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  • 2000-2004  (4)
  • 1995-1999  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Comparisons of IgE, IgG, and IgG4 responsiveness to Dermatophagoides farinae in children by immunobulotting (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 51 (1996), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Although asthmatic children are often sensitized to the house-dust mite (HDM) during early childhood, it is not clear what antigenic components are associated with the early immune response of these children. To investigate this problem, we evaluated IgE, IgG, and IgG4 antibody responses to Dermatophagoides farinae (Df) by immunoblotting among three groups of children: group I aged 0–4 years, group II aged 5–9 years, and group III aged 10–14 years. In the group I subjects, positive IgE-binding reactions to 15– and 25-kDa components were found in 76% and 44% of sera, respectively. Those to other components were generally low in frequency. In contrast, positive IgG-binding reactions to 15– and 25-kDa components were found in 44% and 24% of sera, and those to 30– and 110-kDa components in 48% and 88% of sera, respectively. Positive IgG4 reactions to 15– and 25-kDa components were found in 48% and 24%, respectively, and those to other components were very low. Positive IgE-binding reactions to these components gradually became more frequent with increasing age in groups II and III, while IgG and IgG4 reactivities were not markedly different in these age groups. These results suggest that the 15-and 25-kDa proteins of Df are important antigens associated with the initial IgE response to HDM in early childhood, while the 30– and 110-kDa proteins are associated with IgG and 15-kDa components with IgG4.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1996.tb04491.x
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  • 2
    Electronic Resource
    Electronic Resource
    Mutation screening of interferon regulatory factor 1 gene (IRF-1) as a candidate gene for atopy/asthma (2000)
    Oxford BSL : Blackwell Science Ltd
    Clinical & experimental allergy 30 (2000), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: IL-4 gene cluster on chromosome 5 contains several candidate genes for atopy and asthma. Several independent studies have shown evidence for linkage between the markers flanking IL-4 gene cluster and asthma and/or asthma-related traits. Interferon regulatory factor 1 (IRF-1) is located approximately 300 kb telomeric to IL-4 and recent study reveals that IRF-1 deficiency results in an elevated production of Th2-related cytokines and a compensatory decrease in the expression of native cell- and Th1-related cytokines.〈section xml:id="abs1-2"〉〈title type="main"〉ObjectiveTo determine if there are any mutations associated with the development of atopy and asthma present in the coding exons and 5′ flanking region of the IRF-1 gene.〈section xml:id="abs1-3"〉〈title type="main"〉Methods and resultsWe have screened the promoter and coding regions of the IRF-1 gene in atopic asthmatics and controls by SSCP method. We found three novel nuclear variants (the −300G/T and 4396 A/G polymorphisms and the 6355G 〉 A rare variant) in the IRF-1 gene. No variants causing amino acid alterations of IRF-1 were detected. The −300G/T polymorphism was in nearly complete linkage disequilibrium with the 4396 A/G polymorphism. An association between the 4396 A 〉 G polymorphism and atopy/asthma was examined by transmission disequilibrium test in 81 asthmatic families. Either of 4396 A or 4396G alleles was not significantly preferentially transmitted to atopy- or asthma-affected children.〈section xml:id="abs1-4"〉〈title type="main"〉ConclusionThe IRF-1 gene is less likely to play a substantial role in the development of atopy and asthma in the Japanese population.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2000.00916.x
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  • 3
    Electronic Resource
    Electronic Resource
    Comparison of basophil histamine release, eosinophil cationic protein and non-specific airway responsiveness between mite-sensitive asthmatic and non-asthmatic children and non-allergic controls (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 26 (1996), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: To understand the relevance of allergy to the development of asthma in children, we examined basophil hislamine release (HR) with Df antigen, blood cosinophii counts, serum eosinophil cationic protein (ECP) levels, and bronchial responsiveness to methacholine (PC20) in three groups of children, including 36 asthmatics with high RAST titre for Df (group 1), 36 non-asthmatics with similarly high RAST titre for Df (group 2) and 21 non-asthmatics with negative RAST titre for Df (group 3). The amount of Df antigen inducing 50% HR from basophils did not vary significantly between group 1 and 2 (P〉0.05), while none of the cells responded to higher concentrations of Df in group 3. The mean number of blood eosinophils and level of serum ECP were highest in group 1, and lowest in group 3, with group 2 being intermediate, and the differences were significant between all three groups (P〈0.01). The mean PC20 value was the lowest in group 1, intermediate in group 2, and the highest in group 3, and the differences were significant between all three groups (P〈0.01). While correlation studies showed that PC20 values of group 2 subjects signficantly correlated with their eosinophil numbers (r=–0.48, P〈0.01) and ECP levels (r =–0.49, P 〈 0.01), such correlations were not found in group 1 subjects. These results suggest that the degree of the eosinophilic inflammation caused by the allergic reaction to mites is an important factor in determining the clinical expression of asthma in atopic subjects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1996.tb00627.x
