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  • Digitale Medien  (2)
  • Carboxin  (1)
  • GFP-TUA6 fusion protein  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Carboxin resistance transformation of the homobasidiomycete fungus Pleurotus ostreatus (2000)
    Springer
    Current genetics 37 (2000), S. 209-212 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Key words Basidiomycete ; Transformation ; White-rot fungi ; Carboxin ; Succinate:ubiquinone oxidoreductase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A novel selection marker gene for transformation of the white-rot basidiomycete Pleurotus ostreatus was developed by introducing a point mutation in a gene which encodes the iron-sulfur protein (Ip) subunit of succinate dehydrogenase. The mutant gene, Cbx R, encodes a modified Ip subunit with an amino-acid substitution (His239 to Leu) and confers resistance to the systemic fungicide, carboxin. The DNA sequence was integrated ectopically in the chromosome of the transformants. This is the first report of a homologous marker gene which is available for the molecular breeding of an edible mushroom.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002940050521
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Visualization of microtubules in living cells of transgenicArabidopsis thaliana (1999)
    Springer
    Protoplasma 206 (1999), S. 201-206 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Cytoskeleton ; Epidermal cell ; GFP-TUA6 fusion protein ; Microtubule ; Transgenic plants ; Tubulin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Microtubules (MTs) were visualized in living cells of several tissues in transgenicArabidopsis thaliana. The transformed Arabidopsis plant was obtained by infecting it withAgrobacterium tumefaciens carrying the GFP-TUA6 plasmid. The fluorescence of the MTs was due to the fluorescence of GFP-TUA6 that was polymerized into the MTs. The distribution patterns of the visualized MTs in the living epidermal cells of leaves was similar to that in fixed epidermal cells. The actual destruction of MTs by oryzalin was observed in a living cell. Cytochalasin B exerts no effect on the distribution pattern of MTs. The fluorescence intensity of MTs was different among cells in different tissues.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01279267
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
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