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  • 1
    Digitale Medien
    Digitale Medien
    Molecular Cloning of cDNA to mRNA for a Cerebellar Spot 35 Protein (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 48 (1987), S. 0 
    Details
    ISSN: 1471-4159
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Abstract: The nucleotide sequence of mRNA for rat cerebellar spot 35 protein, a Ca-binding protein, was determined from recombinant complementary DNA (cDNA) clones. The sequence was composed of 1, 714 base pairs (bp) which included the 783 bp of the complete coding region, the 130 bp of the 5′-noncoding region, and the 801 bp of the 3-noncoding region containing a polyadenylation signal. In addition, a polyadenylic acid [poly(A)] tail was also found. Because the size of spot 35 mRNA was estimated to be about 1,900 bases by Northern blot analysis, the longest insert was verified to contain a nearly full-length cDNA sequence including the poly(A) tail. The amino acid sequence of the protein deduced from the nucleotide sequence contains 261 amino acids and at least five Ca-binding domains. There was a high homology in the amino acid sequences (79%) and the nucleotide sequences (77%) between spot 35 protein and chick intestinal Ca-binding protein (28K).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1471-4159.1987.tb05706.x
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  • 2
    Digitale Medien
    Digitale Medien
    Fabrication of transparent iron-oxide films by means of sol-gel synthesis (abstract) (1988)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 64 (1988), S. 5452-5452 
    Details
    ISSN: 1089-7550
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: Transparent iron-oxide films were fabricated by sol-gel synthesis using a solution of ferric nitrate (III) dissolved in ethylene glycol.1 The solution was kept at 80 °C and stirred constantly in a nitrogen atmosphere. The gel with an appropriate viscosity was spin coated on soft glass plates, then dried and heated in air at various temperatures for 5 h. The films thus prepared are about 0.2 μm thick, amber colored, and especially transparent in the near-infrared region, whose transmittance exceeds 90%. Maximum saturation magnetization 4πMs=0.74 kG [curve (a)] was obtained by annealing at 450 °C, which is still insufficient to use practical application. Reduction heat treatment in a hydrogen atmosphere is found to be very effective to improve magnetic properties: 4πMs is increased to 3 kG [curve (b)], which is about four times as large as the previous one, but the films become semitransparent due to formation of magnetite particles. Original high transmittance recovers by successive annealing in air at 400 °C without any degradation of magnetic properties [curve (c)], where diffraction peaks of maghemite were observed. This strongly suggests that magnetic anisotropy may be arbitrarily controlled by forming linear chains of ferromagnetic particle clusters through reduction heat treatment in a magnetic field. Faraday rotation θF of the samples was also measured. These films are promising as a new type magneto-optic material.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.342345
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  • 3
    Digitale Medien
    Digitale Medien
    A highly efficient neutron time-of-flight detector for inertial confinement fusion experiments : Proceedings of the 12th topical conference on high temperature plasma diagnostics (1999)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 70 (1999), S. 1221-1224 
    Details
    ISSN: 1089-7623
    Quelle: AIP Digital Archive
    Thema: Physik , Elektrotechnik, Elektronik, Nachrichtentechnik
    Notizen: We have developed the highly efficient neutron detector system MANDALA for the inertial-confinement-fusion experiment. The MANDALA system consists of 842 elements plastic scintillation detectors and data acquisition electronics. The detection level is the yield of 1.2×105 for 2.5 MeV and 1×105 for 14.1 MeV neutrons (with 100 detected hits). We have calibrated the intrinsic detection efficiencies of the detector elements using a neutron generator facility. Timing calibration and integrity test of the system were also carried out with a 60Co γ ray source. MANDALA system was applied to the implosion experiments at the GEKKO XII laser facility. The integrity test was carried out by implosion experiments. © 1999 American Institute of Physics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.1149430
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  • 4
    Digitale Medien
    Digitale Medien
    Magneto-optical properties of Bi-substituted epitaxial LuSmIG films (1985)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 57 (1985), S. 3882-3884 
    Details
    ISSN: 1089-7550
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: Magneto-optical, compositional, and magnetic properties of Biz(LuSmPb)3−zFe5O12 (z=0.05–0.9) grown epitaxially on Nd3Ga5O12 substrates from melts with different PbO/Bi2O3 ratios were studied. Chemical analysis by an x-ray microanalyzer indicates that Bi is incorporated into these films primarily by replacing Sm. Room-temperature Faraday rotation θF was measured at a wavelength of 633 nm. θF is proportional to Bi concentration z in the film and is −2×104 deg/cm for z=1.0. θF of Ga-substituted films for a Ga concentration of 0.6 was about 20% smaller than that of the unsubstituted film. The magnetic uniaxial anisotropy field Hk measured by a vibrating sample magnetometer ranged from 2500 to 5400 Oe. From the analysis of Hk data, it was found that Pb-Lu and Bi-Lu pairs were major contributors to Hk of the present garnet films.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.334904
