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  • Electronic Resource  (13)
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  • Electronic Resource  (13)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Skeletal radiology 6 (1981), S. 131-133 
    ISSN: 1432-2161
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Skeletal radiology 8 (1982), S. 225-227 
    ISSN: 1432-2161
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 37 (1992), S. 104-108 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Glucose uptake by whole cells of Bacteroides ruminicola B14 is constitutive. Potassium concentrations between 10 and 150 mm stimulated uptake over fourfold, while sodium had little effect on uptake. The involvement of potassium in glucose uptake by B. ruminicola was supported by strong inhibition of uptake by the ionophores valinomycin, lasalocid, and monensin. The electron transport inhibitor antimycin A had little effect on uptake, but menadione and acriflavine inhibited uptake by 30 and 48%, respectively. Potent inhibitors of uptake included oxygen, p-chloromercuribenzoate, HgCl2, and o-phenanthroline. Sodium arsenate decreased uptake by 40%, suggesting that a high-energy phosphate compound and possibly a binding protein may be involved in glucose uptake. The protonophores carbonyl cyanide m-chlorophenylhydrazone and 2,4-dinitrophenol inhibited glucose uptake by 37 and 22%, respectively. Little change in uptake activity was observed at extracellular pH values between 4.0 and 8.0. Excess (10 mm) cellobiose, maltose, and sucrose inhibited glucose uptake less than 15%. High levels (0.15% w/v) of p-coumaric acid and vanillin decreased uptake by 32 and 37%, respectively, while 0.15% ferulic acid decreased uptake by 15%.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Semigroup forum 92 (2000), S. 206-210 
    ISSN: 1432-2137
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Festuca arundinacea Schreb.) and related those concentrations to cultivar, harvest time, and grazing-animal preference. `Barcel', `Kenhy', `Kentucky-31', `Missouri-96', `Mozark', `Stargrazer', and the two accessions C1 and HiMag were established in three replicates within each of three pastures. Organic acids were determined on regrowth within each plot during four seasons and two years; amino acids were determined on regrowth of four cultivars across three replicates during both spring and fall seasons in one year. Malate and citrate were extracted with boiling water and quantified by high-performance liquid chromatography (HPLC) with an organic acid column. Amino acids were hydrolyzed, separated by ion-exchange HPLC, and quantified as their ninhydrin derivatives. Both malate and citrate concentrations differed between years. During one year only, malate concentrations were higher in Kenhy (68 g kg−1 dry matter [DM], most preferred) than in Mozark (54 g kg−1 DM, least preferred). Citrate concentrations (13 g kg−1 DM) were not different among cultivars. Eighteen amino acids (including tryptophan) accounted for 75% of total N. Thus, tissue N data were used as covariates to amino acid data in the ANOVA. Kenhy contained higher concentrations of eight amino acids than did other cultivars. These differences may reflect presence of Lolium genes in Kenhy. Cattle (Bos taurus L.) grazing preference (0 = not eaten; 10 = completely eaten) was not related to malate, citrate, or amino acid concentrations among cultivars.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Current microbiology 32 (1996), S. 327-331 
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The objective of this study was to evaluate the effect of extracellular H2 on organic acid utilization by two lactate-utilizing strains of Selenomonas ruminantium (HD4, H18). Both strains were able to grow (optical density at 600 nm ≥ after 9 h) on either aspartate, fumarate, or malate in the presence of 1 atmosphere (atm) of H2. Succinate was the major end product produced in these fermentations. When cells were incubated with lactate plus 1 atm H2, growth was minimal and little lactate was fermented. The electron transport inhibitor, acriflavine, was a strong inhibitor of growth when either strain was incubated in the presence of organic acid plus H2. Compared with glucose- or lactate-grown cells, cellular carbohydrate levels were lower for both strains in cells grown on either organic acid plus H2. These results suggest that electron transport plays a role in organic acid utilization by S. ruminantium.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Growth ofSelenomonas ruminantium HD4 in medium that contained 21mm d-lactate was stimulated to varying degrees by 10mm l-malate, 10mm fumarate, and 2% (v/v)Aspergillus oryzae fermentation extract (Amaferm). Amaferm treatment caused the greatest growth stimulation. Initial uptake rates (30s) and long-term uptake rates (30 min) ofd-lactate by whole cells ofS. ruminantium were increased in the presence of 10mm l-malate. Amaferm (25 μl/ml) also stimulated long-term uptake rates ofd-lactate, whereas fumarate had no effect. Initial uptake ofd-lactate was depressed in the presence of fumarate or Amaferm. When eitherl-malate, fumarate, or Amaferm was included in thed-lactate growth medium, a homosuccinate fermentation resulted and an inverse relationship was observed between growth (protein synthesis) and succinate production. Recent research demonstrated that Amaferm containsl-malate, and this dicarboxylic acid may be involved in stimulatingd-lactate utilization byS. ruminantium.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Current microbiology 36 (1998), S. 348-352 
