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  • 1
    Electronic Resource
    Electronic Resource
    Cloning of the Rhizopus niveus pyr4 gene and its use for the transformation of Rhizopus delemar (1995)
    Springer
    Current genetics 27 (1995), S. 472-478 
    Details
    ISSN: 1432-0983
    Keywords: Rhizopus niveus ; pyr4 ; Rhizopus delemar ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have cloned a pyr4 gene encoding orotidine-5′-monophosphate decarboxylase of the filamentous fungus Rhizopus niveus. The pyr4 gene of R. nivens has an open reading frame composed of 265 amino-acid residues and has two putative introns. We have also isolated a pyr4 mutant of Rhizopus delemar from 5-fluoroorotic acid-resistant mutants and transformed it with the pyr4 gene of R. niveus as a selectable marker. Introduced DNA was integrated into the chromosome in a multiple tandem array. The mitotic stability of the introduced DNA was increased by a repeated sporulation process. The expression of the Escherichia coli β-glucuronidase gene in R. delemar was successfully obtained under the control of the pgk2 gene promoter of R. niveus by co-transformation with the pyr4 gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00311218
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  • 2
    Electronic Resource
    Electronic Resource
    Updating the clinical experience in endometriosis – the Brazilian perspective (1995)
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 102 (1995), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In an open-label, multicentre, randomized, parallel group study, 164 women with endometriosis were assigned to treatment. Out of these women, 81 received danazol (600 mg daily for 8 weeks, then 400 mg for 16 weeks) and 83 were given gestrinone (2.5 mg twice a week for 24 weeks). Five weeks before the start of treatment clinical evaluation and diagnostic laparoscopy were performed during the screening visit. Drug assignment and laboratory data assessment were carried out within 3 days of the estimated onset of the menstrual cycle at baseline visit. The response to treatment was assessed during visits at weeks 2, 4, 8, 12, 16, 20 and 24; at the last visit a second laparoscopy was performed. Therapeutic efficacy was evaluated by analysis of the laparoscopic scores assessed according to the revised American Fertility Society classification. Symptomatic response was measured by clinical scores and laboratory data. In one centre, bone mineral density was also recorded. One patient in the danazol group discontinued treatment due to a cutaneous rash as a probable adverse reaction at the beginning of the study. The therapeutic efficacy of danazol and gestrinone did not differ significantly when the revised American Fertility Society scores were compared. The symptomatic response also showed no statistical difference when clinical examination scores were analysed. There was no significant difference between the drugs in laboratory data, including bone mineral density, with respect to adverse events. Analysis of clinical scores showed that danazol was superior to gestrinone with respect to acne and irregular bleeding. Based on these data, we conclude that both danazol and gestrinone are reliable in the treatment of endometriosis and offer similar results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.1995.tb09161.x
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  • 3
    Electronic Resource
    Electronic Resource
    Isotope Effect of Highly Anisotropic Hydrogen Atomic Emission from Laser Breakdown Plasma of Microparticles in Water (1995)
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 99 (1995), S. 875-878 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/j100003a007
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  • 4
    Electronic Resource
    Electronic Resource
    Enhanced photoluminescence detection of oxygen in silicon crystal by formation of a carbon–oxygen complex through carbon implantation and electron irradiation (1995)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 78 (1995), S. 4407-4410 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: In order to develop a method to detect low-level oxygen in thin silicon crystals by photoluminescence (PL), a radiative carbon–oxygen center (C center: 0.79 eV) is formed in silicon crystals through carbon introduction by ion implantation. The PL intensity of the C center of the implanted sample is significantly increased by annealing the sample at 1000 °C followed by electron irradiation. The possibility of detecting a low concentration of oxygen (below 1×1015 atoms/cm3) in a layer thinner than 1 μm thick is seen. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.359847
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  • 5
    Electronic Resource
    Electronic Resource