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  • 5
    Digitale Medien
    Digitale Medien
    Numerical Liouville approach: Formulation of third-order nonlinear optical susceptibilities (1995)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 102 (1995), S. 2986-2995 
    Details
    ISSN: 1089-7690
    Quelle: AIP Digital Archive
    Thema: Physik , Chemie und Pharmazie
    Notizen: As a continuation of a previous paper [Phys. Rev. A 50, 2989 (1994)], this article develops and describes in detail the new nonperturbative approach to the third-order nonlinear optical susceptibilities χ(3)g(ω) in third-harmonic generation (THG), electric-field-induced second-harmonic generation (EFISH), degenerate four-wave mixing (DFWM), electric-field-induced optical rectification (EFIOR), and electric-field-induced Kerr effect (EFIKE) for N-state quantum systems interacting with intense electric fields. The present method utilizes the Fourier transformation of numerically exact solutions of the Liouville equation, so that it can provide both real and imaginary nonlinear optical spectra valid for arbitrary laser intensities, frequencies, and relaxation. As an application of the method, we investigate the characteristics of nonlinear optical spectra in THG, EFISH, and DFWM for a three-state model that mimics the electronic excited states of t-octatetraene obtained from a full configuration interaction (FCI) calculation using the Pariser–Parr–Pople (PPP) Hamiltonian. Comparisons between the present nonperturbative and the conventional perturbative results are also carried out. The magnitudes of the third-order nonlinear susceptibilities in the off-resonant region show the following trend: χ(3)(THG)(approximately-greater-than)χ(3)(EFISH)(approximately-greater-than)χ(3)(DFWM), with χ(3)(THG) exhibiting the largest frequency dispersion. © 1995 American Institute of Physics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.468607
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  • 6
    Digitale Medien
    Digitale Medien
    Numerical Liouville approach: Three-type analysis of virtual excitation processes of third-order nonlinear optical spectra in third-harmonic generation (1995)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 102 (1995), S. 2996-3004 
    Details
    ISSN: 1089-7690
    Quelle: AIP Digital Archive
    Thema: Physik , Chemie und Pharmazie
    Notizen: An analysis method based on virtual excitation processes is developed in numerical Liouville approach, which is a new nonperturbative method presented before. In this analysis method (NLA three-type analysis method), third-order nonlinear optical susceptibilities χ(3)g(ω) obtained by the NLA method can be divided into the three-type virtual excitation processes similar to the three-type analysis based on the time-dependent perturbation theory (TDPT three-type analysis). The NLA three-type analysis method is superior to the TDPT three-type analysis one in the points as follows: (1) the NLA three-type analysis can treat the phenomena caused by the intensity-dependent nonlinear susceptibilities including nonperturbative effects and (2) the NLA three-type analysis can divide the resonant nonlinear susceptibilities into three types of virtual excitation processes. In order to explain the procedure of the present analysis, we try to elucidate the characteristics of virtual excitation processes of nonlinear optical spectra in THG for a three-state model that mimics the electronic excited states of a system with noncentrosymmetric charge distribution. © 1995 American Institute of Physics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.468608
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  • 7
    Digitale Medien
    Digitale Medien
    Two cases of adult T-cell leukaemia (1986)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 114 (1986), S. 0 
    Details
    ISSN: 1365-2133
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: A 70-year-old male and a 46-year-old female, both living in Japan, were seen because of multiple erythematous papules and plaques on the trunk and extremities. The peripheral white blood cell counts were increased to 30000–80000/mm3. The majority of white cells had characteristic lobulated and deeply indented nuclei. The patients and most of their family members had anti-ATLV antibody. Using an OKT series of monoclonal antibodies the tumour cells were found to be T-cells. Trisomy 21 was detected in the first case and ATL virus proviral DNA in the second.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2133.1986.tb02858.x
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  • 8
    Digitale Medien
    Digitale Medien
    Expression of Immediate Early Genes and Vasopressin Heteronuclear RNA in the Paraventricular and Supraoptic Nuclei of Rats After Acute Osmotic Stimulus (2005)
    Oxford, UK : Blackwell Science Ltd
    Journal of neuroendocrinology 17 (2005), S. 0 
