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  • 1995-1999  (2)
  • 1975-1979  (6)
  • 1
    Electronic Resource
    Electronic Resource
    Anomeric preferences ofd-glucose uptake and utilization by cerebral cortex slices of rats (1979)
    Springer
    Neurochemical research 4 (1979), S. 505-516 
    Details
    ISSN: 1573-6903
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract On aerobic incubation of rat cerebral cortex slices with anomers ofd-glucose and with 2-deoxy-d-glucose (2DG) for 5 min, the disappearance of β-d-glucose from the incubation mixture was greater than that of α-d-glucose and both anomers had a greater rate of disappearance than that of 2DG. In addition, there were significantly greater consumption of oxygen and production of lactate with the β-anomer than with the α-anomer. In similar experiments with3H-labeledd-glucose anomers and [1-3H]-3-O-methyl-d-glucose (3MG), the accumulation of [1-3H]-β-d-glucose (up to 5 min) by rat cerebral cortex slices was greater than that of [1-3H]-α-d-glucose. Although initially lower than that of the anomers, the accumulation of [1-3H]-3MG increased at a greater rate and, by 5 min of incubation, was greater than that of both glucose anomers. This preferential accumulation was seen to disappear when the slices were preincubated with 2DG (hexokinase inhibitor) or when the temperature of incubation was reduced to 20°C. Under those conditions the data with the glucose anomers were similar to those obtained with 3MG. Our data then suggested that the greater accumulation of β-d-glucose than of α-d-glucose by the slices was probably not due to differences in transport through brain cell membranes but rather to the preferential metabolism of the β-d-glucose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00964644
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The localization of mutarotase in rat kidney (1979)
    Springer
    Cellular and molecular life sciences 35 (1979), S. 1094-1097 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization of mutarotase in rat kidney was investigated by fluorescein-labelled and peroxidase-labelled antibody techniques, and by method of isolation of the nuclei and cytoplasm in non-aqueous solvents. In these immunohistochemical studies, mutarotase was almost exclusively recognized in the nuclei of epithelial cells of renal tubules and glomeruli in rat. The specific activity of mutarotase was found to be 1.5 times higher in the nuclei (122 units/g dry wt) than that in the cytoplasm (80 units/g dry wt) isolated with non-aqueous solvents. These results suggest that mutarotase may be involved in the metabolism of D-glucose in nuclei.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01949964
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Different effects of D-glucose anomers for respiration of bacterial germinated spores (1979)
    Springer
    Cellular and molecular life sciences 35 (1979), S. 470-472 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Effects of α- or β-D-glucose on the respiration of germinated spores (only germinated spores not including swollen spores and elongated spores) ofBacillus subtilis andB. megaterium were studied. In our conditions, net amount of oxygen consumed by 1010 germinated spores ofB. subtilis per min after addition of α- or β-D-glucose was 1.6 μg or 6.6 μg (β/α=4.13), while that byB. megaterium was 4.5 μg or 6.8 μg (β/α=1.51), respectively. However, the net amounts of oxygen consumed by 1010 vegetative cells per min after addition of α- or β-D-glucose were identical, forB. subtilis in both cases 443.0 μg and forB. megaterium in both cases 604.4 μg.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01922712
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  • 4
    Electronic Resource
    Electronic Resource
    Uptake of D-glucose anomers by rat retina (1977)
    Springer
    Cellular and molecular life sciences 33 (1977), S. 19-20 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Uptake ofd-glucose anomers by isolated rat retina was studied. After 3 min incubation at 37°C in the presence of α or β anomer (750 μg/ml), a significantly greater uptake (1.32 mg/g wet tissue) of β-anomer was observed compared with that of α-d-glucose (1.11 mg/g wet tissue). This result and other data suggest that the carrier ford-glucose transport in the retina prefers the β-anomer stereospecifically.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01936727
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  • 5
    Electronic Resource
    Electronic Resource
    Anomeric compositions of D-glucose in tissues and blood of rat (1978)
    Springer
    Cellular and molecular life sciences 34 (1978), S. 167-169 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The anomeric compositions of D-glucose in the liver, kidney, heart, blood and plasma of rat were determined by our method for the assay of D-glucose anomers and the percentages of the β-anomer were found to be 61.8, 61.0, 62.4, 62.7 and 62.9, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01944655
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  • 6
    Electronic Resource
    Electronic Resource
