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'Top Down' Approaches for the Study of Single-Cells: Micro-Engineering and Electrical Phenotype (2006)

Senez, Vincent ; Akalin, T. ; Arscott, S. ; [et al.]

s.l. ; Stafa-Zurich, Switzerland

Advances in science and technology Vol. 53 (Oct. 2006), p. 97-106

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ISSN: 1662-0356

Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008

Topics: Natural Sciences in General , Technology

Notes: Single-cell analysis is a very important field of research and is currently at the frontier ofphysical and biological sciences. Understanding how the phenotype of a single-cell arises from itsgenotype is a complex topic. Currently, the prevailing paradigm to analyze cellular functions is thestudy of biochemical interactions using fluorescence based imaging systems. However, theelimination of the labelling process is highly desirable to improve the accuracy of the analysis.Living cells are electromagnetic units; in as much they use electric mechanisms to control andregulate dynamic processes involved in inter alia signal transduction, metabolism, proliferation anddifferentiation. Recent developments in micro- and nanofabrication technologies are offering greatopportunities for the analysis of single cells; the combination of micro fluidic environments, nanoelectrodes/wires and ultra wide band electromagnetic engineering will soon make possible theinvestigation of local (submicrometer scale) dynamic processes integrating several events atdifferent time scales. In the paper, we present recent approaches which aim at investigating singlecellswith the help of MEMS and NEMS (Micro and Nano Electro Mechanical Systems) and ultrawide band (DC-THz) electromagnetic characterization techniques

Type of Medium: Electronic Resource

URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/42/transtech_doi~10.4028%252Fwww.scientific.net%252FAST.53.97.pdf

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Human mesenchymal stem cells successfully improve skin-substitute wound healing (2005)

Nakagawa, H. ; Akita, S. ; Fukui, M. ; [et al.]

Oxford, UK : Blackwell Science Ltd

British journal of dermatology 153 (2005), S. 0

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ISSN: 1365-2133

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Background Large or deteriorated skin defects are sometimes life threatening. There is increasing evidence that adult stem cells are useful for tissue regeneration. Human mesenchymal stem cells (hMSCs) are self-renewing and are potent in differentiating into multiple cells and tissues.Objectives To investigate the effects of hMSCs in cutaneous wound healing.Methods Wound healing was studied in an hMSC-populated porcine skin substitute, using a nude rat model to minimize immune reactions. Full-thickness skin and soft tissue defects of 1·5 × 1·5 cm in size, including the panniculus carnosus, were excised and covered with hMSCs and basic fibroblast growth factor (bFGF)-soaked skin substitutes and an evaluation was made of wound size, histology and protein expression at 3, 7 and 42 days after injury.Results The wound size was significantly smaller in the hMSC-treated groups (P 〈 0·01) and any dose of bFGF (1, 10, 100 μg) enhanced the healing (P 〈 0·01). The re-epithelialization markers integrin α3 and skin-derived antileucoproteinase were remarkably increased with the presence of bFGF in a dose-dependent manner, while the mesenchymal cell surface markers CD29 and CD44 were downregulated in a time-dependent manner. Human pancytokeratin, which does not cross-react with rat antigens, was observed by Western blotting at 38 kDa and 42 kDa from the hMSC-treated tissues on day 7. The expression levels were elevated by 10 μg bFGF (P 〈 0·01). The immunohistochemical expression of human pancytokeratin was only observed in the hMSC-treated groups.Conclusions These data suggest that hMSCs together with bFGF in a skin defect model accelerate cutaneous wound healing as the hMSCs transdifferentiate into the epithelium.