Library

Notes: Background Prominent infiltration of eosinophils in airway mucosa is the pathognomonic sign of asthma. The role of airway epithelial cells in eosinophil infiltration, however, has not been fully elucidated.Objective The aim of this study is to develop a new in vitro transmigration system composed of airway epithelial cells and extracellular matrix, and to investigate the role of airway epithelial cells in eosinophil infiltration.Methods A layer of type I collagen gel was formed in Netwell™, and BEAS-2B bronchial epithelial cells were cultured on the gel. Then the wells covered with epithelial monolayer were filled with medium, inverted, and new upper chambers were constructed on the gel side by applying a ring cap. After further incubation with or without exogenous cytokines for 48 h, eosinophils or neutrophils were loaded in upper chambers (the gel side) and cells transmigrated to lower chambers (the epithelial cell side) were counted. Immunohistochemical analyses were also performed.Results While a simple collagen gel hardly promoted eosinophil migration even in the presence of eotaxin or RANTES, significant numbers of eosinophils migrated to lower chambers in the presence of the epithelial cells. Replacement of medium in the lower chamber (the epithelial cell side) with fresh medium, addition of exogenous eotaxin or RANTES in the upper chamber (the gel side), or pre-treatment of eosinophils with anti-CCR3 all inhibited transmigration. We found that the epithelial cells produced and deposited extracellular matrix proteins such as type IV collagen onto the type I collagen gel. Separately, we found that type IV collagen itself was capable of enhancing eotaxin-induced eosinophil migration in a standard chemotaxis assay. Neutrophils also efficiently migrated in the present transmigration system. Pre-treatment of epithelial cells with TNF-α and IL-4 enhanced eosinophil transmigration, while that of neutrophils was enhanced by TNF-α but suppressed by IL-4.Conclusion By utilizing a new in vitro transmigration system mimicking the airway mucosa, we have demonstrated that airway epithelial cells play an essential role in transmigration of eosinophils and that multiple factors such as chemokines, extracellular matrix proteins and exogenous inflammatory cytokines are involved in efficient transmigration.

Notes: We report a 52-year-old patient with cutaneous angiomas on the trunk in association with angiotropic lymphoma involving the nervous system. The lesional skin showed proliferation of atypical B cells in the capillaries. The diagnosis of angiotropic lymphoma with neurological deficits is often difficult before death. Thus, skin biopsy in this case proved to be diagnostic of the central nervous system disease without the risk of brain biopsy. The diagnosis of angiotropic lymphoma in the future may be achievable by biopsy of coincidental angiomas.

Notes: Abstract The steady, developing turbulent flow in a circular-sectioned 180° bend has been investigated. The bend had a radius of 104 mm and a curvature radius ratio of 4.0 with long, straight upstream and downstream pipes. Measurements of the longitudinal, radial and circumferential components of mean velocity, and corresponding components of the Reynolds stress were obtained with a hot wire anemometer at a Reynolds number of 6×104 and at various longitudinal stations. The velocity fields of the primary and secondary flows and the Reynolds stresses were illustrated in the form of contour map or vector diagram. Moreover, the mean quantities characterizing the bend flow, i.e., the deflection of the primary flow in the cross section, the intensity of the secondary flow and the turbulence energy, were shown in a graphic form against the longitudinal distances. In the section upstream from a bend angle of about 60°, both the flows through the 180° and the 90° bend are closely similar in their behavior. In the section from the bend angle of 90°, the high-velocity regions, however, occur near the upper and lower walls as a result of strong secondary flow and the turbulence with high level emerges in the central region of the bend. Just behind the bend exit, an additional pair of vortices appears in the outer part of the cross section owing to the transverse pressure difference. In the downstream tangent, the flow returns slowly to the proper flow in a straight pipe, but it needs a longer distance for recovery than in the 90° bend.