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  • 1
    Digitale Medien
    Digitale Medien
    Optical measurement of crack propagation with high resolution (1993)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 64 (1993), S. 1822-1824 
    Details
    ISSN: 1089-7623
    Quelle: AIP Digital Archive
    Thema: Physik , Elektrotechnik, Elektronik, Nachrichtentechnik
    Notizen: A new optical method is established to measure the crack length and its velocity in transparent crystals by utilizing the total reflection of light beam at the cleavage crack plane. The optical system is constructed by using a laser, a PIN photodiode sensor, and a wave memory. With the apparatus the crack propagation velocity can be measured at any desired position inside the specimen with a time resolution of less than 1 μs. The measurement on a cleavage crack in NaCl reveals that the maximum speed of the crack is about 800 m/s and the fluctuation of the velocity occurs prior to the arrest.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.1144017
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  • 2
    Digitale Medien
    Digitale Medien
    DX centers in Si-doped AlxGa1−xAs grown by metalorganic chemical vapor deposition (1990)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 68 (1990), S. 1669-1673 
    Details
    ISSN: 1089-7550
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: Three levels corresponding to photo energies E0(=1.7 eV), E1(=1.43 eV), and E2 (=energy gap) were observed in differential photocapacitance measurements of Si-doped AlxGa1−xAs grown by metalorganic chemical vapor deposition (0.23〈x〈0.4). On the other hand, E1 and E2 were observed but E0 was not observed in the differential photoconductance measurements. Both photon energies E1 and E2 cause persistent photoconductance (PPC) and the electron concentration coincides with the concentration of Si atoms. Our results support the broken-bond model proposed by D. J. Chadi and K. J. Chang [Phys. Rev. B 39, 10063 (1989)].
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.346650
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  • 3
    Digitale Medien
    Digitale Medien
    An analytical representation of the lowest potential energy surface for the reaction O(3P)+HCl (X 1Σ+)→OH(X 2Π)+Cl(2P) (1991)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 95 (1991), S. 6421-6428 
    Details
    ISSN: 1089-7690
    Quelle: AIP Digital Archive
    Thema: Physik , Chemie und Pharmazie
    Notizen: We present a new analytical representation of the lowest 3A‘ energy surface for the reaction O(3P)+HCl(X 1Σ+)→OH(X 2Π)+Cl(2P). This surface is derived by fitting ab initio calculations, and adjustments are made to the barrier height so that rate constants derived from accurate quantum scattering calculations match the room temperature thermal rate constants. Contributions to thermal and state resolved rate constants arising from reaction on the lowest 3A' surface are also considered. Comparisons of thermal and state resolved rate constants with experiment are good except at high temperature where the 3A' contributions are significant.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.461562
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  • 4
    Digitale Medien
    Digitale Medien
    Serological and immunohistochemical detection of human herpesvirus 8 in Kaposi's sarcoma after immunosuppressive therapy for bullous pemphigoid (2001)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 145 (2001), S. 0 
    Details
    ISSN: 1365-2133
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Kaposi's sarcoma (KS) developed in an 87-year-old human immunodeficiency virus-negative woman from Hokkaido island 4 months after oral administration of prednisolone for the treatment of bullous pemphigoid (BP), and rapidly disseminated to almost the entire body within 2 months. The open reading frame (ORF) 59 and ORF73 proteins encoded by human herpesvirus 8 (HHV-8) were detected immunohistochemically in the nuclei of the tumour cells of KS. The protein coded by ORF73, latent protein, was detected in most of the nuclei of the tumour cells, but only a few tumour nuclei were positive for the ORF59 protein, a lytic protein expressed during active infection. The antibodies against both lytic and latent proteins of HHV-8 were detected retrospectively in the serum 4 months before the appearance of KS and before prednisolone therapy had been started. Immunosuppression associated with the treatment for BP possibly activated latent HHV-8 infection and induced the development of KS.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1365-2133.2001.04413.x
