Bibliothek

X
feed icon rss

Ihre E-Mail wurde erfolgreich gesendet. Bitte prüfen Sie Ihren Maileingang.

Leider ist ein Fehler beim E-Mail-Versand aufgetreten. Bitte versuchen Sie es erneut.

Vorgang fortführen?

Exportieren
  • 1
    Digitale Medien
    Digitale Medien
    Expression of Heme Oxygenase Isozyme mRNAs in the Human Brain and Induction of Heme Oxygenase-1 by Nitric Oxide Donors (1996)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 67 (1996), S. 0 
    Details
    ISSN: 1471-4159
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Abstract: Heme oxygenase is an essential enzyme in the heme catabolism that produces carbon monoxide (CO). This study was designed to examine the expression of two heme oxygenase isozyme mRNAs in the human brain and to explore the involvement of nitric oxide (NO) and various neuropeptides in the regulation of their expression. Northern blot analysis showed the expression of heme oxygenase-1 and heme oxygenase-2 mRNAs in every region of the brain examined, with the highest levels found in the frontal cortex, temporal cortex, occipital cortex, and hypothalamus. In a human glioblastoma cell line, T98G, treatment with any of three types of NO donors—sodium nitroprusside, 3-morpholinosydnonimine, and S-nitroso-l-glutathione—caused a significant increase in the levels of heme oxygenase-1 mRNA but not in the levels of heme oxygenase-2 and heat-shock protein 70 mRNAs. Sodium nitroprusside increased the levels of heme oxygenase-1 protein but not the levels of heat-shock protein 70 in T98G cells. The increase in content of heme oxygenase-1 mRNA caused by sodium nitroprusside was completely abolished by the treatment with actinomycin D. On the other hand, the levels of heme oxygenase isozyme mRNAs were not noticeably changed in T98G cells following the treatment with 8-bromo cyclic GMP, sodium nitrite, or various neuropeptides, such as calcitonin gene-related peptide, endothelin-1, and corticotropin-releasing hormone. The present study has shown the expression profiles of heme oxygenase-1 and -2 mRNAs in the human brain and the induction of heme oxygenase-1 mRNA caused by NO donors in T98G cells. These findings raise a possibility that the CO/heme oxygenase system may function in concert with the NO/NO synthase system in the brain.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1471-4159.1996.67020482.x
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 2
    Digitale Medien
    Digitale Medien
    A Functioning Composite ‘Corticotroph’ Pituitary Adenoma with Interspersed Adrenocortical Cells (1999)
    Springer
    Pituitary 1 (1999), S. 279-284 
    Details
    ISSN: 1573-7403
    Schlagwort(e): corticotroph ; adrenocortical cells ; immunohistochemistry ; electron microscopy ; pituitary adenoma ; Cushing's syndrome
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We report the third case of a composite corticotroph pituitary adenoma with interspersed adrenocortical cells. The 16-year-old male patient presented with findings of delayed growth and pubertal arrest. In contrast to the previous two cases, this patient's tumor showed evidence of function as demonstrated by an elevated urinary cortisol level. Imaging studies revealed a sellar mass that was excised transsphenoidally. Histologic examination revealed a composite tumor composed of distinct populations of large and small cells. The small cell population was PAS-positive and immunohistochemically positive for adrenocorticotrophic hormone. The large cell population had abundant vacuolated cytoplasm, was negative for PAS and adrenocorticotrophic hormone, and stained positively for a panel of markers found in steroid-producing adrenocortical cells. Both populations showed evidence of proliferation as manifest by the presence of MIB-1 positive cells. Ultrastructural examination confirmed the presence of distinct populations of large adrenocortical cells and small corticotrophs, with intercellular junctions between the 2 cell types. The intimate relationship between the 2 cell population and the activated appearance of the adrenocortical cells suggests the possibility of a paracrine relationship between the two cell types. The identification of 3 patients with sellar tumors demonstrating strikingly similar morphological and ultrastructural features, and all occurring in the second decade of life, suggests that this represents a distinct pathologic entity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009962627216
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 3
    Digitale Medien
    Digitale Medien
    Immunolocalization of aromatase in human breast disorders using different antibodies (1998)
    Springer
    Breast cancer research and treatment 49 (1998), S. S79 
    Details
    ISSN: 1573-7217
    Schlagwort(e): breast ; carcinoma ; aromatase ; immunohistochemistry ; antibody
