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Quercetin inhibits the invasion and mobility of murine melanoma B16-BL6 cells through inducing apoptosis via decreasing Bcl-2 expression (2000)

Zhang, Xianming ; Xu, Qiang ; Saiki, Ikuo

Springer

Clinical & experimental metastasis 18 (2000), S. 415-421

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ISSN: 1573-7276

Keywords: apoptosis ; Bcl-2 ; cell cycle ; invasion ; metastasis ; mobility ; melanoma B16-BL6 cells

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Quercetin has been known to have anti-tumor and anti-oxidation activities. In the present study, we have investigated its in vitro anti-metastatic activity. Quercetin inhibited the invasion and mobility of murine melanoma B16-BL6 cells in a dose-dependent manner but did not affect their adhesion to either laminin, fibronectin, or type VI collagen. Moreover, quercetin significantly inhibited the proliferation of B16-BL6 cells only in the case of time incubation longer than 48 h. Quercetin dose-dependently decreased the cell rates in S and G2–M phases of cell cycle. The effect of quercetin to cause a remarkable apoptosis of B16-BL6 cells was also demonstrated by flow cytometric assay as well as DNA fragmentation with a typical 180-bp ladder band in agarose electrophoresis and a quantitative analysis. Furthermore, quercetin markedly inhibited the expression of anti-apoptotic protein Bcl-2 but hardly influenced Bcl-XL. These results suggest that the inhibition of quercetin on invasiveness and migration of B16-BL6 cells are closely associated with the arrest of cell cycle as well as the induction of apoptosis by decreasing the Bcl-2 expression.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1010960615370

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Poly(styrene-co-acrolein) latex particles: Copolymerization and characteristics (1990)

Yan, Changhong ; Zhang, Xianming ; Sun, Zonghua ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Applied Polymer Science 40 (1990), S. 89-98

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ISSN: 0021-8995

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics

Notes: Reactive microspheres suitable for binding proteins were prepared using emulsifier-free emulsion copolymerizations of styrene (St) and acrolein (AL) with various molar ratios of monomers St and AL. A maximum polymerization rate was obtained when the molar ratio of the monomers was 1 : 1. The diameter of the latex particles increased with increase in the amount of monomer AL. The amounts of aldehyde groups on the latex particle surfaces were determined by conductometric titration. The binding capacity of the copolymer latexes with spacer molecules was also examined.

Additional Material: 7 Ill.