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  • 2005-2009  (2)
  • 1980-1984  (10)
  • 1
    Electronic Resource
    Electronic Resource
    Changes in Natural Killer Activities in Experimental Secondary Amyloidosis (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 19 (1984), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Natural killer (NK) cell aciiviiy in experimental murine amyloidosis was studied. In CBA/J mice, which show a high incidence of amyloidosis. NK activity was significantly decreased after 1 week of casein treatment. In C3H mice, which show a low incidence of amyloidosis, NK activity was not changed by casein treatment. Pretreatment with lipopolysaccharide in vivo enhanced the NK activities in CBA/J and C3H mice. These increases were not observed after casein treatment. The lowered NK activity of cells from CBA/J mice after casein treatment was restored to the normal range by indomethacine in vitro. Depletion of adherent cells from the spleen cells treated with casein had no effect on NK activity. Single-cell assay showed that casein treatment impaired the killing but not the binding of NK cells to target cells. After casein treatment, the splenic serum amyloid A (SAA) level gradually increased in CBA/J mice but remained low in C3H mice. NK activity was suppressed by the addition of serum obtained from CBA/J mice treated with casein but not by normal control serum. And partially purified AA protein obtained from the spleen of CBA/J mice treated with casein also suppressed NK activity in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1984.tb00962.x
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  • 2
    Electronic Resource
    Electronic Resource
    Increase in killer-specific secretory protein of 37 kDa in bronchoalveolar lavage fluid of allergen-challenged patients with atopic asthma (2005)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 35 (2005), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Atopic asthma is linked to a T-helper type 2 dominated pathogenesis, but there is increasing evidence of Th1/Tc1-mediated processes in the aetiopathology of asthma. Killer-specific secretory protein of 37 kDa (Ksp37) is expressed in cytotoxic lymphocytes, selectively in the effector subsets of CD8+- and CD4+ T lymphocytes and in CD16+/CD56dim natural killer cells and γ/δ T cells. This effector cell-specific expression of Ksp37 and its coexpression with perforin suggest that Ksp37 might be involved in processes mediated by cytotoxic cells.Objective We hypothesize that Ksp37 could indicate the involvement of cytotoxic lymphocytes in the pathogenesis of atopic asthma, and investigated Ksp37 concentration in bronchoalveolar lavage fluid (BALF) collected 10 min, 18, 42 or 162 h after segmental allergen provocation and in serum of patients with atopic asthma (n=25).Methods Ksp37 concentrations in BALF and serum were detected by ELISA. Flow cytometric analysis was used to assess numbers and cell subsets in BALF.Results Ksp37 increased significantly in BALF 10 min, 18 and 42 h, but not 162 h after allergen challenge compared with saline-challenged controls, while Ksp37 serum levels did not change significantly at all time-points. In addition, the increase in Ksp37 concentrations in BALF correlated with the corresponding numbers of lymphocytes.Conclusions We conclude that Ksp37 level increased in BALF 10 min, 18 and 42 h after allergen challenge but not in peripheral blood. Our findings suggest that segmental allergen challenge in asthma is associated with an increase in Ksp37 concentrations in BALF and an influx of potentially cytotoxic T lymphocytes into the lungs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.2005.02238.x
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  • 3
    Electronic Resource
    Electronic Resource
    The structure of the cytosine–calcium chloride (1:1) complex. The first evidence for direct binding of calcium to cytosine base (1980)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 36 (1980), S. 1793-1797 
    Details
    ISSN: 1600-5740
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S056774088000725X
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  • 4
    Electronic Resource
    Electronic Resource
    The structure of the 1-thyminylacetic acid and tyramine (1:1) complex (1980)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 36 (1980), S. 2095-2099 
    Details
    ISSN: 1600-5740
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S056774088000800X
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  • 5
    Electronic Resource
    Electronic Resource
    Increased plasma concentration of cyclic GMP in atrial fibrillation (1983)
    Springer
    Cellular and molecular life sciences 39 (1983), S. 1290-1291 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Plasma concentration of cyclic nucleotides in patients with paroxysmal atrial fibrillation was determined by ultrasensitive radioimmunoassay on the day of attack and also the next day, following recovery to sinus rhythmus. The concentration of cyclic GMP in the plasma of patients with attacks of paroxysmal atrial fibrillation was significantly higher than in those with sinus rhythmus, but no significant difference in plasma concentration of cyclic AMP was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01990375
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  • 6
    Electronic Resource
    Electronic Resource
    Temperature and Strain Rate Effects on Plastic Deformation of Titanium Alloys (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 539-543 (Mar. 2007), p. 3619-3624 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Since titanium alloys are the most promising structural materials for the high velocityvehicles, the impact tensile strength of the materials is presently investigated. Three kinds ofaging treatments on the beta-titanium alloy were performed, and the tensile deformation behaviorswere identified in the wide range of the temperature and the strain rate. The stress－strain relationsof the titanium alloy significantly depend on the temperature and the strain rate investigated.Thermally activated process concept was applied to explain the experimental results, and thestress-strain relations at high strain rates were well understood with taking account of adiabaticheating effect. It has been found that the stress-strain curves depend on the microstructures, whilethe temperature and the strain rate effects are almost independent of the different aging treatments