    Diluted rotifer, Brachionus plicatilis (Muller), extract as culture medium stimulates hatching of red sea bream, Pagrus major (Temminck & Schlegel) (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 26 (1995), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Hatching rates and hatching times (50% hatch) of red sea bream, Pagrus major (Temminck & Schlegel), eggs in culture media that were prepared to dilute rotifer Brachionus plicatilis Muller extract at 4000-, 6000-. 8000- and 10 000-fold with artificial sea water (σ15, = 24.0) were investigated. Although differences in hatching rate between the culture media and control artificial sea water were negligible, hatching time was significantly shorter in media at 4000- and 6000-fold dilutions than in media at 8000- and 10 000-fold dilutions and in the control artificial sea water, when the eggs immediately post spawning were incubated. The hatching rates of eggs at three developmental stages, immediately post-spawning, gastrula stage and heart-beat initiation stage, incubated respectively in the medium at 6000-fold dilution and the control, did not differ either, yet the hatching times at each egg stage in the medium were significantly shorter than that in the control. These results suggest that hatching enzyme secretion from the hatching glands is promoted to perceive chemical stimuli in the water-soluble fraction of rotifer extract via the embryo's chemoreceptors, from the heart-beat initiation stage to immediately before hatching, thus shortening the hatching time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2109.1995.tb00909.x
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  • 6
    Electronic Resource
    Electronic Resource
    Lake Biwa: Have sustainable development objectives been met? (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Lakes & reservoirs 1 (1995), S. 0 
    Details
    ISSN: 1440-1770
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geography
    Notes: The need to control and manage the effects of urban, industrial and agricultural development on Lake Biwa, Japan's largest lake, led to the formation of the Lake Biwa Comprehensive Development Project (LBCDP). This article describes the nature and effects of the development, how the LBCDP was conceptualized, implemented and managed, and comments on its effectiveness. Although the basic objective of the project was to promote development of the Keihanshin region by providing additional water, other important objectives included the conservation of the natural environment, the promotion of public welfare, and the restoration of water quality. To achieve objectives on a sustainable basis, extensive political, legislative, social, technological and environmental matters needed comprehensive consideration. This article considers such matters in detail.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1440-1770.1995.tb00002.x
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  • 7
    Electronic Resource
    Electronic Resource
    Histochemical study of Ca2+-ATPase activity in ischemic CA1 pyramidal neurons in the gerbil hippocampus (1995)
    Springer
    Acta neuropathologica 90 (1995), S. 448-453 
    Details
    ISSN: 1432-0533
    Keywords: Cerebral ischemia ; Cell death ; Hippocampus ; Ca2+-ATPase ; Ultracytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Although cytosolic Ca2+ accumulation plays a pivotal role in delayed neuronal death, there have been no investigations on the role of the cellular Ca2+ export system in this novel phenomenon. To clarify the function of the Ca2+-pump in delayed neuronal death, the plasma membrane Ca2+-ATPase activity of CA1 pyramidal neurons was investigated ultracytochemically in normal and ischemic gerbil hippocampus. To correlate enzyme activity with delayed neuronal death, histochemical detection was performed at various recirculation times after 5 min of ischemia produced by occlusion of the bilateral carotid arteries. At 10 min after ischemia, CA1 pyramidal neurons showed weak Ca2+-ATPase activity. Although enzyme activity had almost fully recovered 2 h after ischemia, it was reduced again 6 h after ischemia. Thereafter, Ca2+-ATPase activity on the plasma membrance of CA1 pyramidal neurons decreased progressively, losing its localization on day 3. On day 4 following ischemia, reaction products were diffusely scattered throughout the whole cell body. Our results indicate that, after once having recovered from ischemic damage, severe disturbance of the membrane Ca2+ export system proceeds from the early stage of delayed neuronal death and disturbs the re-export of accumulated cytosolic Ca2+, which might contribute to delayed neuronal death. Occult disruption of Ca2+ homeostasis seems to occur from an extremely early stage of delayed neuronal death in CA1 pyramidal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294804
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  • 8
    Electronic Resource
    Electronic Resource
    Prolonged but not acute fluoxetine administration produces its inhibitory effect on hippocampal seizures in rats (1995)
    Springer
    Psychopharmacology 118 (1995), S. 305-309 
    Details
    ISSN: 1432-2072