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  • 4
    Electronic Resource
    Electronic Resource
    New polymorphisms of haematopoietic prostaglandin D synthase and human prostanoid DP receptor genes (2002)
    Oxford, UK : Blackwell Science, Ltd
    Clinical & experimental allergy 32 (2002), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Prostaglandin D2 (PGD2), a major cyclo-oxygenase metabolite of arachidonic acid in mast cells, induces bronchoconstriction in the human lung. It has been reported that mice lacking PGD receptor fail to develop the bronchial hyper-responsiveness upon ovalbumin challenge, suggesting that PGD2 functions as a mediator of allergic asthma.Objective To determine if there are any mutations associated with the development of asthma in the haematopoietic prostaglandin D synthase (H-PGDS) gene and the human prostanoid DP receptor (PTGDR) gene.Methods and results We screened the 5′flanking and coding regions of the H-PGDS gene and the PTGDR gene by direct sequence. We identified one variant in intron 2 (IVS2 + 11 A 〉 C) and one variant in intron 3 (IVS3 + 13T 〉 C) of the H-PGDS gene, and two variants in the 5′flanking region of the PTGDR gene (−197T 〉 C and −2C 〉 T). The IVS3 + 13T 〉 C and −197T 〉 C variants were rare, appearing only once in 48 subjects. transmission disequilibrium test (TDT) analysis of 144 asthmatic families revealed that the IVS2 + 11 A allele of the H-PGDS gene was significantly transmitted preferentially to asthma-affected children (P = 0.0056), but no association was observed between −2C/T polymorphism of the PTGDR gene and asthma (P 〉 0.05).Conclusion Our results suggest that the IVS2 + 11 A/C allele may be involved in the development of asthma in the Japanese population.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.0022-0477.2001.01261.x
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  • 5
    Electronic Resource
    Electronic Resource
    No evidence for association between the −112G/A polymorphism of UGRP1 and childhood atopic asthma (2003)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 33 (2003), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Susceptibility to asthma is known to involve genetic factors. Genome-wide screens have indicated that the chromosome 5q31–q34 region is linked to and/or associated with asthma. A new gene, named UGRP1 and reported by Niimi et al., encodes uteroglobin-related protein and is expressed in the lung and trachea. Niimi et al. showed the −112G/A polymorphism of the UGRP1 gene to be associated with asthma in a case–control study.Objective The objective of the present study was to replicate this association and confirm the possible role of the UGRP1−112G/A polymorphism in the aetiology of childhood asthma in a Japanese population.Methods and results We conducted a transmission disequilibrium test (TDT) in 131 families identified through paediatric patients being treated for asthma. A case–control study was also carried out by comparing the probands and 137 unrelated non-atopic non-asthmatic Japanese children and 211 unrelated healthy Japanese adults. The −112G/A polymorphism was genotyped by the PCR-RFLP method. The TDT revealed that the −112A allele was not preferentially transmitted to asthma-affected children (P=0.85). Neither the presence of at least one A allele in an individual's genotype (sum of the G/A and A/A genotypes) nor the −112A allele was more prevalent among the asthma subjects than among the control subjects.Conclusion Our findings indicate that the UGRP1−112G/A polymorphism does not play a substantial role in genetic predisposition to childhood asthma in this Japanese population.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2003.01703.x
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  • 6
    Electronic Resource
    Electronic Resource
    An association study of asthma and total serum immunoglobin E levels for Toll-like receptor polymorphisms in a Japanese population (2004)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 34 (2004), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background The prevalence of atopic diseases has been increasing in developed countries. This could be explained by the hygiene hypothesis, which states that exposure to specific infections or endotoxins during infancy drives the maturing immune system towards a Th1 phenotype and away from the Th2 phenotype, which is associated with allergic diseases. Toll-like receptors (TLRs) play important roles in the signalling of many pathogen-related molecules and endogenous proteins associated with immune activation.Objective The aim of the present study was to investigate whether polymorphisms in genes encoding TLRs