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Mammalian cell retention devices for stirred perfusion bioreactors (1998)

Woodside, Steven M. ; Bowen, Bruce D. ; Piret, James M.

Springer

Cytotechnology 28 (1998), S. 163-175

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ISSN: 1573-0778

Keywords: centrifuge ; cross-flow filter ; review ; sedimentation ; spin-filter ; ultrasonic cell retention

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract Within the spectrum of current applications for cell culture technologies, efficient large-scale mammalian cell production processes are typically carried out in stirred fed-batch or perfusion bioreactors. The specific aspects of each individual process that can be considered when determining the method of choice are presented. A major challenge for perfusion reactor design and operation is the reliability of the cell retention device. Current retention systems include cross-flow membrane filters, spin-filters, inclined settlers, continuous centrifuges and ultrasonic separators. The relative merits and limitations of these technologies for cell retention and their suitability for large-scale perfusion are discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008050202561

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Measurement of ultrasonic forces for particle-liquid separations (1997)

Woodside, Steven M. ; Bowen, Bruce D. ; Piret, James M.

Hoboken, NJ : Wiley-Blackwell

AIChE Journal 43 (1997), S. 1727-1736

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ISSN: 0001-1541

Keywords: Chemistry ; Chemical Engineering

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology

Notes: The magnitude and direction of the ultrasonic radiation forces that act on individual particles in a standing-wave field were determined using a microscope-based imaging system. The forces are calculated from measured particle velocities assuming that the drag force, given by Stokes' law, is exactly counterbalanced by the imposed ultrasonic forces. The axial primary radiation force was found to vary sinusoidally with axial position and to be proportional to the local acoustic energy density, as predicted by theory. The magnitude of the transverse primary force was determined by two independent methods to be about 100-fold weaker than the axial force. Separation concepts exploiting the transverse force for cell retention have been successful despite the great disparity in magnitude between the axial and transvers-force components. This may be explained by the reduced hydrodynamic forces on aggregated particles in transverse flow due to their alignment in the sound field.

Additional Material: 10 Ill.