Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Chemistry of materials 7 (1995), S. 1686-1689 
    ISSN: 1520-5002
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 141 (1994), S. 277-287 
    ISSN: 1432-1424
    Keywords: Erythrocytes (red blood cells) ; Amiloride ; Magnesium ; sodium exchange ; Magnesium transport ; Sodium transport ; Hamster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Previous work showed that in hamster red cells the amiloride-sensitive (AS) Na+ influx of 0.8 mmol/liter cells/hr is not mediated by Na-H exchange as in other red cells, but depends upon intracellular Mg2+ and can be increased by 40-fold by loading cells with Mg2+ to 10 mm. The purpose of this study was to verify the connection of AS Na+ influx with Na-dependent, amiloride-sensitive Mg2+ efflux and to utilize AS Na+ influx to explore that pathway. Determination of unidirectional influx of Na+ and net loss of Mg2+ in parallel sets of cells showed that activation by extracellular [Na+] follows a simple Michaelis-Menten relationship for both processes with a K m of 105–107 mm and that activation of both processes is sigmoidally dependent upon cytoplasmic [Mg2+] with a [Mg2+]0.5 of 2.1–2.3 mm and a Hill coefficient of 1.8. Comparison of Vmax for both sets of experiments indicated a stoichiometry of 2 Na: l Mg. Amiloride inhibits Na+ influx and Mg2+ extrusion in parallel (K i = 0.3 mm). Like Mg2+ extrusion, amiloride-sensitive Na+ influx shows an absolute requirement for cytoplasmic ATP and is increased by cell swelling. Hence, amiloride-sensitive Na+ influx in hamster red cells appears to be through the Na-Mg exchange pathway. There was no amiloride-sensitive Na+ efflux in hamster red cells loaded with Na+ and incubated with high [Mg2+] in the medium with or without external Na+, nor with ATP depletion. Hence, this is not a simple Na-Mg exchange carrier.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 171 (1999), S. 117-126 
    ISSN: 1432-1424
    Keywords: Key words: Airway epithelial ion channels — Amiloride — Dichlorobenzamil — l-cis-diltiazem — Cyclic nucleotide-gated cation channels — Airway fluid absorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. Using the whole-cell patch-clamp technique, the selectivity and pharmacology of 8-Br-cGMP-stimulated currents in the human alveolar cell line A549 was compared to 8-Br-cGMP-stimulated currents in HK293 cells transfected with hαCNC1. Whole cell currents stimulated by 8-Br-cGMP in HK293 cells transfected with hαCNC1 or A549 cells are carried by inward sodium and outward potassium with nearly the same selectivity. The whole-cell inward currents that are stimulated by 8-Br-cGMP in HK293 cells transfected with hαCNC1 are inhibited by l-cis-diltiazem with an IC50 of 154 μm, by 2′,4′-dichlorobenzamil with an IC50 of 50 μm and by amiloride with an IC50 of 133 μm. The whole-cell inward currents in A549 cells that are stimulated by 8-Br-cGMP, are inhibited by l-cis-diltiazem with an IC50 of 87 μm, by 2′4′-dichlorobenzamil with an IC50 of 38 μm and by amiloride with an IC50 of 32 μm suggesting that these airway cells contain cyclic nucleotide-gated cation channels. RT-PCR data suggest that mRNA of both αCNC1 and βCNC subunits are present in A549 cells and the presence of the βCNC subunit, may as previously reported, increase the affinity of these channel blockers compared to the hαCNC1 subunit alone. The mRNA of two other isoforms of this channel, CNC2 and CNC3, are also expressed in the A549 cell line. This study documents the IC50 of externally applied channel blockers that can be used for in vitro or in vivo experiments to document sodium absorption via cyclic nucleotide-gated cation channels in airway cells.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 159 (1993), S. 392-395 
    ISSN: 1432-072X
    Keywords: Arthrobacter strain 45-8A ; Cephalosporin acylase ; 7-Aminocephalosporanic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A bacterial strain producing cephalosporin acylases was isolated from soil. The morphological and physiological properties of this strain suggest that it belongs to the genus Arthrobacter, and the isolate was therefore designated Arthrobacter strain 45-8A. Substrate specificity of the enzyme was examined. The enzyme can convert both cephalosporin C and 7β-(4-carboxylbutan-amino)cephalosporanic acid to 7-aminocephalosporanic acid. An interesting feature of the acylases is their temperature-dependent regulation. Activity of acylases was detected in strain 45-8A grown at temperature below 30 °C, but was not observed at higher temperature. Arthrobacter strain 45-8A did not exhibit β-lactamase activity, even though its resistance to cephalosporin C was very strong (〉2000 μg/ml). This is quite beneficial for its application in the manufacture of 7-aminocephalosporanic acd.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 15-17 (Feb. 2006), p. 531-536 
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: High molybdenum high strength stainless steels can contain the so-called Chi phase(Fe36Cr12Mo10). The presence of this phase, which normally occurs at grain boundaries,depletes the chromium content leading to intergranular corrosion. This may cause alloyembrittlement during long term use. The presence of such phase has proven to be highlysensitive to alloy processing parameters such as the cooling rate after a final heat treatment.The present work provides a model to quantify the effects of processing parameters aimed atcontrolling the Chi phase. The model is based on nucleation and growth classical theoriesinvolving capillarity effects for the early stages; it is applied to a range of heat treatmentconditions and compared to experimental results
