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  • 1
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Journal of texture studies 30 (1999), S. 0 
    ISSN: 1745-4603
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Nineteen food materials were pulverized and passed through standard sieves to prepare samples with different particle sizes (23–500 μm). Each sample was tested for physical properties, and sensory evaluation tests were conducted to obtain the graininess threshold value and the degree of graininess discrimination. The graininess threshold value tended to decrease and the degree of graininess discrimination tended to increase with increasing degree of circularity, with decreasing solubility, with decreasing water absorption rate- particle size coefficient, and with increasing WK2/K1. The degree of graininess discrimination tended to increase with increasing particle size and increasing WK2. With a multiple-regression analysis, the graininess threshold value was expressed by six physical properties, and the degree of graininess discrimination was expressed by eight physical properties, each with high contribution rates.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Journal of texture studies 28 (1997), S. 0 
    ISSN: 1745-4603
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Microcrystalline cellulose was dispersed as a gritty substance in an emulsion, and sensory evaluation was conducted to examine the effect of the presence of oil on grittiness felt in the mouth. Samples were prepared with three types of microcrystalline cellulose of average particle size 14, 38, and 76 μm and with a concentration in water of 0.03–2.7%, with three types of emulsion with an oil-volume fraction of 0.2–0.7, and with three rates of homogenization of 500–15,000 rpm. The flow behavior and loss modulus of each sample were measured and sensory evaluation was made of the perceived grittiness. A multiple regression analysis of the data shows that the proportion of people who could perceive grittiness was influenced by the following factors and in that order: concentration of microcrystalline cellulose, oil droplet size, loss modulus, and particle size of microcrystalline cellulose. The proportion of people who perceived grittiness also increased with increasing oil droplet size. This was conceivably caused by the uneven distribution of microcrystalline cellulose particles promoted by large oil droplets. An equation was developed relating the proportion of people perceiving grittiness to the physical properties of the sample. The result indicates that factors effecting grittiness perception are identical for emulsions, aqueous suspensions, viscous suspensions and gels, studied previously.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 1438-2199
    Schlagwort(e): Amino acids ; Tryptophanase ; D-tryptophan ; Specificity to optical isomer ; Diammoniumhydrogen phosphate
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Tryptophanase is and is perfectly inert to D-tryptophan under ordinary conditions. However, activity that can degrade D-tryptophan into indole is observed when tryptophanase is in highly concentrated diammoniumhydrogen phosphate solution. The reaction has been so far unknown in tryptophanase metabolic pathways. Here we report the characteristic of the reaction. We also discuss its significance in relation to selection of an amino acid optical isomer from a racemic mixture.
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Amino acids 12 (1997), S. 379-383 
    ISSN: 1438-2199
    Schlagwort(e): Amino acids ; Tryptophanase ; D-tryptophan ; Diammonium-hydrogen phosphate ; Kinetic analysis ; Stereospecificity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Tryptophanase, which has the very strict stereospecificity to L-tryptophan under ordinary condition, becomes active to D-tryptophan in highly concentrated diammoniumhydrogen phosphate solution. The reaction process of D-tryptophan degradation is studied in terms of kinetics. Diammoniumhydrogen phosphate acts on tryptophanase as activator below 3.1 M, and as noncompetitive inhibitor over it. Additionally, the pathway of the reaction is provided on the basis of kinetic parameters.