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  • 5
    Digitale Medien
    Digitale Medien
    Comparative strength of metal–ceramic and metal–composite bonds after extended thermocycling (2004)
    Oxford, UK : Blackwell Science Ltd
    Journal of oral rehabilitation 31 (2004), S. 0 
    Details
    ISSN: 1365-2842
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: summary  The relative strengths of ceramic-to-metal and composite-to-metal bonds were compared after prolonged thermocycling. A total of 104 cast discs were produced from a gold alloy (Pontor LFC). A ceramic material (Duceragold) was fused to 24 discs to assess the strength of the metal–ceramic bond. An indirect composite material (New Metacolor Infis) was bonded to the remaining discs after surface preparation by Rocatec tribochemical coating, tin plating and priming with a phosphate conditioner [10-methacryloyloxydecyl dihydrogen phosphate (MDP), Cesead II], priming with a thione conditioner (V-Primer) or no treatment (unprimed control). Shear bond strengths were determined before and after thermocycling at 20 000 and 100 000 cycles. Pre-thermocycling bond strengths were ranked in the order: metal–ceramic (40·5 MPa); Rocatec treatment (33·1 MPa) and tin plating–MDP (31·0 MPa); V-Primer (20·9 MPa); and control (11·9 MPa). The bond strengths of the first three groups were not significantly different after 20 000 thermocycles, whereas those of the V-Primer and control groups were significantly reduced. After extended thermocycling (100 000 cycles) the metal–ceramic group had the highest mean shear bond strength (28·5 MPa; P 〈 0·05), followed by the Rocatec (23·9 MPa) and tin plating–MDP (22·1 MPa) groups. The metal–ceramic bond was the most durable, although its strength was reduced by 29·6% after extended thermocycling. On the basis of these results, we recommend the Rocatec and tin plating–MDP systems for composite-to-metal bonding. Metal–ceramic bonding, however, is superior to metal–composite bonding within the limitation of the current experiment.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2842.2004.01391.x
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  • 6
    Digitale Medien
    Digitale Medien
    Lichenoid skin lesions as a sign of β2-microglobulin-induced amyloidosis in a long-term haemodialysis patient (1993)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 128 (1993), S. 0 
    Details
    ISSN: 1365-2133
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: We report a case of β2-microglobulin-induced amyloidosis. The patient was a 40-year-old man suffering from non-amyloid nephropathy, who had been treated by haemodialysis for 20 years. Lichenoid skin lesions, consisting of groups of pin-head-sized shiny papules, were present on the arms and trunk. On histological examination, amyloid deposits were present, principally in the dermal papillae, hut also around the sweat ducts and hair follicles. The amyloid displayed potassium-permanganate-resistant Congo red affinity, and green birefringence under polarized light, Immuno-histochemically, β2-microglobulin was demonstrated in the lesions, confirming that they were a manifestation of β2-microglobulin-associated amyloidosis. Skin lesions of this type have not been reported previously in β2-microglobulin-associated amyloidosis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2133.1993.tb00266.x
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  • 7
    Digitale Medien
    Digitale Medien
    Substance P induces intracellular calcium increase and translocation of protein kinase C in epidermis (1992)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 127 (1992), S. 0 
    Details
    ISSN: 1365-2133
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Substance P is a neuropeptide present in, and released from, peripheral C nerve endings. The presence of substance P-positive nerve fibres in the epidermis has been reported. We investigated the effect of substance P on the transmembrane signalling system of pig epidermal keratinocytes. Treatment of pig epidermis with substance P resulted in an increase in inositol 1,4,5-trisphosphate (IP3), and in intracellular free calcium. The treatment also resulted in translocation of protein kinase C from a cytosol to a membrane fraction. Substance P, however, did not affect the β-adrenergic- or histamine (H2)- adenylate cyclase responses of the epidermis. Neither forskolin-induced, nor cholera toxin-induced cyclic AMP accumulation were affected by substance P treatment. These results are consistent with the view that substance P stimulates phosphatidylinositol-4,5-bisphosphate (PIP2) hydrolysis of keratinocytes, resulting in IP3-Ca2+ and diacylglycerol-protein kinase C signal activation. Although protein kinase C is known to affect the epidermal adenylate cyclase system, no evidence for such ‘cross-talk regulation’ was detected in keratinocytes by substance P treatment.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2133.1992.tb14872.x
