ISSN:
1432-136X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary 1. A radio-enzymatic technique was used to assay the catecholamines, dopamine (DA), noradrenaline (NA) and adrenaline (A) in relatively small samples of dogfish blood, obtained from an indwelling cannula. The removal of a 2 ml sample of blood has no appreciable effect on the levels of circulating catecholamines in the dogfish. Thus, the three amines could be measured before and after a particular experimental treatment by removing 2 ml of blood before and 7–8 ml of blood after the treatment. 2. In resting, normoxic dogfish at 15°C, the mean (±S.E.) levels of the three amines in the plasma were: DA, 6.0±1.2 pmol ml−1; A, 25.6±6.5 pmol ml−1; NA, 32.1±19.3 pmol ml−1. Following exposure to an inspired oxygen tension of approximately 35 mmHg for 1.5 h there was an increase in all three catecholamines to: DA, 11.3±1.4 pmol ml−1; A, 284.4±86.2 pmol ml−1; NA, 446.5±117.6 pmol ml−1. These increases are discussed in terms of the known effects of the substances on the isolated heart and branchial arteries of elasmobranchs.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00738416
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