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/15/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.539-543.3619.pdf
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  • 7
    Electronic Resource
    Electronic Resource
    A new one-step method for the cytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase activity (1980)
    Springer
    Histochemistry and cell biology 67 (1980), S. 125-138 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A new one-step method for the light and electron microscopic localization of the ouabain-sensitive, K-dependent p-nitrophenylphosphatase (K-NPPase) activity of the Na-K-ATPase complex is introduced. The incubation medium contains p-nitrophenylphosphate (NPP) as substrate, lead citrate as the capture reagent, and dimethylsulfoxide (DMSO) as an activator. It is usable at the optimal pH of the K-NPPase, which is about pH 9.0 in the presence of 25% DMSO. The effects of fixation, lead concentration, and DMSO on the enzyme activity were studied using rat kidney as a test tissue. The fixation of tissues in a mixture of 2% paraformaldehyde and 0.5% glutaraldehyde for 60 min at 0°–4° C preserved 45% of the enzyme activity. In the absence of DMSO, lead citrate (4.0 mM) caused 82% inhibition of the enzyme activity in fixed tissue. However, the addition of DMSO (25%) caused about 3-fold activation of the remaining activity. Cytochemical demonstration of the ouabain-sensitive K-NPPase activity was successfully made by this method at both light and electron microscopic levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493231
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  • 8
    Electronic Resource
    Electronic Resource
    Ultrastructural localization of phosphatases in the testes of the domestic fowl: Acid phosphatase and thiamine pyrophosphatase (1982)
    Springer
    Histochemistry and cell biology 74 (1982), S. 157-169 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary ACPase and TPPase activity has been examined in the germinal epithelium of the testes in the domestic fowl. ACPase activity in spermatogonia and spermatocytes was confined to the Golgi complex. In spermatids ACPase activity was seen in the endoplasmic reticulum and nuclear envelope in the phase I and especially in the phase II (the elongating phase). This activity gradually decreased during the next phase III, and had disappeared in the final phase IV. The membrane body showed ACPase reaction in the small peripheral vacuoles and cisternal structures surrounding large central vacuoles. ACPase was also present in vesicles surrounding the developing tail. Late spermatids showed an abundance of autophagic vacuoles which had a complex array of ACPase positive delimiting membranes. In Sertoli cells ACPase activity was predominant in the lysosomes. TPPase activity was seen in the cisternae of the Golgi complex in spermatogonia and spermatocytes. In spermatids activity was present in the endoplasmic reticulum during the phase II, but it is lost in later stages. The smaller vacuoles and cisternal structures in the membrane body also showed reaction products. According to the present results it is thought likely that the smaller vacuoles and cisternal structures of the membrane body are of endoplasmic reticulum origin. The autophagic vacuoles in spermatids and the lysosomes of Sertoli cells are considered responsible for the degradation of residual bodies cast off by spermatids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495827
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  • 9
    Electronic Resource
    Electronic Resource
    Energy-dependent lysosomal wrapping mechanism (LWM) during autophagolysosome formation (1982)
    Springer
    Histochemistry and cell biology 76 (1982), S. 479-488 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The relation of lysosomes to autophagy, in particular the lysosomal wrapping mechanism (LWM) is investigated ultracytochemically from the viewpoint of demonstrations of energy dependency of the LWM. An induction of the LWM was made in mouse subcutaneous histiocytes by subcutaneous administration of ovalbumin. Prior administration of the inhibitor of glycolysis (2-deoxyglucose) alone and of the oxidative phospholylation (sodium azide or 2,4-dinitrophenol) alone did not prevent the occurence of the LWM following ovalbumin administration, but a prior administration of a mixture of 2-deoxyglucose and 2,4-dinitrophenol or 2-deoxyglucose and sodium azide prevented the occurence of the LWM. These results suggest that in order for the LWM to take place ATP is required as an energy source.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00489903
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  • 10
    Electronic Resource
    Electronic Resource
    Effects of NaOH-PIPES buffer used in aldehyde fixative on alkaline phosphatase activity in rat hepatocytes (1983)
    Springer
    Histochemistry and cell biology 77 (1983), S. 339-351 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Effects of NaOH-PIPES buffer used as a vehicle for aldehyde fixative on alkaline phosphatase (ALPase) activity demonstrated cyto- and biochemically were compared with those of routinely used cacodylate buffer. The reaction products showing ALPase activity demonstrated ultracytochemically were confined to the bile canalicular membranes when cacodylate buffer (0.1 M) was used. However, when PIPES1 buffer (0.03 M or 0.1 M) was used, the activity was observed on whole membranes of hepatocytes. The activities of the sinusoidal, lateral and bile canalicular membranes were completely suppressed by an addition of 2.5 mM levamisole. Moreover, the same results were obtained when HEPES2 or low concentration of cacodylate buffer (0.01 M) was used. Biochemical estimation revealed that much higher activity was retained when PIPES or HEPES buffer was used as compared with that when cacodylate buffer was used. Maximum preservation of ALPase activity was obtained when PIPES buffer was used. Cacodylate buffer showed an inhibitory effect on the hepatic ALPase activity in proportion to the buffer concentration. In conclusion, PIPES buffer preserves the alkaline phosphatase activity much better and is a better vehicle for the aldehyde fixatives in alkaline phosphatase cytochemistry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490897
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