    Materialart: Digitale Medien
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  • 5
    ISSN: 1432-0428
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 6
    ISSN: 1432-1793
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Two picophytoplankters,Prochlorococcus marinus andSynechococcus sp., were isolated from the bottom of the euphotic zone (150 m depth) in the western Pacifie Ocean. The concentration ofP. marinus at this depth was more than 104 cells ml−1 while that ofSynechococcus sp. was less than 102 cells ml−1. TheP. marinus isolate has a high divinyl-chlorophylla:b ratio similar to that of the Mediterranean strain, while theSynechococcus sp. isolate is of the phycourobilinrich type. The growth rate ofP. marinus was higher thanSynechococcus sp. when both were cultured under weak blue-green to blue-violet light (ca. 2 μE m−2 s−1). While the chlorophyll-specific absorption spectra showed higher values inSynechococcus sp., the photosynthetic action spectre revealed thatP. marinus was able to use blue-violet light, whereasSynechococcus sp. was able to use blue-green light, more efficiently for photosynthesis. The photosynthetic quantum yield ofP. marinus was higher than that ofSynechococcus sp. at any wavelength between 400 and 700 nm. The calculated in situ photosynthesis rates per Gell volume forP. marinus were estimated to be higher than forSynechococcus sp. at 50 and 150 m depth. These results indicate thatP. marinus photosynthetically surpassesSynechococcus sp. in the blue-light-rieh environment of the oceanic euphotic zone. This may be why the former predominates at depths in temperate to tropical open ocean waters.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Journal of oceanography 51 (1995), S. 289-300 
    ISSN: 1573-868X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geologie und Paläontologie
    Notizen: Abstract Seasonal appearance ofProchlorococcus was studied by flow cytometry in Suruga Bay, Japan in 1992–1993.Prochlorococcus cells were in high concentrations (〉1×104 cells ml−1) from July to October 1992 and September 1993, when the water temperature was over 20°C. The 16S rRNA of the isolated cells showed 98.5% sequence homology with that ofP. marinus (Sargasso strain), indicating that they are the same species. The former has a high divinyl-chlorophyll (DV-Chl.)a/b ratio similar to the Mediterranean strain and different from the Sargasso strain. Maximum concentration ofProchlorococcus at the surface water was 2.5×104 cells ml−1 in August 1992 and their DV-Chl.a accounted for 4.0% of the total chlorophylla. A decrease in cell density to less than 5×103 cells ml−1 was observed from December to May with an exceptional rise in January 1993. WhileProchlorococcus showed a maximum concentration of 3.6×104 cells ml−1 at 10 m depth in September 1992, phycoerythrin (PE)-richSynechococcus spp. were dominant with their maximum concentration of 2.2×105 cells ml−1 in the same water body. On the other hand, phycocyanin (PC)-richSynechococcus spp. and the larger phytoplankters showed maximum concentrations in the surface waters in May and June. BothProchlorococcus and PE-richSynechococcus showed their lowest concentrations in April. A significant positive correlation was obtained between cell concentrations of the PE-richSynechococcus andProchlorococcus.
    Materialart: Digitale Medien
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  • 8
    ISSN: 1432-136X
    Schlagwort(e): Key words Melanophore ; Mastoparan ; Compound 48/80 ; GTP-binding protein ; Goldfish ; Carassius auratus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract  To investigate the functions of GTP-binding protein(s) in the melanosome-aggregating response in fish melanophores, the effects of activators of G-proteins, namely, mastoparan and compound 48/80, were examined in cultured melanophores of the balck-moor goldfish, Carassius auratus. Both mastoparan and compound 48/80 induced an approximately 40% increase in the GTP-hydrolyzing activity in the melanophore membranes compared to the basal level. In intact melanophores, these compounds inhibited the effect of 3-isobutyl-1-methylxanthine, which induced the accumulation of intracellular cAMP. Pretreatment of melanophores with pertussis toxin at 1 μg ⋅ ml-1 for 15 h attenuated the inhibitory effect of mastoparan on the accumulation of cAMP. However, pretreatment with the toxin only slightly attenuated the inhibitory effect of compound 48/80 on the accumulation of cAMP. In addition, compound 48/80 at 1 mg ⋅ ml-1 induced full aggregation of the melanosomes in melanophores, though mastoparan at 5 μmol ⋅ l-1 induced only 10–20% aggregation of melanophores. These results suggest that mastoparan and compound 48/80 can each activate the inhibitory G-protein in goldfish melanophores, which results in inhibition of adenylate cyclase activity. This signal-transduction pathway is involved in the aggregation of melanosomes in these cells.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 9
    ISSN: 1432-136X
    Schlagwort(e): Melanophore ; Mastoparan ; Compound 48/80 ; GTP-binding protein ; Goldfish,Carassius auratus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract To investigate the functions of GTP-binding protein(s) in the melanosome-aggregating response in fish melanophores, the effects of activators of G-proteins, namely, mastoparan and compound 48/80, were examined in cultured melanophores of the balck-moor goldfish,Carassius auratus. Both mastoparan and compound 48/80 induced an approximately 40% increase in the GTP-hydrolyzing activity in the melanophore membranes compared to the basal level. In intact melanophores, these compounds inhibited the effect of 3-isobutyl-1-methylxanthine, which induced the accumulation of intracellular cAMP. Pretreatment of melanophores with pertussis toxin at 1 μg·ml-1 for 15 h attenuated the inhibitory effect of mastoparan on the accumulation of cAMP. However, pretreatment with the toxin only slightly attenuated the inhibitory effect of compound 48/80 on the accumulation of cAMP. In addition, compound 48/80 at 1 mg·ml-1 induced full aggregation of the melanosomes in melanophores, though mastoparan at 5 μmol·l-1 induced only 10–20% aggregation of melanophores. These results suggest that mastoparan and compound 48/80 can each activate the inhibitory G-protein in goldfish melanophores, which results in inhibition of adenylate cyclase activity. This signal-transduction pathway is involved in the aggregation of melanosomes in these cells.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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