ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

In vivo study of phagocytosis, intracellular survival and multiplication of Flavobacterium psychrophilum in rainbow trout, Oncorhynchus mykiss (Walbaum), spleen phagocytes (2001)

Decostere, A ; D'Haese, E ; Lammens, M ; [et al.]

Oxford UK : Blackwell Science Ltd

Journal of fish diseases 24 (2001), S. 0

add to mindlist on the mindlist

Details

ISSN: 1365-2761

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology , Medicine

Notes: The intracellular behaviour of a Flavobacterium psychrophilum strain, ingested by spleen phagocytes of rainbow trout, Oncorhynchus mykiss, of different ages, was assessed in vivo. Three groups of rainbow trout weighing 1 g (aged 10 weeks), 25 g (aged 20 weeks) and 300 g (aged 15 months), respectively, were injected intraperitoneally with 1 × 106 cfu of a F. psychrophilum strain. It was found that only fry, aged 10 weeks, displayed clinical signs and suffered mortality. Bacteriological colony plating of different organs demonstrated that the spleen and to a lesser extent the kidney of only the fry were affected. The number of colony forming units per gram of spleen tissue increased with time. No bacteria were found in the trout aged 5 months and older. Light microscopical examination and epifluorescence microscopy revealed that the fry spleen phagocytes contained an increasing number of viable intracellular F. psychrophilum bacteria over time. Again, no bacteria were encountered in the phagocytes collected from older fish.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1365-2761.2001.00322.x

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Peripheral primitive neuroectodermal tumour of the cervix (2000)

Pauwels, P. ; Ambros, P. ; Hattinger, C. ; [et al.]

Springer

Virchows Archiv 436 (2000), S. 68-73

add to mindlist on the mindlist

Details

ISSN: 1432-2307

Keywords: Key words Cervix ; Peripheral primitive neuroectodermal tumour ; Ewing’s tumour pathology ; Immunohistochemistry ; Cytogenetics

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Peripheral primitive neuro-ectodermal tumours (PNET) of the cervix are very rare. Here, we report the clinical, pathological, immunohistochemical and genetic features of a case of a PNET located in the cervix. Hysterectomy revealed a cervical tumour. On microscopic examination, a vaguely lobular arrangement of uniformly appearing neoplastic cells, with round to oval nuclei, distinct nuclear membranes and a clear, moderately glycogen-rich cytoplasm was seen. Cells stained positive for LEU 7, S 100, monoclonal NSE and particularly for MIC2. Neurogenic differentiation was also seen by electron microscopic examination. The genetic hallmark of PNET, a 22q12 rearrangement was demonstrated by fluorescence in situ hybridisation experiments, supporting the diagnosis. Awareness of the existence of primary PNET of the cervix is important to avoid confusion with other tumours of the cervix.