    Keywords: Epilepsy ; Serotonin ; Fluoxetine ; Gepirone ; Long-term treatment ; Hippocampus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study assessed the effects of acute as well as long-term administration of fluoxetine, a selective serotonin (5-HT) reuptake inhibitor with anti-depressant properties, on hippocampal (HIP) seizures elicited by electrical stimulation in rats. The fluoxetine effect on HIP seizures was also assessed following long-term treatment with gepirone, a 5-HT1A receptor agonist. Acute single administration of fluoxetine (1, 10 mg/kg; IP) was found to produce no significant effect on HIP seizure activity. Following daily IP administration of fluoxetine (10 mg/kg per day) or gepirone (10 mg/kg per day) for 21 days, animals were given a 7-day drug-free period and then challenged with an acute dose of 10 mg/kg fluoxetine. These treatment regimens resulted in a significantly increased afterdischarge threshold of HIP seizures in response to acute fluoxetine administration. The inhibitory effect of fluoxetine, however, was not present 4 weeks after long-term treatment with either fluoxetine or gepirone. The present results indicate that long-term treatment with these compounds enhances the antiepileptic effect of subsequent fluoxetine administration on the generation of HIP seizures. This effect is possibly related to the well-demonstrated evidence that fluoxetine and gepirone, on long-term treatment, facilitate net 5-HT neurotransmission through desensitization of presynaptic 5-HT autoreceptors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02245959
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  • 9
    Electronic Resource
    Electronic Resource
    Iliopsoas bursa of the rheumatoid hip joint. A case report and review of the literature (1995)
    Springer
    Clinical rheumatology 14 (1995), S. 358-364 
    Details
    ISSN: 1434-9949
    Keywords: Iliopsoas Bursa ; Rheumatoid Arthritis ; Magnetic Resonance Imaging ; Gadolinium-DTPA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Presented is the case of a 63-year-old woman, with a 30-year history of rheumatoid arthritis, whose hip was completely destroyed and accompanied with enlargement of the iliopsoas bursa. Preoperative diagnosis was confirmed by computed tomography, magnetic resonance (MR) imaging and arthrography. She was treated by resection of the iliopsoas bursa and total prosthetic replacement of the hip joint. The pathogenesis is uncertain. In the literature, enlargement of the iliopsoas bursa with rheumatoid arthritis, osteoarthritis, pigmented villonodular synovitis, and synovial chondromatosis had been reported. Nevertheless, a correct preoperative diagnosis of the enlargement of the iliopsoas bursa is very difficult. MR imaging with enhanced Gadolinium-DTPA (Gd-DTPA) is proposed as the most useful examination for preoperative diagnosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02208355
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  • 10
    Electronic Resource
    Electronic Resource
    Mechanisms of ergonovine-induced hyperconstriction of coronary artery after x-ray irradiation in pigs (1995)
    Springer
    Basic research in cardiology 90 (1995), S. 167-175 
    Details
    ISSN: 1435-1803
    Keywords: Ergonovine ; coronary hyperconstriction ; serotonin2 receptor ; endothelium-dependent relaxation ; x-ray irradiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Mechanisms of ergonovine-induced coronary hyperconstriction were examined in vivo and in vitro in miniature pigs. To provoke coronary hyperconstriction, the endothelium of a segment of a major branch of the left coronary artery was denuded in 19 Göttingen miniature pigs (4 to 6 months of age). In Group I (n=12), the denuded site of the coronary artery was selectively irradiated with 15 Gy of x-ray twice, 3 and 4 months after endothelial denudation. The remaining 7 pigs were not irradiated (Group II). The vasoconstrictive effect of intracoronary administration of ergonivine (1 to 1000 μg) was examined angiographically 3 months (just before irradiation in group I) and 5 months after denudation in the two groups. After the angiographical study, the vessels were isolated and isometric tensions were measured in an organ chamber. In the in vivo studies, ergonovineinduced vasoconstriction at the denuded and x-ray irradiated site in Group I was significantly greater than that at the control site or that at the denuded site in Group II. Pretreatments with serotonin receptor blockers (ketanserin or methysergide) significantly attenuated ergonovine-induced hyperconstriction, while an alpha-adrenergic receptor blocker (prazosin) did not (% inhibition; ketanserin 74±9%, p〈0.01, methysergide 60±10%, p〈0.01, prazosin 9±5%, NS). In the in vitro studies, ergonivine produced significantly greater tension at the denuded and x-ray irradiated site (Group I) than at the control site or at the denuded site (Group II). Ergonovine-induced endotheliumdependent relaxation was impaired at the denuded site in both groups to a similar extent. These results suggest that ergonovine-induced hyperconstriction at the denuded and x-ray irradiated coronary artery resulted mainly from the hyperreactivity of medial smooth muscle mediated by serotonin2 receptors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00789446
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