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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of embryo, endosperm, cytoplasmic and maternal effects for heterosis of protein and lysine content in indica hybrid rice (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 118 (1999), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The heterosis controlled by genetic main effects and genotype × environment (GE) interaction effects for protein content and lysine content traits of indica hybrid rice, Oryza sativa L., was studied by using a genetic model for quantitative traits of triploid endosperm. The experiment was conducted over 2 years in a factorial design that included nine cytoplasmic male-sterile lines as females and five restorer lines as males. It was revealed that heterosis of protein content and lysine content were simultaneously controlled by genetic main effects and GE interaction effects. Maternal general heterosis and maternal interaction heterosis were observed. Embryo heterosis or cytoplasm heterosis for lysine content and endosperm heterosis for protein content were more important in general heterosis. Embryo interaction heterosis and cytoplasm interaction heterosis were more important for protein content, but endosperm heterosis was only important for lysine content in GE interaction heterosis. It was shown that some indica hybrid crosses had significant positive heterosis for protein content. Negative heterosis for lysine content was observed in most hybrid crosses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.1999.00418.x
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  • 2
    Electronic Resource
    Electronic Resource
    Divergence and allelomorphic relationship of a soybean virus resistance gene based on tightly linked DNA microsatellite and RFLP markers (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 64-69 
    Details
    ISSN: 1432-2242
    Keywords: Glycine max ; Potyvirus ; Disease resistance ; Germplasm ; Simple sequence repeat (SSR) ; Marker-assisted screening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The use of genetically diverse resistance sources is important in breeding for durable disease resistance. Detection and evaluation of resistance genes by conventional inheritance experiments, however, often require laborious screening and genetic testing. In the present study, a marker-assisted screening for resistance sources was initiated in soybean [Glycine max (L.) Merr] using one DNA microsatellite and two RFLP markers tightly linked to a soybean mosaic virus (SMV) resistance gene (Rsv1). The three marker loci were used to screen 67 diverse soybean cultivars, breeding lines, and plant introductions. Five variants were found at the microsatellite locus (HSP176L), and the two RFLP loci (pA186 and pK644a) near Rsv1 show a remarkably higher level of restriction polymorphism than Rsv1-independent RFLP loci. Several specific variants at the three marker loci were found to be correlated with virus resistance, among which HSP176L-2 can be detected by PCR, thus may be useful for germplasm screening. The grouping of the 67 accessions according to their multilocus marker variants agrees with the available pedigree information. When all, or most, of the cultivars within a given group with the same Rsv1-linked marker variant are resistant, their SMV resistance is most likely conferred by Rsv1. These putatively Rsv1-carrying groups contain a total of 38 SMV-resistant lines including six differential cultivars that are known to carry Rsv1. The remaining seven resistant accessions (Columbia, Holladay, Peking, Virginia, FFR-471, PI 507403, and PI 556949) do not carry resistance marker variants, and at least some of them could be sources of resistance genes independent of Rsv1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222952
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of genetic effects on nutrient quality traits in indica rice (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 1099-1102 
    Details
    ISSN: 1432-2242
    Keywords: Cytoplasmic effects ; Seed and maternal effects ; Rice nutrient quality ; Heritability ; Indica rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nine cytoplasmic male-sterile lines and five restorer lines were used in an incomplete diallel cross to analyze seed effects, cytoplasmic effects, and maternal gene effects on nutrient quality traits of indica rice (Oryza sauva L.). The results indicated that nutrient quality traits were controlled by cytoplasmic and maternal effects as well as by seed direct effects. Maternal effects for lysine content (LC), lysine index (LI), and the ratio of lysine content to protein content (RLP) were more important than seed direct effects, while protein content (PC) and protein index (PI) were mainly affected by seed direct effects. Cytoplasmic effects accounted for 2.41–20.80% of the total genetic variation and were significant for all nutrient quality traits. Additive genetic effects were much more important than dominance effects for all of the traits studied, so that selection could be applied for these traits in early generations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224055
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  • 4
    Electronic Resource
    Electronic Resource
    Analysis of genetic effects on nutrient quality traits in indica rice (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 1099-1102 
    Details
    ISSN: 1432-2242
