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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of the antibody response in oesophageal cicatricial pemphigoid (1999)
    Oxford BSL : Blackwell Science Ltd
    British journal of dermatology 140 (1999), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Cicatricial pemphigoid (CP) is a subepidermal, autoimmune bullous dermatosis. It is classified as a clinical subset of bullous pemphigoid (BP). However, it differs from BP in some significant ways: (i) in CP mucosal involvement with clinical scarring is prominent; (ii) there is a prominent IgA class antibody response alone or in addition to the IgG class antibody response; and (iii) there is a heterogeneous antibody response in CP, whereas in BP the majority of the antibodies are directed against a 180-kDa hemidesmosomal protein, bullous pemphigoid antigen 2 (BPAg2). Oesophageal involvement in CP is a rare, but often devastating manifestation. In this study we examined the humoral autoimmune response in oesophageal CP, in an attempt to characterize the autoantibody reactivity profile. We used direct and indirect immunofluorescence and Western immunoblotting using normal human skin and oesophagus substrates. We studied patient sera over time in order to search for evidence of epitope spreading in these patients. All patients had positive direct immunofluorescence of perilesional oesophageal epithelium. All patients had positive circulating antibasement membrane zone autoantibody titres. There was a significant IgA class in addition to an IgG class autoantibody response. IgA and IgG antibodies demonstrated significant reactivity with BPAg2 and the 97 kDa linear IgA disease antigen on Western immunoblot suggesting intraprotein epitope spreading. There was no evidence of interprotein epitope spreading over time. Our findings suggest that there is a heterogeneous antibody response in oesophageal CP with the predominant antigen being BPAg2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.1999.02816.x
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  • 2
    Electronic Resource
    Electronic Resource
    Authors' reply (1989)
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 96 (1989), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.1989.tb03371.x
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  • 3
    Electronic Resource
    Electronic Resource
    Secular trend in the rate of small-for-gestational-age infants: Haguenau Study 1971-1985 (1988)
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 95 (1988), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary. Between 1971 and 1985, at the Haguenau Maternity Hospital, a 20% decrease was observed in the rate of small-for-gestationalage infants (birthweight 〈10th centile using figures from all the study births) among 20 101 births between 28 and 42 weeks. This decrease could not be explained by simultaneous changes in socio-demographic and anthropometric characteristics of the pregnant women, nor a recruitment bias. The earliness of prenatal care at the Maternity Hospital may have played a role in the downward trend. Further research is needed to confirm the influence of prenatal care on the rate of small-forgestational-age babies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.1988.tb06815.x
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of c-fos in cultured human nevus cells: an increase over melanocytes and melanoma cells (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Experimental dermatology 6 (1997), S. 0 
    Details
    ISSN: 1600-0625
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract Nevus cells exhibit growth characteristics in culture which differentiate them from melanocytes and melanoma cells. We examined the expression of c-jun, c-fos and jun-B mRNA levels in cultures of different melanocytic cell types to determine if biologic differences among these cells was due to their level of proto-oncogene expression. Because cell growth and differentiation are also known to be affected by serum conditions, the expression of c-jun, c-fos and jun-B was examined under normal serum conditions and serum starved and repleted conditions which stimulates proto-oncogene expression. Expression of c-jun and jun-B was not significantly different among the cell types studied under normal serum conditions, or serum starved and refed conditions and c-fos was not detectable in any of the unstimulated cell types. In contrast, when the cells were serum starved and refed, the level of c-fos expression was uniformly increased (2-10 fold) in 3 different nevus ceil lines. This increase was not seen in normal melanocyte cultures or 2 melanoma cell lines. With serum deprivation and repletion, c-fos was also elevated in 1 melanoma cell line. We conclude that the regulation of the proto-oncogene c-fos is different in nevus cells than in normal melanocytes, which may contribute to the different growth characteristics seen with nevus cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0625.1997.tb00178.x
