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  • 1
    Electronic Resource
    Electronic Resource
    Glucose-induced loss of glycosyl-phosphatidylinositol-anchored membrane regulators of complement activation (CD59, CD55) by in vitro cultured human umbilical vein endothelial cells (2000)
    Springer
    Diabetologia 43 (2000), S. 1039-1047 
    Details
    ISSN: 1432-0428
    Keywords: Keywords CD59 ; CD55 ; CD46 ; endothelial cells ; glucose ; diabetes mellitus ; vascular complications ; MAC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Aims/hypothesis. This study examines whether increased glucose concentrations are responsible for a decreased expression of membrane regulators of complement activation molecules. The effect of high glucose in determining an increase in membrane attack complex deposition on endothelial cells was also investigated. Methods. Endothelial cells were isolated from umbilical cord tissue, cultured in the presence of increased concentrations of glucose, and the expression of CD46, CD55, and CD59 was detected by ELISA (enzyme-linked immunosorbent assay) and by flow cytometry. Glucose-treated endothelial cells were also incubated with antiendothelial cell antibodies and fresh complement to assess the amount of membrane attack complex formation. Results. High concentrations of glucose decreased the expression of CD59 and CD55 by endothelial cells in a time-dependent and glucose concentration-dependent manner without affecting CD46 expression. High concentrations of soluble CD59 were found in the supernatants of cells treated with high glucose. The decrease in CD59 expression induced by high glucose concentrations was reversed by coincubation of cells with a calcium channel blocking agent (Verapamil). All of these effects were not reproduced by osmotic control media. Cells treated with concentrations of high glucose were more susceptible to complement activation and membrane attack complex formation after exposure to antiendothelial cell antibodies. Conclusion/Interpretation. We speculate that hyperglycaemia could directly contribute to a loss of CD59 and CD55 molecules through a calcium-dependent phosphoinositol-specific phospholipase C activation and subsequent regulation of cell wall expression of GPI-anchored proteins. This phenomenon could facilitate the activation of a complement pathway and could play a part in the aetiology of endothelial dysfunction in diabetes. [Diabetologia (2000) 43: 1039–1047]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250051487
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Calibration of optical tweezers with differential interference contrast signals (2002)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 73 (2002), S. 1687-1696 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A comparison of different calibration methods for optical tweezers with the differential interference contrast (DIC) technique was performed to establish the uses and the advantages of each method. A detailed experimental and theoretical analysis of each method was performed with emphasis on the anisotropy involved in the DIC technique and the noise components in the detection. Finally, a time of flight method that permits the reconstruction of the optical potential well was demonstrated. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1460929
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Investigation of the near wake of a propeller using particle image velocimetry (2000)
    Springer
    Experiments in fluids 29 (2000), S. S227 
    Details
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The investigation of a propeller wake is carried out in a cavitation tunnel using particle image velocimetry. To achieve high spatial resolution, a multigrid adaptive cross-correlation algorithm is used for image analysis. The contributions of the viscous wake, originated by the boundary layer on the blades of the trailing vortex sheets, as well as of the vorticity distribution are resolved and discussed for different angular positions of the blade. The near wake flow is characterised by strong deformations, due to the bending of the blade wake sheets, to the contraction of the slipstream and to the trajectory of the tip vortex. The turbulent diffusion and the viscous dissipation rapidly lead to broadening and decay of the trailing edge wake. Furthermore, secondary tip vortex structures are also pointed out.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003480070025
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    Paper (German National Licenses)
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