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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    International journal of cosmetic science 26 (2004), S. 0 
    ISSN: 1468-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: It has been known that high amounts of ‘heat shock proteins (HSPs)’ are produced under heat and other environmental stresses in cells. Recently, it was reported that HSP could also be accumulated in some dermal cells to protect the cellular proteins from damage caused by ultraviolet radiation. Here, the authors have focused on thermophilic cyanobacteria and the heat shock treatment. Thermophilic cyanobacteria are known to thrive under extreme environmental conditions, and produce distinctively high amounts of HSP when exposed to higher temperatures. We tested cell extract from heat-shocked cyanobacterial cells on a number of skin-related biological activities and found it significantly effective. The cyanobacterial cells, originally collected from a spa as hot as 55 °C, were cultivated in the laboratory at 50 °C and broken by a freeze-fracture method. The extract thus obtained had a superoxide dismutase (SOD)-like activity and inhibited lipid peroxide formation. We found that the extract obtained from the heat-shocked (at 63 °C) cells showed a much stronger SOD-like activity, while the inhibitory effect on lipid peroxide formation remained almost the same. Furthermore, the SOD-like activity could withstand heat up to 70 °C for as long as 1 h. Most remarkably, supplementing this heat-shocked extract strongly promoted the proliferation of human dermal fibroblast culture. Extracts from the cells grown at a regular temperature (50 °C) did not promote the proliferation of the fibroblasts. The heat-shocked extract was fractionated by ultrafiltration for further analysis. A fraction (molecular weight: 10 000–30 000) has shown the strongest activity so far. These results indicate that the heat-shocked cyanobacterial extract with its increased amounts of HSPs is a promising potent ingredient for anti-aging cosmetic materials.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 77 (2000), S. 3468-3470 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Carbon nanotubes grown on a Ni substrate and an Fe–Ni–Cr alloy substrate by plasma-enhanced chemical vapor deposition were investigated by transmission electron microscope (TEM) and energy dispersive x-ray (EDX) analysis. TEM showed that the nanotubes on both substrates have a piled-cone structure with metal particles on top which determine the diameter of the nanotubes. Their diameter ranges from 60 to 80 nm. Moreover, EDX showed that the metal particles are composed of Ni when the nanotubes are grown on Ni substrate and of Fe and Ni in the case of the Fe–Ni–Cr alloy substrate. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Journal of oral rehabilitation 30 (2003), S. 0 
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary  The finite element method was used to calculate temperature and thermal stress distribution as a result of hot/cold liquid in the mouth. This numerical study was carried out using axisymmetric finite element models and the tooth model was endodontically treated restored with cast post and cores. The two tooth models evaluated were Ti–Ti alloy and NiCr–AuPd alloy as post material and crown material with porcelain. First, temperature changes on the restored tooth as a result of hot/cold liquid in the mouth were calculated and then the thermal stress as a result of temperature changes were carried out. A fortran computer program was developed for this study. The tooth was assumed isotropic, homogenous, elastic and symmetrical. The distribution of temperature and thermal stress versus time were plotted for four critical points.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 65 (2000), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The antioxidant capabilities of whey in a Tween 20TM-stabilized salmon emulsion were investigated. Whey fractions inhibited formation of thiobarbituric acid reactive substances (TBARS) and lipid peroxides in a 10 % salmon oil emulsion in the order of whey 〉 high-molecular-weight (HMW) fraction 〉 low-molecular-weight fraction. Heating the HMW fraction exposed sulfhydryls, with optimum exposure at 80 °C. Heating the HMW fraction above 80 °C increased antioxidant activity. The HMW fraction of whey (80 °C) alone, α-tocopherol (40 ppm) alone, and their combination inhibited TBARS 59,19 and 86%, respectively, at 21 d of storage. Sulfhydryls oxidized before a-tocopherol, suggesting that sulfhydryls are the primary antioxidant. Results indicate that whey proteins could be useful antioxidants in food emulsions.