    Details
    ISSN: 1365-2826
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Monitoring the expression of immediate early genes (IEGs) is useful for following stress-induced cellular responses in the neuroendocrine system. We have examined the transcriptional activities of four IEGs (c-fos, junB, NGFI-A and NGFI-B) and of the arginine vasopressin (AVP) gene in the hypothalamic paraventicular (PVN) and supraoptic nuclei (SON) of rats after acute osmotic stimuli, using in situ hybridization histochemistry. After intraperitoneal (i.p.) administration of hypertonic saline (2% body weight, 900 mOsm/kg), the expression levels of all IEG mRNAs were increased significantly both in the PVN and SON at as early as 10 min, peaked at 30 min and remained elevated until 60 min. The expression of AVP heteronuclear (hn)RNA also peaked at 30 min, and remained elevated until 180 min. Thirty min after i.p. administration of hypertonic saline (600 mOsm/kg), the expression levels of all IEG mRNAs in the PVN and SON were significantly increased in comparison with those after i.p. administration of isotonic saline (290 mOsm/kg). Regression analysis revealed that expression levels of the IEG mRNAs and AVP hnRNA were positively correlated with the plasma concentration of sodium, and the rates of increase of the expression levels of all IEG mRNAs were similar. The expression levels of all IEG mRNAs examined are useful markers for following the changes of the AVP gene transcription in the PVN and SON after acute osmotic stimuli in rats.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2826.2005.01297.x
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  • 9
    Digitale Medien
    Digitale Medien
    Spontaneous hybridization in the laboratory and genetic markers for the identification of hybrids between two atherinid species, Odontesthes bonariensis (Valenciennes, 1835) and Patagonina hatcheri (Eigenmann, 1909) (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 28 (1997), S. 0 
    Details
    ISSN: 1365-2109
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: This study reports the occurrence of spontaneous hybridization between Odontesthes bonariensis (Valenciennes, 1835) and Patagonina hatcheri (Eigenmann, 1909), two South American freshwater atherinids, while adults of both species were being held in a communal laboratory tank, and describes suitable genetic markers for distinction of both species and their hybrids. Combined allozyme and mitochondrial DNA restriction length polymorphism analyses of offspring from three spawnings in the communal tanks confirmed the presence of hybrids along with purebreds in each spawning. Hybrids of an O. bonariensis mother X a P. hatcheri father were produced along purebred O. bonariensis in one occasion, whereas hybrids of P. hatcheri mothers X O. bonariensis fathers were produced along purebred P. hatcheri in two occasions. Three enzyme systems (coded by loci LDH-B*, PGM*, and CK*) out of 15 (20 loci) screened allowed distinction of species and their hybrids, but the usefulness of LDH-B* for field work may be limited by intraspecific polymorphism. Eight restriction enzymes (Apa I, Bgl II, Eco RI, Eco RV, Eco T22I, Hinc II, Hind III, and Pvu II) among 11 produced diagnostic restriction fragments applicable for species (motherhood) distinction. The relevance of these findings for the management of natural genetic resources and the aquaculture of these two species is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2109.1997.tb01045.x
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  • 10
    Digitale Medien
    Digitale Medien
    Cytokine production in children outgrowing hen egg allergy (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 26 (1996), S. 0 
    Details
    ISSN: 1365-2222
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Background Approximately 40 to 80% of egg-allergic children outgrow egg allergy after 2 to 5 years.Objective and methods To detail the immunologic mechanisms involved in the development of tolerance to egg proteins, the bahince between interleukin 4 (IL4) and interferon-γ (IFN-γ) synthesis in patients with active atopic dermatitis allergic to hen egg and in those outgrowing hen egg allergy was evaluated.Results A marked increase in IL4 and a decrease in IFN-γ synthesis by peripheral blood lymphocytes following ovalbumin (OVA) specific in vitro stimulation was observed in active atopic dermatitis. In contrast, OVA-induced IL4 synthesis in patients in remission was comparable to that in normal individuals. An intriguing finding was higher production of IFN-γ by lymphocytes from ovalbumin-inscnsitive patients in remission as compared to normal individuals following antigen stimulation. although cell proliferation in OVA-stimulated lymphocytes was reduced in patients during remission.Conclusion OVA antigen may be capable of inducing a population of Thl-type cells to produce cytokines such as IFN-γ, resulting in suppression of Th2-type responses, i.e. IL4 secretion. We speculate that the changes in the balance of relevant antigen-induced cytokine synthesis seen in such patients may be causally associated with the improvement in their clinical status.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2222.1996.tb00527.x
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