnetic Materials 122 (1993), S. 383-386 
    Details
    ISSN: 0304-8853
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0304-8853(93)91115-N
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Superparamegnetic iron oxides for MRI (1993)
    Springer
    European radiology 3 (1993), S. 198-212 
    Details
    ISSN: 1432-1084
    Keywords: Antibody ; Ferrite ; Iron oxide ; Macrophage ; Magnetic resonance imaging ; Magnetite ; Reticuloendothelial system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pharmaceutical iron oxide preparations have been used as MRI contrast agents for a variety of purposes. These agents predominantly decrease T2 relaxation times and therefore cause a decrease in signal intensity of tissues that contain the agent. After intravenous adminstration, dextran-coated iron oxides typically accumulate in phagocytic cells in liver and spleen. Clinical trials have shown that iron oxide increases lesion/liver and lesion/spleen contrast, that more lesions can be depicted than on plain MRI or CT, and that the size threshold for lesion detection decreases. Decreased uptake of iron oxides in liver has been observed in hepatitis and cirrhosis, potentially allowing the assessment of organ function. More recently a variety of novel, target-specific monocrydtalline iron oxides compounds have been used for receptor and immunospecific images. Future development of targeted MRI contrast agents is critical for organ- or tissue-specific quantitative and functional MRI.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425895
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Entwicklung und experimenteller Einsatz von rezeptorspezifischen MR-Kontrastmitteln (1996)
    Springer
    Der Radiologe 36 (1996), S. 153-163 
    Details
    ISSN: 1432-2102
    Keywords: Schlüsselwörter Rezeptoren ; Spezifische Aufnahme ; Funktion ; Key words Receptors ; Specific attachment ; Function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The authors describe the feasibility of developing receptor-specific MR contrast agents for the improved detection of pathology and assessment of organ function. Receptor specificity of MR contrast agents can be achieved by binding of receptor-specific carriers to ligands. This concept leads towards a decrease in dose and thus in toxicity. Specific attachment to parenchymal cells improves tumor-organ contrast and therefore tumor detection. Specific uptake mechanisms also enable the assessment of organ function. Future design concepts of novel MR contrast agents may consider the desired uptake in specific cells or organs (ovaries, adrenal glands, lymph nodes etc.) with subsequent synthesis of appropriate carriers.
    Notes: Zusammenfassung In der vorliegenden Arbeit wird die Entwicklung und präklinische Erprobung rezeptorspezifischer MRT-Kontrastmittel zum verbesserten Nachweis pathologischer Prozesse und der Funktionsbeurteilung am Beispiel der Leber und des Pankreas beschrieben. Durch Kopplung geeigneter Carrier an die Liganden kann eine zellspezifische Aufnahme von MR-Kontrastmitteln erreicht werden. Im Vergleich zu unspezifischen Kontrastmitteln kann demnach die Dosis und die Toxizität reduziert werden. Durch die spezifische Aufnahme wird der Tumor-Organ-Kontrast und somit auch die Nachweisbarkeit von Tumoren verbessert. Über die verbesserte morphologische Beurteilung hinaus wird eine Funktionsbeurteilung von Organen oder Zellsystemen möglich. In Zukunft sollte es möglich sein, die Kontrastmittelsynthese nach der erwünschten Aufnahme in Zellsysteme (z. B. Ovarien, Nebennieren oder Lymphknoten etc.) zu planen, indem ein Carrier für das entsprechende Aufnahmesystem eingesetzt wird.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001170050053
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    Paper (German National Licenses)
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