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  • Artikel: DFG Deutsche Nationallizenzen  (5)
  • Digitale Medien  (5)
  • 1995-1999  (5)
Datenquelle
  • Artikel: DFG Deutsche Nationallizenzen  (5)
Materialart
  • Digitale Medien  (5)
Erscheinungszeitraum
Jahr
Schlagwörter
  • 1
    Digitale Medien
    Digitale Medien
    Chromoblastomycosis in India (1997)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of dermatology 36 (1997), S. 0 
    Details
    ISSN: 1365-4632
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Background Chromoblastomycosis is reported for the first time from the states of Jammu-Kashmir and Bihar along with two additional cases from the stales of Assam and Uttar Pradesh. The work carried out on this disease in the Indian subcontinent is reviewed. Methods Relevant mycologic investigations, including direct microscopy, histopathology, and culture, were carried out of study the four cases. Treatment was given to two patients. Results The duration of the lesions varied from 5 to 32 years. In one case the lesion was a large erythematous plaque on the buttocks extending to the backs of the thighs, and in the others they were hypertrophic lesions on the extremities. Histopathologic study of specimens carried out in three patients revealed the causative organisms. On culture, Cladosporium carrionii was isolated in one and Fonsecaea species in the other three. The lesions in one patient were surgically excised and another patient responded well of a combined regimen of amphotericin B and 5-fluorocytosine. Conclusions Although chromoblastomycosis is widely distributed in India, many patients remain undiagnosed due to a lack of proper facilities. Treatment is difficult and most patients are not able to afford the drugs prescribed for this condition.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1365-4362.1997.00008.x
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  • 2
    Digitale Medien
    Digitale Medien
    Transgenic insect-resistant maintainer line (IR68899B) for improvement of hybrid rice (1999)
    Springer
    Plant cell reports 18 (1999), S. 572-575 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Key words Transgenic maintainer line ; cryIA(b) gene ; Insect resistant ; Hybrid rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Heterosis has helped to increase rice yield in F1 hybrids by 15–20% beyond the level of inbred semidwarf varieties. For stable yield performance rice hybrids must also possess genetic resistance to biotic stresses. One of these, stem borer, reduces the expected yield of hybrid rice. The truncated synthetic cryIA(b) gene from Bacillus thuringiensis is known to be effective in controlling stem borer. The development of transformation techniques has provided the technology for incorporating this bacterial gene into the rice genome, which has not been possible by conventional breeding methods. We have introduced a new approach of using a transgenic maintainer line for developing an insect-resistant hybrid rice. An elite IRRI maintainer line (IR68899B) has been transformed with the cryIA(b) gene driven by the 35S constitutive promoter using the biolistic method. The integration and expression of the cryIA(b) gene could be demonstrated through Southern and Western blot analyses that have been carried out so far up to the T2 generations. Insect bioassay data showed an enhanced resistance to yellow stem borer in the Bt + transgenic plants. This is the first report of the development of a transgenic maintainer line for use in hybrid rice improvement.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002990050624
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  • 3
    Digitale Medien
    Digitale Medien
    Over-expression of the cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 1138-1145 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Thaumatin-like protein ; PR-5 ; Rhizoctonia solani ; Sheath blight disease ; Rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A 1.1-kb DNA fragment containing the coding region of a thaumatin-like protein (TLP-D34), a member of the PR-5 group, was cloned into the rice transformation vector pGL2, under the control of the CaMV 35S promoter. The Indica rice cultivars, ‘Chinsurah Boro II’, ‘IR72’, and ‘IR51500’ were transformed with the tlp gene construct by PEG-mediated direct gene transfer to protoplasts and by biolistic transformation using immature embryos. The presence of the chimeric gene in T0, T1, and T2 transgenic plants was detected by Southern blot analysis. The presence of the expected 23-kDa TLP in transgenic plants was confirmed by Western blot analysis and by staining with Coomassie Brilliant Blue. Bioassays of transgenic plants challenged with the sheath blight pathogen, Rhizoctonia solani, indicated that over-expression of TLP resulted in enhanced resistance compared to control plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051178
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  • 4
    Digitale Medien
    Digitale Medien
    5a,6,9,9a-Tetrahydro-4-hydroxy-6,9-methano-5H-fluoreno[2,3-b]furan-5-one and 5a,6,9,9a-tetrahydro-6,9-methano-4-methoxy-5H-fluoreno[2,3-b]furan-5-ol (1999)
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 55 (1999), S. 774-776 
    Details
    ISSN: 1600-5759
    Quelle: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Thema: Chemie und Pharmazie , Geologie und Paläontologie , Physik
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1107/S0108270199001596
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  • 5
    Digitale Medien
    Digitale Medien
    Constitutive and tissue-specific differential expression of the cryIA(b) gene in transgenic rice plants conferring resistance to rice insect pest (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 20-30 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words PEPCP ; Pith promoter ; cryIA (b) ; Rice ; YSB
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The truncated chimeric Bt gene, cryIA(b) of Bacillus thuringiensis, driven by two constitutive promoters, 35S from CaMV and Actin-1 from rice, and two tissue-specific promoters, pith tissue and pepcarboxylase (PEPC) for green tissue from maize, was introduced into several varieties of rice (indica and japonica) by microprojectile bombardment and protoplast systems. A total of 1800 putative transgenic Bt rice plants could be produced. Southern analysis revealed that more than 100 independently transformed plants could be confirmed for integration of the cryIA(b) gene. High levels of CryIA(b) proteins were obtained in the green tissue (leaves and stem) of many plants using the PEPC promoter. There was little difference in Bt protein level in leaves and stems from transgenic plants with the 35 S or Actin-1 promoter. Out of 800 Southern-positive plants that were bioassayed, 81 transgenic plants showed 100% mortality of insect larvae of the yellow stem borer (Scirpophaga incertulas). The transgene, cryIA(b), driven by different promoters showed a wide range of expression (low to high) of Bt proteins stably inherited in a number of rice varieties with enhanced yellow stem borer resistance. This first report of transgenic indica Bt rice plants with the PEPC or pith promoter either alone or in combination should provide a better strategy for providing rice plants with protection against insect pest resistance, minimizing the expression of the CryIA(b) protein in seeds and other tissues.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050862
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