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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Energy & fuels 2 (1988), S. 150-157 
    ISSN: 1520-5029
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Inorganic chemistry 19 (1980), S. 49-54 
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Inorganic chemistry 17 (1978), S. 326-330 
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 17 (1978), S. 3109-3116 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 98 (1976), S. 7578-7585 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0568
    Keywords: Key words Choline acetyltransferase ; Acetylcholinesterase ; Olivochochlear ; Hearing ; Central auditory system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  To better understand the development of cholinergic-like neurons within the superior olivary complex, we investigated the onset and distribution of two well-known markers of cholinergic-like neurons in hamsters: choline acetyltransferase (ChAT) and acetylcholinesterase (AChE). From embryonic day (E) 14 through postnatal day (P) 0, olivary cells immunopositive for ChAT were restricted to the rostral periolivary (RPO) area. Between P0 and P3, ChAT-positive cells are found in progressively more caudal and ventral periolivary locations. Although rostral and ventral periolivary cells exhibited an early onset of ChAT expression, stable numbers were not reached until P4. In contrast, ChAT expression within the lateral superior olive (LSO) is not visible until after P0 and higher numbers of ChAT-positive cells are obtained by P5. The AChE expression lags several days but follows roughly the same pattern of onset as for ChAT. Additionally in rostral and ventral periolivary regions as well as in the LSO, there were fewer AChE-labeled cells than ChAT-labeled cells. The observed temporal relationships in cholinergic-like expression within olivary cells suggest that different cholinergic-like populations may be defined on the basis of the onset of neurotransmitter-related enzymes: RPO cells are first, cells in ventral periolivary regions are second, and cells associated with the LSO are last. The differences observed in the onset of ChAT and AChE expression may reflect differences in the timing of target innervation as well as differences in synaptogenesis.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Abdominal imaging 19 (1994), S. 8-10 
    ISSN: 1432-0509
    Keywords: Stomach neoplasms ; Gastritis cystica polyposa, diagnosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Gastritis cystica polyposa (GCP) is an uncommon lesion that usually occurs at the gastroenterostomy site, although occasionally it can be found in an unoperated stomach. We present a rare case of GCP which presented as a large polypoid mass in the gastric fundus and contained cystic components demonstrable on preoperative computed tomography.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-1424
    Keywords: Cytoskeleton ; F-actin ; Microtubules ; Potassium transport ; cAMP ; Kidney
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The influence of microtubules and F-actin on Na+-K+-Cl− cotransport was investigated in cultured cells derived from outer-medullary thick ascending limb tubules microdissected from the mouse kidney. The cultured cells contained Tamm-Horsfall protein, produced cAMP in response to dD-arginine vasopressin (dD-AVP), isoproterenol, prostaglandin E2 and forskolin (FK), and exhibited an ouabain-resistant furosemidesensitive (Or-Fs) component of 86Rb+ influx mediated by the Na+-K+-Cl− cotransporter. Both FK and dD-AVP stimulated the Or-Fs component of Rb+ influx. Neither agent altered the tubulin and cytokeratin networks nor the shape of the tight junction using a specific anti-ZO-1 antibody. In contrast, they did induce a marked redistribution of F-actin to the periphery of the cells delineating the tight junctions. Preincubation of the cells with nocodazole, to disrupt microtubules, did not alter the FK-or dD-AVP-elicited Or-Fs Rb+ influx. In contrast, phalloidin and NBD-phallicidin, which stabilize F-actin, markedly impaired the stimulation of Na+-K+-Cl− cotransport by FK or dD-AVP, without affecting the Na+-K+ ATPase pumps and the rate constant of 36Cl− and 86Rb+ efflux. These results strongly suggested that cAMP-stimulated Na+-K+-Cl− cotransport is linked to F-actin in renal TAL cells.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 159 (1997), S. 137 -147 
    ISSN: 1432-1424
    Keywords: Key words: Folate receptor — Caveolin — Caveolae — Folate transport — Glycosyl-phosphatidylinositol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. The distribution of the glycosyl-phosphatidylinositol (GPI)-anchored folate receptor (FR) in a diffuse pattern vs. functional clusters associated with caveolae has been debated. The equivocal nature of direct localization studies is due to possible experimental artifacts such as cross-linking of the protein by the antibody probes prior to fixation and alternatively the use of a disruptive fixation method. Such studies have also been complicated by the use of cells that vastly overexpress FR. In this study a monovalent probe, i.e., a biotinylated folate affinity analogue was used to covalently label FR. Cells expressing moderate levels of FR, i.e., JAR epithelial cells expressing FR-α and recombinant CHO fibroblasts expressing FR-β, were used. The affinity label and either caveolin or antigenic sites on FR were localized by electron microscopy using colloidal gold conjugated antibody probes post-embedding in the relatively permeable LR White resin. The method avoided both receptor cross-linking and early fixation steps and also enabled the use of transport permissive conditions while labeling FR at the cell surface. The results indicate that in steady-state FR is not significantly colocalized with caveolin. However, the receptor molecules occur predominantly in clusters, independent of cross-linking, providing a physical basis for the observed kinetics of receptor internalization and recycling during folate transport. Evidence is also presented to suggest that early mild fixation will disrupt the clustering of FR.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 166 (1998), S. 111-118 
    ISSN: 1432-1424
    Keywords: Key words: Cochlea — Gating current — Tetraphenylborate — Motility — Capacitance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. The outer hair cell (OHC) from the mammalian organ of Corti possesses a bell-shaped voltage-dependent capacitance function. The nonlinear capacitance reflects the activity of membrane bound voltage sensors associated with membrane motors that control OHC length. We have studied the effects of the lipophilic ions, tetraphenylborate (TPB−) and tetraphenylphosphonium (TPP+), on nonlinear capacitance and motility of isolated guinea-pig OHCs. Effects on supporting cells were also investigated. TPB− produced an increase in the peak capacitance (Cm pk ) and shifted the voltage at peak capacitance (V pkCm ) to hyperpolarized levels. Washout reversed the effects. Perfusion of 0.4 μm TPB− caused an average increase in Cm pk of 16.3 pF and V pkCm shift of 13.6 mV. TPP+, on the other hand, only shifted V pkCm in the positive direction, with no change in Cm pk . The contributions from native OHC and TPB−-induced capacitance were dissected by a double Boltzmann fitting paradigm, and by blocking native OHC capacitance. While mechanical response studies indicate little effect of TPB− on the motility of OHCs which were in normal condition or treated with salicylate or gadolinium, the voltage at maximum mechanical gain (V δ Lmax ) was shifted in correspondence with native V pkCm , and both changed in a concentration-dependent manner. Both TPB−-induced changes in Cm pk and V pkCm were affected by voltage prepulses and intracellular turgor pressure. TPB− induced a voltage-dependent capacitance in supporting cells whose characteristics were similar to those of the OHC, but no indication of mechanical responses was noted. Our results indicate that OHC mechanical responses are not simply related to quantity of nonspecific nonlinear charge moved within the membrane, but to the effects of motility voltage-sensor charge movement functionally coupled to a mechanical effector.
    Type of Medium: Electronic Resource
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