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  • 1
    Electronic Resource
    Electronic Resource
    Externally buffered enzyme electrode for determination of glucose (1984)
    s.l. : American Chemical Society
    Analytical chemistry 56 (1984), S. 1880-1884 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac00275a028
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  • 2
    Electronic Resource
    Electronic Resource
    Different Approaches to Stabilize a Recombinant Fusion Protein (1989)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 7 (1989), S. 165-168 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We have used a fusion protein between staphylococcal protein A and E. coli β–galactosidase as a model system to investigate different approaches to stabilize recombinant gene products. First, growth conditions were adapted to preferentially produce insoluble inclusion bodies. This ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0289-165
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  • 3
    Electronic Resource
    Electronic Resource
    The use of fed batch cultivation for achieving high cell densities in the production of a recombinant protein in Escherichia coli (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 14 (1994), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: The production of the fusion protein staphylococcal protein A/E. coliβ-galactosidase in Escherichiacoli was studied in batch and fed batch cultivations. Batch cultivation of a recombinant E. coli strain yielded a final cell dry weight of 16.4 g 1-1 with a final intracellular product concentration of recombinant protein corresponding to approximately 38% of the cell dry weight. Fed batch cultivation made it possible to increase the final cell dry weight to 77.0 g 1-1. The intracellular product concentration (25%) was lower as compared to batch cultivation resulting in a total concentration of recombinant protein of 19.2 g 1-1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6976.1994.tb00072.x
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  • 4
    Electronic Resource
    Electronic Resource
    Modeling of high cell density fed batch cultivation (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 14 (1994), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: Escherichia coli was grown in carbon- and energy source-limited fed batch cultures to study the effect of osmotic stress and different feed rates on the growth kinetics. An unstructured model based on the linear equation for substrate consumption provided an adequate description of the bacterial growth during the first phase of biomass production (20 h), except for cultures exposed to osmotic stress by the addition of 0.5 M NaCl. The addition of salt to the culture media had a large effect on the energetics, that could not simply be described in terms of an increased maintenance requirement. In the later phase of growth, an extensive decline in viability for all cultures was observed. Coincidentally, the specific sugar uptake rate approached a lower limit. It is concluded that the total obtainable biomass in a fed batch culture is strongly affected by the magnitudes of the substrate feed rate and the ionic strength of the culture medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6976.1994.tb00070.x
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  • 5
    Electronic Resource
    Electronic Resource
    Oxygenation of immobilized cells using hydrogen-peroxide; a model study of Gluconobacter oxydans converting glycerol to dihydroxyacetone (1982)
    Springer
    Applied microbiology and biotechnology 14 (1982), S. 64-68 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The possibility of using hydrogen-peroxide as an oxygen source in immobilized whole cell systems was investigated. The conversion of glycerol to dihydroxyacetone performed by Ca-alginate immobilized Gluconobacter oxydans was used as a model system. The influence of basic parameters, such as pH, temperature and concentration of hydrogen-peroxide, on the conversion are reported. Continuous production using a packed bed could be substantially improved by using hydrogen-peroxide as a source of oxygen. The productivity was increased almost twenty-fold in the reactor. However, hydrogen-peroxide also caused deactivation of the oxidizing of the cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00498004
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  • 6
    Electronic Resource
    Electronic Resource
    Control of immobilized, non-growing cells for continuous production of metabolites (1983)
    Springer
    Applied microbiology and biotechnology 17 (1983), S. 143-147 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A technique for maintaining constant activity during continuous production with immobilized, non-growing cells has been developed. A single stage continuous system with alginate immobilized Clostridium acetobutylicum, was mainly fed with a glucose medium that supported fermentation of acetone-butanol but did not permit microbial growth. The inactivation that occured during these conditions was prevented by pulse-wise addition of nutrients to the reactor. Using this technique the ratio of biomass to butanol was reduced to 2% (w/w) compared to 34% in a traditional batch culture. At steady state conditions butanol was the major end product with a yield coefficient of 0.20 (g/g glucose). The productivity of butanol was 16.8 g/l·day during these conditions. In a corresponding system with immobilized growing cells the ratio of biomass to butanol was 52–76% and the formation of butyric and acetic acid increased thereby reducing the yield coefficient for butanol to 0.11 (g/g). With the intermittent nutrient dosing technique constant activity from immobilized non-growing cells has been achieved for 8 weeks.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00505878
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  • 7
    Electronic Resource
    Electronic Resource
    Large scale production of chromatophores from Rhodospirillum rubrum by light-fed-batch cultivation (1985)
    Springer
    Applied microbiology and biotechnology 22 (1985), S. 83-87 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary With the purpose of large scale production of chromatophores, the photosynthetic bacterium, Rhodospirillum rubrum was cultivated in a 50 l illuminated fermenter. The influence of light intensity on the production of cell mass and the production of intracellular membrane, from which the chromatophores can be prepared, were studied. A high constant specific rate of intracellular membrane production could be obtained by successively increasing the light intensity i.e., by light fed batch cultivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00250024
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  • 8
    Electronic Resource
    Electronic Resource
    Control of glucose-fed batch cultivations of E. coli by means of an oxygen stabilized enzyme electrode (1983)
    Springer
    Applied microbiology and biotechnology 18 (1983), S. 141-147 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The design of an autoclavable oxygen stabilized electrode based on immobilized glucose oxidase and catalase, with electrolytic generation of oxygen, is presented. The electrode's behaviour was investigated in relation to parameters such as ion strength, buffer capacity, DOT in the broth and enzyme inhibition by Cl−. It was used in fed-batch cultivations of Escherichia coli for control and monitoring of glucose feeding. Use of liquid feeding data from glucose- as well as from pH control for growth characterization is outlined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00498035
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  • 9
    Electronic Resource
    Electronic Resource
    Role of proteases in fermentation of the fused protein staphylococcal protein A-human insulin-like growth factor I in Staphylococcus aureus (1988)
    Springer
    Applied microbiology and biotechnology 28 (1988), S. 258-262 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Fermentation of the fused protein staphylococcal protein A-human insulin-like growth factor I (SpA-IGF-I) was investigated. The fused protein was expressed in Staphylococcus aureus and secreted to the culture medium. The production of SpA-IGF-I was growth related and started after a short lag phase. Examination of the product quality by SDS-PAGE after IgG affinity purification showed that the product was partially degraded. Exoproteases appeared later than SpA-IGF-I and were responsible for some, but probably not all, of the degradation of the product. It was possible to influence the exoprotease activity and thereby the product degradation by varying the cultivation conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00250451
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  • 10
    Electronic Resource
    Electronic Resource
    Influence of oxygen starvation on the respiratory capacity of Penicillium chrysogenum (1985)
    Springer
    Applied microbiology and biotechnology 21 (1985), S. 228-233 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The sensitivity of Penicillium chrysogenum to oxygen starvation and to azide was investigated on cells taken from different phases of a penicillin process. These treatments caused irreversible inhibition of the oxygen uptake rate during the tropophase while the idiophase cells were resistant to either treatment. The azide resistance is supposed to depend only on the dissociation of the hydrazoic acid when the process pH increases, but the shift from sensitive to resistant cells with respect to oxygen starvation was neither caused by the pH-change nor by the glucose limitation. Inactivation (I OUR, percentage) of tropophase cells followed 1st order kinetics according to ln (1-I OUR/100)-1=0.048·t, where t is given in minutes. This means that the 1st minute of oxygen starvation may reduce the metabolic capacity by 4.7%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00295127
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