are associated with asthma or total serum IgE levels.Methods We screened the 5′ flanking and coding regions of the TLR2,TLR3, TLR4, and TLR9 genes for polymorphisms by direct sequencing of DNA from 32 asthmatics, and analysed the effect of the polymorphisms on the development of atopic asthma and on total serum IgE levels.Results We identified 16 variants in TLRs. The transmission disequilibrium test of the families revealed that none of the alleles or haplotypes were associated with asthma or total IgE levels (P〉0.05). However, we found an insertion/deletion polymorphism in the 5′ untranslated region of TLR2, and an expression construct containing the deletion allele showed lower luciferase activity than the wild-type alleles, suggesting that the deletion allele has reduced transcriptional activity.Conclusion Our results indicate that polymorphisms in TLRs are not likely to be associated with the development of atopy-related phenotypes in a Japanese population.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.2004.01839.x
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  • 7
    Electronic Resource
    Electronic Resource
    Mixing enhancement in axisymmetric turbulent isothermal and buoyant jets (1996)
    Springer
    Experiments in fluids 20 (1996), S. 279-290 
    Details
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Measurements of the velocity and concentration in axisymmetric, turbulent, isothermal and buoyant jets have been performed with laser-Doppler velocimetry and planar and point laser-induced fluorescence to quantify the mixing enhancement achieved by periodic forcing when the jet exit has a fully-developed turbulent pipe flow, a situation less well-studied than the case of laminar initial conditions. It was found that forcing at Strouhal numbers around 0.6 enhances mixing in the developing region of the jet and this enhancement increased with increasing amplitude of excitation, consistent with results of initially-laminar jets. The initial turbulence intensity did not have any effect, but an increase in the initial lengthscale of the turbulence, controlled by a perforated plate inside the nozzle, caused faster mixing. In agreement with previous experiments, the initial conditions of the jet did not affect the far-field rate of decay, but the jet-fluid concentration there was significantly reduced by forcing due to the increased mixing during the early stages of development, an effect that can be described by a smaller virtual origin in decay laws of jet decay. These results are independent of the Froude number because the initial conditions have an influence only in the early stages where the flow is still momentum dominated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00192673
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  • 8
    Electronic Resource
    Electronic Resource
    Lysinuric protein intolerance and systemic lupus erythematosus (1998)
    Springer
    European journal of pediatrics 157 (1998), S. 130-131 
    Details
    ISSN: 1432-1076
    Keywords: Key words Lysinuric protein intolerance ; Systemic lupus erythematosus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report lysinuric protein intolerance (LPI) and systemic lupus erythematosus (SLE) in a 14-year-old girl. The co-existence of LPI and SLE in the same patient has been reported before and suggests that SLE is directly connected with LPI. Conclusion Possibly in LPI the metabolic derangement can cause immunological abnormalities. Therefore, when multisystem involvement is observed in LPI patients, SLE should be considered as a rare complication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004310050784
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  • 9
    Electronic Resource
    Electronic Resource
    Time Dependence of Degradation Phenomena of Plain Woven AFRP in Hot, Wet Environmental Exposure (1998)
    Springer
    Mechanics of time-dependent materials 2 (1998), S. 351-369 
    Details
    ISSN: 1573-2738
    Keywords: acoustic method ; hot/wet environment ; plain woven materials
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Plain woven AFRP samples were immsersed in hot water at 80°C and exposed to humid air at 80°C, 80% RH for 300~days. Furthermore, they were tested by a tensile method to investigate time dependence of their degradation. The longer the immersion time, the greater the degradation of tensile strength. But on comparison with samples exposed to hot humid air, those immersed in hot water showed more degradation, although both conditions had the same temperature. Hot water caused the yarn/resin interface to degrade and led to delamination. Warps, which are weakened by absorption, were further damaged by delamination. Damaged warps broke and final fracture occurred at a low level of stress. At an average stress level, AE event rate of samples in hot, wet conditions was lower than that of virgin samples. This was because cracks occurred in the resin-rich area during water/moisture absorption, i.e., fracture occurred before tensile test. The change of fracture by water/moisture absorption in a hot, wet environment could also be revealed by the AE method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009807309064
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