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  • Artikel: DFG Deutsche Nationallizenzen  (6)
  • 1
    Digitale Medien
    Digitale Medien
    Genetic aspects of targeted insertion mutagenesis in yeasts (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 28 (2004), S. 0 
    Details
    ISSN: 1574-6976
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Targeted insertion mutagenesis is a main molecular tool of yeast science initially applied in Saccharomyces cerevisiae. The method was extended to fission yeast Schizosaccharomyces pombe and to “non-conventional” yeast species, which show specific properties of special interest to both basic and applied research. Consequently, the behaviour of such non-Saccharomyces yeasts is reviewed against the background of the knowledge of targeted insertion mutagenesis in S. cerevisiae. Data of homologous integration efficiencies obtained with circular, ends-in or ends-out vectors in several yeasts are compared. We follow details of targeted insertion mutagenesis in order to recognize possible rate-limiting steps. The route of the vector to the target and possible mechanisms of its integration into chromosomal genes are considered. Specific features of some yeast species are discussed. In addition, similar approaches based on homologous recombination that have been established for the mitochondrial genome of S. cerevisiae are described.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/j.femsre.2003.10.002
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  • 2
    Digitale Medien
    Digitale Medien
    Competition between n-alkane-assimilating yeasts and bacteria during colonization of sandy soil microcosms (2000)
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 126-132 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract An n-alkane-assimilating strain of Candida tropicalis was selected in sandy soil inoculated with microorganisms from contaminated sites. Competition experiments with n-alkane utilizers from different strain collections confirmed that yeasts overgrow bacteria in sandy soil. Acidification of the soil is one of the colonization factors useful for the yeasts. It can be counteracted by addition of bentonite, a clay mineral with high ion exchange capacity, but not, however, by kaolin. Strains of different yeast species showed different levels of competitiveness. Strains of Arxula adeninivorans, Candida maltosa, and Yarrowia lipolytica overgrew strains of C. tropicalis, C. shehatae or Pichia stipitis. Two strains of C. maltosa and Y. lipolytica coexisted during several serial transfers under microcosm conditions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002530000348
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  • 3
    Digitale Medien
    Digitale Medien
    The electrophoretic banding pattern of the chromosomes of Pichia stipitis and Candida shehatae (1992)
    Springer
    Current genetics 22 (1992), S. 429-431 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Pichia stipitis ; Candida shehatae ; Genetic diversity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The electrophoretic karyotype of fu1 ur strains of P. stipitis and five strains of C. shehatae were compared by means of OFAGE and TAFE techniques. Although the number of chromosomal bands was six in all cases except one, P. stipitis revealed a clearly distinct pattern in comparison to C. shehatae. Both yeasts showed remarkable chromosome length polymorphism.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00352445
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  • 4
    Digitale Medien
    Digitale Medien
    Genetic diversity of the yeast Candida utilis (1992)
    Springer
    Current genetics 22 (1992), S. 441-446 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Candida utilis ; Electrophoretic karyotype ; mtDNA restriction fragment pattern ; Genetic diversity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The electrophoretic karyotypes and some mtDNA restriction fragment patterns of 13 strains of Candida utilis and one strain of Hansenula jadinii were compared. PFGE separations revealed remarkable chromosome length polymorphisms between two groups of strains suggesting that perhaps they do not belong to the same species. However, all strains had the same or similar EcoRI, HindIII and BamHI mtDNA restriction patterns. The mtDNA genomes had an average size range of 55 kb. These results support the supposition that C. utilis is a yeast with a highly variable electrophoretic karyotype as already known for another imperfect yeast species, Candida albicans.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00326408
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  • 5
    Digitale Medien
    Digitale Medien
    Chromosomal rearrangements after protoplast fusion in the yeast Candida maltosa (1985)
    Springer
    Current genetics 9 (1985), S. 619-621 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Protoplast fusion ; Candida maltosa ; Mitotic segregation ; Chromosomal rearrangements
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Genetical analysis of fusion hybrid clones of C. maltosa L4 was performed by means of UV-induced mitotic segregation. The segregation frequencies of two linked markers could be different in several hybrid clones of one fusion combination indicating most probably position alterations of both genes on their linkage group. The differences referred to the distance between the genes, their distance to the centromere, and their sequence in relation to the centromere.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00381176
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  • 6
    Digitale Medien
    Digitale Medien
    Correlation between microbial ex situ activities of two neighbouring uncontaminated and fuel oil contaminated subsurface sites (1999)
    Springer
    Biotechnology techniques 13 (1999), S. 271-275 
    Details
    ISSN: 1573-6784
    Schlagwort(e): bioremediation ; dehydrogenase ; enumeration ; respiration ; subsurface
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract To assess the biopotential for remediation, microbiological ex situ activities were examined in a subsurface site contaminated in the past with fluctuating hydrocarbon amounts. A novel method for estimating the proportion of hydrocarbon degraders was used. There was not in all cases a correlation between the results of chemical analysis and microbiological activities. However, the level of soil respiration (between 440 and 515 μg CO2/g dry weight h−1) correlated well with hydrocarbon contamination of the subsurface.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008966517344
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