Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 2005-2009  (7)
Source
Material
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Application of periodontal ligament cell sheet for periodontal regeneration: a pilot study in beagle dogs (2005)
    Oxford, UK : Munksgaard International Publishers
    Journal of periodontal research 40 (2005), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Objective:  The ultimate goal of periodontal treatment is to regenerate the damaged periodontal support. Although periodontal ligament (PDL) cells are essential for periodontal regeneration, few studies have reported the transplantation of periodontal ligament cells to periodontal defects. We developed a new method to apply periodontal ligament cells as a sheet to the defect. The aim of this study was to investigate the periodontal healing after application of the periodontal ligament cell sheet in beagle dogs.Methods:  Autologous periodontal ligament cells were obtained from extracted premolars of each beagle dog. Periodontal ligament cell sheets were fabricated using a temperature-responsive cell culture dish. Dehiscence defects were surgically created on the buccal surface of the mesial roots of bilateral mandibular first molars of each dog. In the experimental group (five defects), periodontal ligament cell sheet with reinforced hyaluronic acid carrier was applied to the defect. Only the hyaluronic acid carrier was applied to the contralateral side as a control (five defects). Eight weeks after surgery, the animals were sacrificed and decalcified specimens were prepared. Healing of the periodontal defects was evaluated histologically and histometrically.Results:  No clinical signs of inflammation or recession of gingiva were observed in both experimental and control groups. In the experimental group, periodontal tissue healing with bone, periodontal ligament and cementum formation was observed in three out of five defects. In the control group, such periodontal tissue formation was not observed except in one defect. Histometric analysis revealed that the formation of new cementum in the experimental group was significantly higher than that in the control group.Conclusion:  The periodontal ligament cell sheet has a potential to regenerate periodontal tissue and may become a novel regenerative therapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.2005.00799.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction (2006)
    [s.l.] : Nature Publishing Group
    Nature medicine 12 (2006), S. 459-465 
    Details
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Mesenchymal stem cells are multipotent cells that can differentiate into cardiomyocytes and vascular endothelial cells. Here we show, using cell sheet technology, that monolayered mesenchymal stem cells have multipotent and self-propagating properties after transplantation into infarcted rat ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nm1391
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Engineering functional two- and three-dimensional liver systems in vivo using hepatic tissue sheets (2007)
    [s.l.] : Nature Publishing Group
    Nature medicine 13 (2007), S. 880-885 
    Details
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Hepatic tissue engineering using primary hepatocytes has been considered a valuable new therapeutic modality for several classes of liver diseases. Recent progress in the development of clinically feasible liver tissue engineering approaches, however, has been hampered mainly by insufficient ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nm1576
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    A Novel Cell Co-Culture Method Utilizing Thermo-Responsive Surface (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 342-343 (July 2007), p. 221-224 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In this work, we developed a novel patterned co-culture method with thermo-responsivepoly(N-isopropylacrylamide) (PIPAAm) and poly(N-ρ-vinylbenzyl-Ο-β-D-galactopyranosyl-(1→4)-D-gluconamide) (PVLA) inducing active hepatocyte attachment. Patterned graft of PIPAAmonto PS dishes was carried out by electron beam irradiation using cover-glass as a photomask.PVLA was only coated onto PIPAAm-ungrafted domain because of hydrated hydrophilic propertyof PIPAAm at below the LCST. Analysis by attenuated total reflection-Fourier transform infraredand electron spectroscopy for chemical analysis revealed that PIPAAm and PVLA weresuccessfully grafted and coated on surfaces of PS dishes. PIPAAm-grafted surface exhibiteddecreasing contact angle by changing temperature from 37 to 20°C, while PVLA-coated PS andnon-treated PS had negligible contact angle changes with temperature alternation. Atomic forcemicroscopy (AFM) results showed that PIPAAm-grafted and PVLA-coated PS had smoothersurfaces than that of ungrafted PS dishes. After culture for 12 hours, hepatocytes were well attachedon PVLA-coated domain. Hepatocytes adherent on PIPAAm-grafted domain were detached bydecreasing temperature. And then, fibroblasts were seeded onto PIPAAm pattern-grafted domain.Fibroblasts were only attached and spread onto PIPAAm-grafted domain. Co-cultured hepatocytesshowed better differentiated function of albumin expression compared to homotypic hepatocyteculture