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Expression of the EP4 receptor, a prostaglandin (PG)E2 receptor subtype, as well as disease suppression by the administration of a selective EP4 agonist (ONO-AE1-329) was investigated in the colorectal mucosa of rats with dextran sodium sulphate (DSS)-induced colitis. Rats were given drinking water containing 3% DSS for 2 weeks. Expression of EP4 receptor mRNA was barely detectable under normal conditions according to reverse transcription-polymerase chain reaction (RT-PCR). By 1 week after the initial administration of DSS, the receptor mRNA was strongly expressed. After ONO-AE1-329 was administered intracolonically to rats with DSS colitis for 7 consecutive days, erosion and ulceration decreased. Peripheral white blood cell (WBC) counts became less elevated. Interleukin (IL)-1β and growth-regulated gene product/cytokine-induced neutrophil chemoattractant (GRO/CINC-1) concentrations in colorectal mucosa were lower than in colitis control group (IL-1β: 12.8 ± 4.6 and 30.8 ± 6.2 µg/mg protein, P 〈 0.05; GRO/CINC-1: 15.5 ± 3.0 and 39.2 ± 5.4 µg/mg protein, P 〈 0.05), and the expression of the corresponding cytokine mRNA was strongly suppressed. IL-10 concentration was higher than in control group (14.5 ± 1.7 and 7.9 ± 1.2 µg/mg, P 〈 0.05), and the mRNA was more strongly expressed. These results suggest that the EP4 receptor is important in colonic inflammation, and that PGE2 suppresses DSS colitis at least partly via the EP4 receptor and the above cytokine changes. Intracolonic administration of selective EP4 agonist might have therapeutic applicability in inflammatory bowel disease such as ulcerative colitis.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We previously reported that intracolonic administration of enprostil, a prostaglandin-E2 (PGE2) analogue, had therapeutic effects on acute colitis induced in rodents by dextran sulfate sodium (DSS). In addition, production of growth-regulated gene product/cytokine-induced neutrophil chemoattractant-1 [GRO/CINC-1; an interleukin(IL)-8 like cytokine] was suppressed in the inflamed tissues. In the present study we used a human colon cancer cell line (HT-29) to investigate enprostil effects on the IL-8 production of intestinal epithelial cells stimulated by various stimulants. In a MTT assay, concentrations of enprostil 〉10−5M had cytotoxitic effects on HT-29 cells. Furthermore, 10−6 M enprostil suppressed IL-8 production in HT-29 cells, SW620 and CaCo2 stimulated with interleukin-1β (IL-1β) or lipopolysaccharide (LPS), but did not suppress this response when cells were stimulated with tumour necrosis factor (TNF)-α. These results suggest that enprostil affects a point in the pathway between the IL-1 receptor or LPS receptor and nuclear factor-κB(NF-κB), without affecting the pathway between the TNF receptor and NF-κB, with the latter factor being required for the IL-8 gene transcription. The therapeutic effect of exogenous enprostil on DSS colitis may involve the inhibition of IL-8 production in colonic epithelial cells stimulated by IL-1β or LPS.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Journal of oral rehabilitation 30 (2003), S. 0 
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: summary  The purpose of this study was to calculate the temperature and thermal stress distribution as a result of hot/cold liquid in the mouth. This numerical study was carried out using three-dimensional finite element models and the tooth model was crowned with Au–Pd alloy, Ni–Cr alloy and porcelain. In the first part of the study, temperature changes as a result of hot/cold liquid in the mouth were calculated. In the second part, the thermal stresses caused by temperature changes were obtained. The tooth was assumed isotropic, homogenous, elastic and unsymmetrical. The authors using fortran 77 prepared all calculation programs. The distribution of temperature and thermal stress were plotted for some critical points.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-198X
    Keywords: Key words Aquaporin-2 ; Antidiuretic hormone ; Primary polydipsia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We observed severe overhydration in an 18-month-old Japanese girl with primary polydipsia. The secretion of antidiuretic hormone (ADH) was decreased, and urinary excretion of aquaporin-2, a vasopressin-sensitive water channel protein, was suppressed under basal conditions, but the response of aquaporin-2 to ADH was essentially preserved. These findings suggest that the water channel itself was intact and that overhydration resulting from polydipsia was responsible for the decreased ADH secretion and suppression of the water channel protein.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 238-239 (Apr. 2003), p. 213-218 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 192-195 (Sept. 2000), p. 825-828 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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