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  • 1
    Digitale Medien
    Digitale Medien
    Glycation of rat sciatic nerve tubulin in experimental diabetes mellitus (1991)
    Springer
    Diabetologia 34 (1991), S. 387-389 
    Details
    ISSN: 1432-0428
    Schlagwort(e): Diabetes ; glycation ; tubulin ; microtubule ; sciatic nerve ; brain ; axonal transport
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Diabetic neuropathy is associated with some early defects of axonal transport in experimental animals. Axonal transport is dependent on intact microtubules, and unsubstituted lysine residues of tubulin are essential for microtubule polymerization. As lysine residues are the major target for the non-enzymatic attachment of glucose, the effect of diabetes on the extent of glycation of tubulin was investigated. There was a more than four-fold increase in the extent of glycation of tubulin in the sciatic nerve of rats with streptozotocin-induced diabetes of 2 weeks duration compared with control rats. In contrast, no such increase in glycation was observed in brain microtubule protein from diabetic rats at that stage of diabetes. Incubation of brain microtubule protein with glucose prior to in vitro polymerization showed that the early stages of glycation were not associated with inhibition of microtubule assembly. The observed glycation of peripheral nerve tubulin in early experimental diabetes may nevertheless contribute to axonal transport abnormalities through an as yet undetermined impairment of microtubule function.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00403175
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Non-destructive semi-quantitative determination of acetylene reduction in the field (1980)
    Springer
    Plant and soil 56 (1980), S. 335-340 
    Details
    ISSN: 1573-5036
    Schlagwort(e): Acetylene reduction ; Field method ; Nitrogen fixation ; Non-destructive ; Pisum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Direct injection of acetylene into soil around plant roots, followed by determination of ethylene/acetylene ratios in the soil atmosphere has been tested as a rapid, non-destructive method of estimating acetylene reducing activity. In pots of artificial media as well as in field soil, the ratios determined within 10 min. after injection were significantly correlated with the rates of acetylenedependent ethylene production in detached roots. The method may be useful in preliminary screening of large numbers of plant-bacteria combinations.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02205862
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