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  • 5
    Electronic Resource
    Electronic Resource
    Zum enzymatisch-immunochemischen Nachweis des Haschischkonsums und seiner dünnschichtchromatographischen Absicherung (1985)
    Springer
    International journal of legal medicine 94 (1985), S. 219-225 
    Details
    ISSN: 1437-1596
    Keywords: Cannabinoids, enzyme immunoassay ; THC-metabolite, detection in urine and blood ; Cannabinoide, Enzymimmunoassay ; THC-Metabolit, Nachweis in Urin und Blut
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Mit Hilfe des Enzymimmunoassay (EMIT-dau-Syva) lassen sich Cannabinoide in Blut und Urin nach inhalativer (10 mg THC) oder oraler (30 mg THC) Aufnahme nachweisen. Die Kurvenverläufe werden mitgeteilt. Im Urin waren erhöhte Spiegel bis zu einer Woche nachweisbar. Eine Absicherung der EMIT-Befunde ist mittels Dünnschichtchromatographie nach absorptiver Anreicherung an einer C18-Extraktionssäule möglich. Nachweisgrenze ist 10 ng THC-Carbonsäure/ml bei Einsatz von 10 ml Urin.
    Notes: Summary The EMIT cannabinoid assay was used for screening blood and urine after smoking tetrahydrocannabinol (THC; 10 mg) or ingestion of THC (30 mg). Cannabinoid levels in urine remain detectable up to 1 week. Confirmation was done by adsorption of the THC carboxylic acid onto a C18 extraction column and elution with acetone and TLC. The method is simple and sensitive and is applicable with common laboratory equipment. The detection limit is 10 ng/ml, using 10 ml urine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00204086
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  • 6
    Electronic Resource
    Electronic Resource
    Hydrocoolers: Are They a Mechanism for Pesticide Transfer? (1998)
    Springer
    Bulletin of environmental contamination and toxicology 60 (1998), S. 685-692 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001289900681
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  • 7
    Electronic Resource
    Electronic Resource
    HEMOCO: a French prospective study of hemophiliacs infected by human immunodeficiency virus type 1 (HIV-1) (1997)
    Springer
    Hematology and cell therapy 38 (1997), S. 193-198 
    Details
    ISSN: 1279-8509
    Keywords: Hemophilia ; Human immunodeficiency virus (HIV) ; Cohort ; Age ; AIDS ; Death
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract HEMOCO is a multicenter prospective cohort set up in 1989 to monitor 407 French hemophiliacs infected by HIV-1 and recruited in 4 hemophilia treatment centers in the Paris region. As of 15 July 1995, 42% of the patients in the cohort had developed stage B HIV disease and 29% stage C disease (AIDS); 23.1% of the patients had died. The cumulative proportion of patients with AIDS was 4.5% at 5 years and 27.4% at 10 years, while the respective mortality rates were 3.8% and 19.5%. In our study, only age was predictive of AIDS, with an estimated relative risk of 1.2 per 10-year age increment; this factor was also predictive of death. After 10 years of follow-up, 6.1% of the study population had no clinical or laboratory signs of immunodepression. The follow-up protocol in the HEMOCO protocol is the same as that in the French SEROCO study, which includes men infected by HIV-1 through sexual contact. This will allow us to compare the progression of HIV infection between these two exposure groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s00282-996-0193-7
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  • 8
    Electronic Resource
    Electronic Resource
    Quality assurance in forensic toxicological analysis (1996)
    Springer
    Accreditation and quality assurance 1 (1996), S. 201-206 
    Details
    ISSN: 1432-0517
    Keywords: Key words Forensic science ; Toxicological analysis ; Quality assurance ; Round robins ; Accreditation of forensic laboratories