    Keywords: Key words Cytoplasmic effects ; Seed and maternal effects ; Rice nutrient quality ; Heritability ; Indica rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Nine cytoplasmic male-sterile lines and five restorer lines were used in an incomplete diallel cross to analyze seed effects, cytoplasmic effects, and maternal gene effects on nutrient quality traits of indica rice (Oryza sativa L.). The results indicated that nutrient quality traits were controlled by cytoplasmic and maternal effects as well as by seed direct effects. Maternal effects for lysine content (LC), lysine index (LI), and the ratio of lysine content to protein content (RLP) were more important than seed direct effects, while protein content (PC) and protein index (PI) were mainly affected by seed direct effects. Cytoplasmic effects accounted for 2.41–20.80% of the total genetic variation and were significant for all nutrient quality traits. Additive genetic effects were much more important than dominance effects for all of the traits studied, so that selection could be applied for these traits in early generations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050236
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Divergence and allelomorphic relationship of a soybean virus resistance gene based on tightly linked DNA microsatellite and RFLP markers (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 64-69 
    Details
    ISSN: 1432-2242
    Keywords: Key words Glycine max  ;  Potyvirus  ;  Disease resistance  ;  Germplasm  ;  Simple sequence repeat (SSR)  ;  Marker-assisted screening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The use of genetically diverse resistance sources is important in breeding for durable disease resistance. Detection and evaluation of resistance genes by conventional inheritance experiments, however, often require laborious screening and genetic testing. In the present study, a marker-assisted screening for resistance sources was initiated in soybean [Glycine max (L.) Merr] using one DNA microsatellite and two RFLP markers tightly linked to a soybean mosaic virus (SMV) resistance gene (Rsv1). The three marker loci were used to screen 67 diverse soybean cultivars, breeding lines, and plant introductions. Five variants were found at the microsatellite locus (HSP176L), and the two RFLP loci (pA186 and pK644a) near Rsv1 show a remarkably higher level of restriction polymorphism than Rsv1-independent RFLP loci. Several specific variants at the three marker loci were found to be correlated with virus resistance, among which HSP176L-2 can be detected by PCR, thus may be useful for germplasm screening. The grouping of the 67 accessions according to their multilocus marker variants agrees with the available pedigree information. When all, or most, of the cultivars within a given group with the same Rsv1-linked marker variant are resistant, their SMV resistance is most likely conferred by Rsv1. These putatively Rsv1-carrying groups contain a total of 38 SMV-resistant lines including six differential cultivars that are known to carry Rsv1. The remaining seven resistant accessions (Columbia, Holladay, Peking, Virginia, FFR-471, PI 507403, and PI 556949) do not carry resistance marker variants, and at least some of them could be sources of resistance genes independent of Rsv1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050093
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular cloning, expression, and characterization of a thermostable glutamate racemase from a hyperthermophilic bacterium, Aquifex pyrophilus (1999)
    Springer
    Extremophiles 3 (1999), S. 175-183 
    Details
    ISSN: 1433-4909
    Keywords: Key wordsAquifex pyrophilus ; Molecular cloning ; Glutamate racemase ; Overexpression ; Thermostability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A gene encoding glutamate racemase has been cloned from Aquifex pyrophilus, a hyperthermophilic bacterium, and expressed in Escherichia coli. The A. pyrophilus glutamate racemase is composed of 254 amino acids and shows high homology with glutamate racemase from Escherichia coli, Bacillus subtilis, or Lactobacillus brevis. This racemase converts l- or d-glutamate to d- or l-glutamate, respectively, but not other amino acids such as alanine, aspartate, and glutamine. The cloned gene was expressed and the protein was purified to homogeneity. The A. pyrophilus racemase is present as a dimer but it oligomerizes as the concentration of salt is increased. The K m and kcat values of the overexpressed A. pyrophilus glutamate racemase for the racemization of l-glutamate to the d-form and the conversion of d-glutamate to the l-form were measured as 1.8 ± 0.4 mM and 0.79 ± 0.06 s−1 or 0.50 ± 0.07 mM and 0.25 ± 0.01 s−1, respectively. Complete inactivation of the racemase activity by treatment with cysteine-modifying reagents suggests that cysteine residues may be important for activity. The protein shows strong thermostability in the presence of phosphate ion, and it retains more than 50% of its activity after incubation at 85°C for 90 min.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007920050114
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  • 7
    Electronic Resource
    Electronic Resource
    Preparation of57Co standard solution and its activity measurement (1996)
    Springer
    Journal of radioanalytical and nuclear chemistry 205 (1996), S. 157-166 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract 57Co was produced with high pure nature iron irradiated by 8.5MeV deuterons. TBP-benzene extraction method and anion-exchange method were used to separate and purify it. The purified57Co was prepared into standard solution of about 30 to 50 μg Co2+/ml carrier concentration and about 0.1 mol/l HCl. The specific activity of the standard solution was measured with 4 π β (ppc)-γ coincidence counting method. The final result was 476.82(1±0.42%)Bq/mg.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02040562
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