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  • 8
    Digitale Medien
    Digitale Medien
    Adenylate cyclase induces intracellular calcium increase in single human epidermal keratinocytes measured by fluorescence microscopy using Fura 2-AM (1992)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 127 (1992), S. 0 
    Details
    ISSN: 1365-2133
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Intracellular calcium ([Ca2+]i) is an important second messenger of extracellular signals to induce various cellular responses. Extracellular and intracellular Ca2+ are considered to be important for cellular differentiation and proliferation of epidermal keratinocytes. Several mechanisms which increase [Ca2+]i have been demonstrated in various tissues, but in epidermal keratinocytes these mechanisms are poorly understood. In epidermal keratinocytes the adenylate cyclase-cyclic AMP response is thought to regulate cell proliferation and differentiation. However, the series of reactions which follow the cyclic AMP response remain unknown. Beta-adrenergic agonists increase [Ca2+]i in cultured epidermal keratinocytes, and we have therefore studied whether stimulation of keratinocyte adenylate cyclase could induce [Ca2+]i increase, by using fluorescence microscopy with Fura 2-AM.Adenosine and histamine, which are known to be keratinocyte adenylate cyclase receptor agonists, induced transient [Ca2+]i increase, as did epinephrine. In addition, forskolin, a direct adenylate cyclase activator, and dibutylyl-cyclic AMP also induced an increase in [Ca2+]i. In a calcium-free medium epinephrine, adenosine, histamine and dibutylyl-cyclic AMP induced an increase in [Ca2+]i. These results suggest that cyclic AMP in human epidermal keratinocytes regulates [Ca2+]i, which is released from intracellular stores.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2133.1992.tb14871.x
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  • 9
    Digitale Medien
    Digitale Medien
    Immunohistochemical analysis of human skin using antispectrin and anti-β-fodrin antibodies (1990)
    Springer
    Archives of dermatological research 282 (1990), S. 274-277 
    Details
    ISSN: 1432-069X
    Schlagwort(e): Spectrin ; Fodrin ; Membrane skeleton ; Keratinocytes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00371651
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  • 10
    Digitale Medien
    Digitale Medien
    Membrane expression of annexin I is enhanced by calcium and TPA in cultured human keratinocytes (2000)
    Springer
    Archives of dermatological research 292 (2000), S. 496-499 
    Details
    ISSN: 1432-069X
    Schlagwort(e): Key words Keratinization ; Cornified envelope ; 12-O-Tetradecanoyl phorbol 13-acetate (TPA) ; Epinephrine
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract  Annexin I plays an important role in the process of keratinization as a component of the cornified envelope. To elucidate the function of annexin I in keratinization, we investigated the effects of calcium, epinephrine, hydrocortisone, and 12-O-tetradecanoyl phorbol 13-acetate (TPA) on the expression and localization of annexin I in cultured human keratinocytes. Normal human keratinocytes were cultured in serum-free culture medium (0.15 mM calcium) until 70% confluence. After incubation with a higher concentration of calcium (1.8 mM), TPA (100 nM), epinephrine (50 ÌM), or hydrocortisone (10 ÌM) for 24 h, the expression of annexin I protein and mRNA was examined using immunofluorescence, Western blot, and Northern blot techniques. Immunofluorescence microscopy showed increased membrane staining of annexin I by calcium, which was inhibited by the addition of epinephrine or hydrocortisone. Western blotting confirmed elevated annexin I on the cell membrane. It was increased in the cell membrane fraction, but not in the cytosol fraction. Interestingly, the mRNA level of annexin I was slightly reduced after incubation with calcium, whereas TPA upregulated both membrane expression and the mRNA level. Secretion of annexin I was increased by TPA but inhibited by calcium. Because calcium and TPA are known to promote keratinization, our data suggest that annexin I expression on the cell membrane is involved in the process of keratinization.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004030000172
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