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  • 1
    Electronic Resource
    Electronic Resource
    The long-term cytoskeletal rearrangement induced by rabbit enteropathogenic Escherichia coli is Esp dependent but intimin independent (1999)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 31 (1999), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Attaching and effacing rabbit enteropathogenic Escherichia coli (REPEC) of the O103 serogroup adhere diffusely on HeLa cells and trigger a slow progressive cytopathic effect (CPE) characterized by the recruitment of vinculin and the assembly of actin stress fibres. In contrast to REPEC O103, the reference human EPEC strain E2348/69 is unable to trigger the CPE. In this study, we have shown first that the fimbrial adhesin AF/R2, which mediates the diffuse adhesion of REPEC O103, was not sufficient to induce the CPE capability upon E2348/69. Non-polar mutants of REPEC O103 for espA, espB, espD and eae were then constructed. The four mutants were unable to induce attaching and effacing lesions in the rabbit ileal loop model. The esp mutants were no longer able to induce the CPE, whereas the eae mutant still induced the CPE. Each espA, -B, -D mutant could be fully complemented in trans by the corresponding cloned esp genes from both the parental strain and the CPE-negative E2348/69 strain, indicating that no single esp encodes the information needed to confer the CPE phenotype. In conclusion, the CPE is the first example of an Esp-dependent but Eae (intimin)-independent alteration of the host cell cytoskeleton by certain EPEC strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1999.01138.x
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  • 2
    Electronic Resource
    Electronic Resource
    A transferred NOE study of a tricyclic analog of acyclovir bound to thymidine kinase (1996)
    Springer
    Journal of biomolecular NMR 8 (1996), S. 261-272 
    Details
    ISSN: 1573-5001
    Keywords: Acyclovir analogs ; Thymidine kinase ; Transferred NOE ; Relaxation matrix
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary A purine derivative with an acyclic sugar analog, 3,9-dihydro-3-[(2-hydroxyethoxy)methyl]-6-ethyl-9-oxo-5H-imidazo[1,2-a]purine, was studied in the free state and in complex with herpes simplex virus thymidine kinase (HSV1 TK). Transferred NOE experiments, combined with a full relaxation matrix analysis of the substrate's spin system, resulted in a set of distance constraints for all proton pairs. These constraints were used in structure determination procedures based on simulated annealing and molecular dynamics simulations to obtain a family of structures compatible with the experimental NMR data. The results indicate that, although in both states the chains have the syn orientation with respect to the aromatic rings, in the free state the substrate's acyclic moiety is relatively disordered, while in the bound state only one specific conformation is preferred. Fluctuations can only be seen in the case of the terminal hydroxyl group, for which no NOE was recorded and hence no constraints were available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410325
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  • 3
    Electronic Resource
    Electronic Resource
    Studies on the Topography of Biomembranes: Regioselective Photolabelling in Vesicles with the Tandem Use of Cholesterol and a Photoactivable Transmembrane Phospholipidic Probe (1996)
    Weinheim : Wiley-Blackwell
    Chemistry - A European Journal 2 (1996), S. 129-138 
    Details
    ISSN: 0947-6539
    Keywords: bilayers ; cholesterol ; membrane ; probes ; phospholipids ; photolabelling ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 2H NMR, DSC and UV studies of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) vesicles have demonstrated the favourable physicochemical properties of the transmembrane phospholipid probes 1 a and 1 b for membrane topographical studies. In particular, in the presence of a physiological amount of cholesterol, only one transmembrane conformation is observed. The use of 1 a and cholesterol together for photolabelling experiments in DMPC vesicles led to a remarkable improvement in the regioselectivity of cross-linking between 1 a and DMPC, and between 1 a and cholesterol: the myristoyl chains functionalized at C11, C12 and C13 made up 95% of the total photolabelled myristates, and cholesterol was principally functionalized at the C25 position on the side-chain. This indicates the formation of a highly ordered bilayer structure and proves directly the orientation of cholesterol perpendicular to the membrane plane with its chain terminal buried in the middle of the bilayer.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/chem.19960020204
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