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  • 1
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The review characterizes the submerged and solid state fermentation processes (SF and SSF) for obtaining of microbial biomass protein (MBP) on cellulose- and lignin-containing (CL) agricultural wastes (straw, wine, branches of fruit trees). There are discussed other alternatives as well. The main technological parametres are presented (protein (biomass) yield, degree of available substrate utilization, process efficiency, etc.). The use of a special stirring system of the authors' design for mycelial cultures results in an increase of CL substrate concentration in the initial nutrient medium from 2% to 8%of dry matter (DM) and in a threefold increase of the specific productivity (from 0.07 g biomass/l · h to 0.21 g biomass/l · h). The technological parametres can be increased also in the processes with substrate addition. The obtained preparations are analyzed as to their usefulness for the feeds of ruminants.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 11 (1991), S. 325-329 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The growth energetic efficiency (η) of two mycelial forms of Thielavia terrestris (pellets and diffused form) was studied by different methods. η Values determined by the pulse method are similar for the two forms, but the values determined by C balance for pellets were lower than those for diffused mycelium. These balance data prove that pellets yield more extracellular products than the diffused mycelium form, which is also confirmed by experimental data for different amounts of carbon in the culture fluid.Growth efficiency can be determined by various methods based on the principles of mass and energy balance. The estimates most frequently used are the biomass and substrate balances. However, growth efficiency determination according to oxygen balance (particularly by the pulse method) is simpler and more accurate; as it makes possible the immediate fixation of changes in the physiological condition of microorganisms and the determination of complex substrate utilization efficiency [1]. Earlier the possible use of this method for evaluating the growth efficiency of heterotrophic bacteria [2], hyphalic and yeast forms of microscopic fungi [3] was shown. The aim of the present study is the comparative investigation of the growth efficiency of two mycelial structures (hyphalic and pellets) by different methods as well as by pulse additions.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 38 (1991), S. 552-556 
    ISSN: 0006-3592
    Keywords: stirring ; turbulence ; shear effects ; lysine fermentation ; Brevibacterium flavum ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Excess turbulence caused by high-intensity stirring inhibited microbial growth and metabolism. In stirred tank bioreactors, the growth rate and lysine biosynthesis decreased in Brevibacterium flavum beyond 900 rpm, the growth rate of Trichoderma reesei on wheat straw beyond 150 rpm, and the growth rate of Saccharomyces cerevisae beyond 800 rpm. The term turbohypobiosis was introduced to describe this inhibition. Turbohypobiosis was characterized by a stress factor Fstr expressing the interaction of medium flow with microbial cells in local turbulent zones, dependent on the energy distribution of the stirring regime. Lysine synthesis was inhibited at significantly lower Fstr values than the growth of B. flavum. The main reason for the inhibition was shear effects causing decreased adenosine triphosphate (ATP) generation, lower O2 uptake, and lower specific growth rate of bacteria.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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