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.221.pdf
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Rapid Cell Sheet Recovery by Successive Grafting of PHEMA and PIPAAm onto TCPS Dish Surfaces (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 342-343 (July 2007), p. 245-248 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Rapid recovery of cell sheets is considered important to maintain the biological functionand viability of recovered cell sheets. To accelerate required culture substrate hydrophilic/hydrophobic structural changes in response to culture temperature alteration, poly(2-hydroxyethylmethacrylate) (PHEMA) and poly(N-isopropylacrylamide) (PIPAAm) were grafted successivelyonto tissue culture polystyrene (TCPS) dishes by electron beam irradiation. Analysis by attenuatedtotal reflection-Fourier transform infrared revealed that PHEMA and PIPAAm were successfullygrafted to surfaces of TCPS dish. PIPAAm-PHEMA-grafted TCPS (PIPAAm-PHEMA-TCPS)dishes were compared with PIPAAm-grafted TCPS dishes for cell sheet detachment experiments.Approximately 75 min was required to completely detach cell sheets from PIPAAm-TCPS dish,compared to only 13 min to detach cell sheets from PIPAAm-PHEMA-TCPS dish, which issuccessively grafted with PHEMA and PIPAAm. PHEMA is a well-known as a high hygroscopicpolymer. In the case of PIPAAm-PHEMA-TCPS dish, PHEMA layer acted as a water pool toaccelerate the hydration of PIPAAm layer due to the effective and simultaneous water supply toPIPAAm layer, resulting in rapid hydration of grafted PIPAAm molecules and detachment of cellsheet compare to PIPAAm-TCPS dish without PHEMA layer
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.245.pdf
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Thermo-Intelligent Surfaces for Cell Culture (2006)
    s.l. ; Stafa-Zurich, Switzerland
    Advances in science and technology Vol. 53 (Oct. 2006), p. 70-73 
    Details
    ISSN: 1662-0356
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Natural Sciences in General , Technology
    Notes: In order to avoid several complications resulted from biodegradable scaffolds or single cellinjection, we have developed “cell sheet engineering”. Our concept is novel tissue reconstructionnot from single cells but from cell sheets. In order to prepare cell sheets, temperature-responsiveculture dishes are utilized. Since temperature-responsive polymers are covalently grafted on thedishes, various types of cells adhere and proliferate on them at 37°C, but are spontaneouslydetached only by reducing temperature below 32°C without any need for proteolytic enzyme. Allthe confluent cells are noninvasively harvested as a single contiguous cell sheets with intact cell-celljunctions and deposited extracellular matrix. We have utilized these harvested cell sheets for varioustissue reconstructions including ocular surfaces, periodontal ligament, cardiac patches as well asbladder
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/42/transtech_doi~10.4028%252Fwww.scientific.net%252FAST.53.70.pdf
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Nanotechnology-Based Cell Sheet Engineering for Regenerative Medicine (2006)
    s.l. ; Stafa-Zurich, Switzerland
    Advances in science and technology Vol. 53 (Oct. 2006), p. 74-78 
    Details
    ISSN: 1662-0356
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Natural Sciences in General , Technology
    Notes: In order to realize true regenerative medicine, we have developed a novel technology forthe reconstruction of tissues and organs by utilizing intelligent materials includingtemperature-responsive polymers. We developed temperature-responsive culture surfaces, onwhich temperature-responsive polymers are covalently immobilized. Cells are cultured on thesurfaces at 37°C, and harvested as transplantable cell sheets by reducing temperature to 20°C. Withthese cell sheets we regenerate various kinds of tissues such as cornea and heart
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/42/transtech_doi~10.4028%252Fwww.scientific.net%252FAST.53.74.pdf
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...