    Tissue and subcellular distribution of glucokinase in rat liver and their changes during fasting-refeeding (1995)
    Springer
    Histochemistry and cell biology 103 (1995), S. 31-38 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The distribution of glucokinase in rat liver under both normal feeding and fasting-refeeding conditions was investigated immunohistochemically. Under normal feeding conditions, glucokinase immunoreactivity was observed in both nuclei and cytoplasm of parenchymal cells. The nuclei were stained intensely and evenly, whereas the cytoplasm showed weak immunoreactivity of different degrees of staining intensity depending on the location of the cells. The cytoplasm of perivenous hepatocytes was stained more intensely, though not so much more, than that of periportal hepatocytes. The cytoplasm of hepatocytes surrounding the terminal hepatic venule (THV), of hepatocytes surrounding the portal triad, and of some other hepatocytes showed a stronger immunoreactivity than that of residual hepatocytes. The nuclear immunoreactivity in hepatocytes surrounding the portal triad and in some other hepatocytes was weak or absent, and positive immunoreactivity was detected at the plasma membrane of some of these cells. After 72 h of fasting, glucokinase immunoreactivity was markedly decreased in all hepatocytes. After the start of refeeding, the cytoplasmic immunoreactivity began to increase first in the parenchymal cells surrounding the THV and extended to those in the intermediate zone followed by those in the periportal zone. In contrast, the increase in nuclear immunoreactivity started in hepatocytes situated in the intermediate zone adjacent to the perivenous zone and then extended to those in the perivenous zone followed by those in the periportal zone. Hepatocytes surrounding either THV or portal triad showed a distinctive change in immunoreactivity during the refeeding period. After 10 h of refeeding, strong immunoreactivity was observed in both the cytoplasm and the nuclei of all hepatocytes, and appreciable glucokinase immunoreactivity was detected at the plasma membrane of some hepatocytes. These findings are discussed from the standpoint of a functional role of glucokinase in hepatic glucose metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01464473
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  • 7
    Electronic Resource
    Electronic Resource
    Perioperative tumor localization for laparoscopic colorectal surgery (1997)
    Springer
    Surgical endoscopy and other interventional techniques 11 (1997), S. 1013-1016 
    Details
    ISSN: 1432-2218
    Keywords: Key words: Tumor localization — Laparoscopic surgery — Colon tumors — Rectal tumors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Because of the inability to palpate colonic tumors during laparoscopy, their location must be precisely identified before resection is undertaken. Method: A retrospective study was performed of 58 patients in order to be able to describe our methods of tumor localization for laparoscopic colorectal operations and to review their effectiveness. Results: In all patients, the entire colon was examined preoperatively by colonoscopy. In one patient, preoperative colonoscopic localization was inaccurate. In 31 patients, tumors were easily detectable at surgery. In five patients with the tumor in the right colon, even though the lesion was not detectable at surgery, right colectomy was performed without marking because preoperative colonoscopy reliably identified the lesion adjacent to the ileocecal valve. Twenty-two patients required some type of procedure to localize the tumor. The procedures and their problems were as follows: preoperative tattoo (five)—tattoo not visualized (one); intraoperative colonoscopy alone (six), combined with intraoperative tattoo (four) or clip (three)—poor operative exposure due to bowel distension (nine), hard to see the clip (three), dislodged clip (two), inadequate resection margin (one); intraoperative proctoscopy alone (two), combined with laparoscopic stitch (two)—no problems. In no patient was tumor present at a resection line and in no patient was the wrong segment resected. Conclusions: Reliable preoperative identification of the tumor adjacent to the ileocecal valve can permit right colectomy without marking. Lesions in the upper rectum can be approached via intraoperative proctoscopy ± suture placement. If the surgeon anticipates intraoperative localization may be difficult, lesions other than rectal or cecal ones should probably be marked by preoperative tattooing. Further studies regarding the technique of tattooing are warranted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004649900514
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  • 8
    Electronic Resource
    Electronic Resource
    Multiple forms of human kidney mutarotase (1978)
    Springer
    Cellular and molecular life sciences 34 (1978), S. 563-564 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 4 forms of mutarotase from human kidney were demonstrated by DEAE-cellulose column chromatography. A major form of them was purified to homogeneity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01936959
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