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 558-559 (Oct. 2007), p. 413-418 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A typical dual-beam platform combines a focused ion beam (FIB) microscope with a fieldemission gun scanning electron microscope (FEGSEM). Using FIB-FEGSEM, it is possible tosequentially mill away 〉 ~ 50 nm sections of a material by FIB and characterize, at high resolution,the crystallographic features of each new surface by electron backscatter diffraction (EBSD). Thesuccessive images can be combined to generate 3D crystallographic maps of the microstructure. Auseful technique is described for FIB milling that allows the reliable reconstruction of 3Dmicrostructures using EBSD. This serial sectioning technique was used to investigate therecrystallization behaviour of a particle-containing nickel alloy, which revealed a number of featuresof the recrystallizing grains that are not clearly evident in 2D EBSD micrographs such as clearevidence of particle stimulated nucleation (PSN) and twin formation and growth during PSN
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 7
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 558-559 (Oct. 2007), p. 419-424 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The effect of initial microstructure (acicular ferrite (AF), polygonal ferrite (PF) and stripcast (SC)) on the recrystallization behaviour of low carbon (LC) steel was investigated. Steel stripsamples (0.05 wt.% C) of 2 mm in thickness were heat treated to produce an AF and PFmicrostructure from coarse austenite. The AF, PF and a similar chemistry SC sample manufacturedfrom a twin roll caster were cold rolled to 50, 70 and 90% reduction, and annealed for various times inthe temperature range 580-680 °C. The evolution of microstructure during recrystallization wasstudied by optical microscopy and electron backscatter diffraction (EBSD) in the SEM. The initialmicrostructure was found to have a substantial influence on the recrystallization behavior. PFrecrystallized more rapidly than AF with SC showing extremely sluggish recrystallization behaviour.The recrystallizing grains in these initial microstructures have a lognormal distribution and therecrystallized number density (grains/mm2) decreased during annealing, with the initialmicrostructures affecting the degree of this decrease in number density
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 8
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 550 (July 2007), p. 55-64 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A typical dual-beam platform combines a focussed ion beam (FIB) microscope with a fieldemission gun scanning electron microscope (FEGSEM). Using this platform, it is possible tosequentially mill off 〉 ~ 50 nm slices of a material by FIB and characterise, at high resolution, thecrystallographic features of each new surface by electron backscatter diffraction (EBSD). Thesuccessive images can be combined to generate 3-D crystallographic maps of the microstructure.This paper describes various aspects of 3-D FIB tomography in the context of understanding themicrostructural evolution of metals during deformation and annealing. The first part of the paperdescribes the influence of both metal type and milling parameters on the quality of EBSD patternsgenerated from a surface prepared by FIB milling. Single crystals of some face centred cubic metalswere examined under varying FIB milling parameters to optimise EBSD pattern quality. It was foundthat pattern quality improves with increasing atomic number with the FIB milling parameters neededto be adjusted accordingly. The second part of the paper describes a useful technique for FIB millingfor the reliable reconstruction of 3-D microstructures using EBSD. There is an initial procedureinvolving extensive milling to generate a protruding rectangular-shaped volume at the free surface.Serial sectioning is subsequently carried out on this volume. The technique was used to investigatethe recrystallization behaviour of a particle-containing nickel sample, which revealed a number offeatures of the recrystallizing grains that are not clearly evident in 2-D EBSD micrographs
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 9
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 561-565 (Oct. 2007), p. 2013-2016 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: An extra low carbon steel was cold rolled to 85% reduction and annealed at 680 °C togenerate a microstructure containing ~2 % recrystallized grains. A partly recrystallized volume wasanalyzed using 3-D FIB-EBSD tomography. The results show that nucleation and subsequent growthof recrystallizing grains is more complex processes than that revealed using 2-D metallographictechniques. In the present steel, it was found that subgrains were found to be the origin of nucleationand these grains exhibit an internal structure similar to the surrounding deformation substructure.However, a certain subgrain keeps expanding to a stage where some part or parts of the boundaryreach(es) and consume(s) a high stored energy deformation zone(s) to form (a) local dislocation freezone(s) having an orientation similar to the subgrain. After this stage, the residual dislocations in theoriginal subgrain are annihilated and nuclei enter a well-defined growth stage. The overall growth ofrecrystallization nuclei was found to be controlled by the variation in both the stored energy andorientation of the surrounding deformation substructure that results in heterogeneous growth byso-called orientation pinning
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 779 (1996), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...