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract  In this paper, we review the work done in forensic toxicological analysis during about the last 30 years in Europe and to some extent in other parts of the world to assure the quality of toxicological investigations. Guidelines for carrying out toxicological analyses set up by professional bodies in the United States (AAFS/SOFT), Europe (GTFCh) and worldwide (TIAFT) are mentioned, and the problems of complying with the regulations set up in EN 45 000/ISO 9000 are discussed in detail. There are still diverging opinions as to whether toxicological laboratories should comply with good laboratory practice or with EN 45 000, and, as fas as Germany, Switzerland and Austria are concerned, a working group of the GTFCh is discussing which procedure should be adopted. Apart from having this discussion, the group aims to standardize suitable methods and is planning the organization of suitable round robins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007690050068
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  • 9
    Electronic Resource
    Electronic Resource
    Suchtmittelnachweis (1999)
    Springer
    Der Internist 40 (1999), S. 601-604 
    Details
    ISSN: 1432-1289
    Keywords: Schlüsselwörter Drogen ; Nachweis ; Sucht ; Drogennachweis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zum Thema Eine ausgefeilte Analytik vom Schnelltest bis hin zur Gaschromatographie-Massenspektrometrie macht den Drogennachweis unter Umständen noch Monate nach der Einnahme möglich. Bei der Auswahl der Testverfahren sind natürlich auch ökonomische Gesichtspunkte und – je nach Herstellerfirma – unterschiedliche Empfindlichkeiten zu berücksichtigen. Im klinischen, vor allem aber im forensischen Bereich muß der Sicherheit der Ergebnisse von immunologischen Testverfahren besondere Aufmerksamkeit gewidmet werden. Bei deren Anwendung, z.B. bei Amphetaminen, zu denen auch die Ecstasy-Verbindungen zählen, sind möglicherweise falsch-negative Befunde nicht auszuschließen. Auch die Ärztinnen und Ärzte, die mit Drogenabhängigen nur marginalen Kontakt und wenig Erfahrungen haben, sollten informiert sein, welcher Methoden man sich zum Drogennachweis bedient. Darüber wird in dieser Arbeit recht verständlich referiert.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001080050376
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  • 10
    Electronic Resource
    Electronic Resource
    Bradyrhizobium japonicum mutants defective in root-nodule bacteroid development and nitrogen fixation (1986)
    Springer
    Archives of microbiology 144 (1986), S. 355-366 
    Details
    ISSN: 1432-072X
    Keywords: Bradyrhizobium ; Electron microscopy ; Mutants ; Nitrogen fixation ; Nodulation ; Soybean ; Symbiosis ; Transposon Tn5
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genome of the slow-growing Bradyrhizobium japonicum (strain 110) was mutagenized with transposon Tn5. A total of 1623 kanamycin/streptomycin resistant derivatives were screened in soybean infection tests for nodulation (Nod) and symbiotic nitrogen fixation (Fix). In this report we describe 14 strains possessing a stable, reproducible Nod+Fix- phenotype. These strains were also grown under microaerobic culture conditions to test them for free-living nitrogen fixation activity (Nif). In addition to strains having reduced Fix and Nif activities, there were also strains that had reduced symbiotic Fix activity but were Nif+ ex planta. Analysis of the genomic structure revealed that the majority of the strains had a single Tn5 insertion without any further apparent physical alteration. A few strains had additional insertions (by Tn5 or IS50), or a deletion, or had cointegrated part of the vector used for Tn5 mutagenesis. One of the insertions was found in a known nif gene (nifD) whereas all other mutations seem to affect different, hitherto unknown genes or operons. Several mutant strains had an altered nodulation phenotype, inducing numerous, small, widely distributed nodules. Light and electron microscopy revealed that most of these mutants were defective in different stages of bacteroid development and/or bacteroid persistence. The protein patterns of the mutants were inspected by two-dimensional gel electrophoresis after labelling microaerobic cultures with l-(35S)methionine. Of particular interest were mutants lacking a group of proteins the synthesis of which was known to be under oxygen control. Such strains can be regarded as potential regulatory mutants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409885
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