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The objectives of this study were to examine the effects of chlorhexidine diacetate on growth and L-lactate production by Streptococcus bovis JB1 as well as the effects of this antimicrobial compound on the mixed ruminal microorganism fermentation. Addition of 1.8 μM chlorhexidine diacetate to glucose medium resulted in a lag in growth by S. bovis JB1, and growth was completely inhibited in the presence of 3.6, 9.0, and 18 μM chlorhexidine. When 6.2 μM chlorhexidine diacetate was added to glucose medium after 2 h of incubation, glucose utilization and L-lactate production by S. bovis JB1 were reduced. Phosphoenolpyruvate-dependent phosphorylation of 14C-glucose by toluene-treated cells of S. bovis JB1 was inhibited by increasing concentrations (1.8 to 18 μM) of chlorhexidine, whereas only the 18 μM concentration reduced the membrane potential (ΔΨ). Chlorhexidine diacetate was a potent inhibitor of L-lactate and methane production from glucose fermentation by mixed ruminal microorganisms. However, because chlorhexidine also decreased acetate and propionate concentrations and increased ammonia concentrations in mixed-culture incubations, this antimicrobial compound may have limited application as a ruminant feed additive.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Current microbiology 39 (1999), S. 231-232 
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The objective of this study was to evaluate the ability of the human colonic bacterium Bacteroides ovatus V975 to ferment fenugreek fiber (Fenufibers™), psyllium husk (Metamucil®), and wheat bran (Wheat Chex). Strain V975 was incubated in basal medium that contained 0.1 g of each fiber source for 0, 24, or 48 h. Little digestion of either fiber source was detected over 48 h, and little acetate or succinate was produced. From the lack of significant fiber digestion and fermentation by B. ovatus, it seems that all three fiber sources could be used as dietary supplements to increase roughage in the human diet.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Current microbiology 36 (1998), S. 90-95 
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Bacteroides ovatus is a Gram-negative obligate anaerobe that was isolated from the human colon and is capable of utilizing xylan. The objective of this study was to evaluate the ability of B. ovatus V975 to digest maize bran, oat bran, and wheat bran as well as the isolated cell walls from each bran source. Strain V975 was incubated in basal medium that contained either 0.1 or 0.3 g of each bran or each bran cell wall for 0, 24, 48, and 72 h. Acetate and succinate were the main products detected from each fermentation; however, less of each end product was produced from the isolated cell walls than from each bran. More of the oat bran was digested (in vitro dry matter disappearance = 74.8%) during the 72 h incubation than any other bran source. While each bran contained arabinose and xylose, more glucose, galactose, and mannose were utilized by strain V975 during the 72-h incubation than either pentose sugar. Compared with each bran, the bran cell walls had lower concentrations of most sugars, and more glucose than any other sugar was utilized by strain V975. These results suggest that strain V975 preferentially utilizes glucose, galactose, and mannose in each bran, while glucose is the main sugar fermented in bran cell walls.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Current microbiology 40 (2000), S. 387-391 
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The objectives of this study were to examine the effects of growth substrate and extracellular pH on phosphoenolpyruvate-dependent glucose phosphorylation as well as to examine how maltose is phosphorylated by the ruminal bacterium Megasphaera elsdenii B159. Phosphoenolpyruvate-dependent glucose phosphorylation by toluene-treated cells was constitutive, and glucose phosphorylation was reduced by 69% at pH 5.0. When toluene-treated cells were incubated in histidine buffer, little maltose phosphorylation occurred in the absence of inorganic phosphate. However, the addition of increasing concentrations of either potassium or sodium phosphate increased maltose phosphorylation. Maximal phosphorylation activity was observed at between 25 and 50 mM of either inorganic phosphate source. Compared with the control incubations, maltose phosphorylation was increased over threefold with 25 mM of either potassium or sodium phosphate. Phosphoglucomutase activity was detected in cell extracts of M. elsdenii B159, and this enzyme had a K m of 3.2 mM for glucose-1-P and a V max of 1836 nmol of NADP+ reduced/mg of protein per min. Maltose was also hydrolyzed by an inducible maltase (K m , 1.19 mM). To our knowledge, this is the first report of a maltose phosphorylase and a maltase in M. elsdenii.
    Type of Medium: Electronic Resource
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