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Immunolocalization of aromatase can provide important information about the status of intratumoral aromatase of human breast. We have performed immunohistochemistry of aromatase in surgical pathology specimens of human breast disorders using monoclonal and polyclonal antibodies. Both antibodies yielded satisfactory results in immunostaining of aromatase in normal placenta and normal cycling human ovaries. In human breast disorders, immunostaining or aromatase using monoclonal antibodies can only demonstrate immunoreactivity following antigen retrieval. Immunoreactivity can be detected in both carcinoma and stromal cells but nuclear immunoreactivity, which may be represent artefactural changes, can be detected in both normal and neoplastic breasts. Immunostaining using polyclonal antibody revealed aromatase immunoreactivity in cytoplasm of adipocytes, fibroblasts, vascular wall cells and Schwann cells but intense immunoreactivity was detected in adipocytes adjacent to carcinoma invasion in 68/82 carcinoma cases and stromal cells around carcinoma nest in 58/82 cases. Strong aromatase immunoreactivity was detected in 7/69 benign proliferative breast disorder, 5 of which were sclerosing adenosis. Aromatase immunoreactivity was also detected in carcinoma cells in 7/82 cases but aromatase positive carcinoma cells represent less than 5% of tumor cells in all these cases. These results above indicate that polyclonal antibody is considered to be more suitable for aromatase immunohistochemistry in archival materials of breast disorders but further investigations including easy and reproducible scoring system and production of reliable monoclonal antibodies are required to establish aromatase immunohistochemistry as a reliable method of assessing intratumoral aromatase in human breast disorders.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006009128920
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 4
    Digitale Medien
    Digitale Medien
    Immunohistochemical Studies on EGF Family Growth Factors in Normal and Ulcerated Human Gastric Mucosa (1997)
    Springer
    Digestive diseases and sciences 42 (1997), S. 1199-1209 
    Details
    ISSN: 1573-2568
    Schlagwort(e): EPIDERMAL GROWTH FACTOR ; TRANSFORMING GROWTH FACTOR-α ; AMPHIREGULIN ; EPIDERMAL GROWTH FACTOR RECEPTOR ; CRIPTO ; GASTRIC ULCER
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Expression of members of the epidermal growthfactor family, including epidermal growth factor (EGF),transforming growth factor-α (TGF-α),amphiregulin (AR), and Cripto, as well as their putative receptor, epidermal growth factor receptor(EGFR), was studied immunohistochemically in humangastric mucosa to evaluate their possible roles in cellproliferation of normal and regenerative gastric mucosa. We also examined the correlation between cellproliferation and EGFR by double immunohistochemicalstaining for proliferating cell nuclear antigen (PCNA)and EGFR. In normal gastric mucosa, TGF-α, Cripto, and AR immunoreactivities were observed in thesurface epithelial and parietal cells of gastric fundicglands, respectively. EGF immunoreactivity was notobserved in any of normal mucosa examined. EGFR immunoreactivity was detected on foveolar cellsin proliferative zones and in parietal cells. Doubleimmunostaining revealed that EGFR immunoreactivity wasdistributed much more widely than PCNA immunoreactivity. PCNA positive epithelial cells adjacent togastric ulcer margin expressed relatively intense EGFRbut did not express any of the growth factors examined.On the other hand, relatively intense immunoreactivity of both TGF-α and Cripto was detected inPCNA-negative regenerative epithelium located distantfrom gastric ulcer margin. Relative immunoreactivity ofAR in regenerative gastric epithelium associated with ulcer was not different from that innormal gastric mucosa. TGF-α, AR, and Cripto areconsidered to play important roles in normal gastricmucosal proliferation, and TGF-α and Cripto may be involved in ulcer healing, possibly via aparacrine mechanism.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1018897922644
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 5
    Digitale Medien
    Digitale Medien
    Induction of Mineralocorticoid Receptor by Sodium Butyrate in Small Intestinal (IEC6) and Colonic (T84) Epithelial Cell Lines (1999)
    Springer
    Digestive diseases and sciences 44 (1999), S. 1571-1578 
    Details
    ISSN: 1573-2568
    Schlagwort(e): MINERALOCORTICOID RECEPTOR ; BUTYRIC ACID ; SMALL INTESTINAL CELLS ; COLON CELLS ; DIFFERENTIATION
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Mineralocorticoid action is essential forcolonic sodium and water absorption and is mediated viamineralocorticoid receptors in the upper half of coloniccrypts. On the other hand, it has been established that sodium butyrate inducesdifferentiation-like phenomenon in vitro. The aim ofthis study is to investigate whether this bacterialproduct participates in the regulation of gene andprotein expression of mineralocorticoid receptor in vitro. IEC6and T84 cells were stimulated by sodium butyrate andRNAs extracted. Gene expression of mineralocorticoidreceptor was evaluated by northern blotting orsemiquantitative RT-PCR. Protein expression was determined inT84 cells using immunohistochemistry. To investigatewhether MR induction was associated with cellulardifferentiation, we also measured alkaline phosphatase in situ. The mineralocorticoid receptor genewas induced by sodium butyrate in both IEC6 and T84cells. Immunoreactivity increased in butyrate-treatedT84 cells, but receptorcontaining cells were notuniformly distributed and often formed clusters.Induction of alkaline phosphate activity was alsodemonstrated in both IEC6 and T84 cells. Double stainingby immunoreactivity and alkaline phosphatase activityclearly demonstrated the colocalization of both afterbutyrate treatment. In conclusion, sodium butyrateup-regulates gene and protein expression of thefunctionally important mineralocorticoid receptor inepithelial cells, after induction by differentiation-likecondition in vitro.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1026606809171
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 6
    Digitale Medien
    Digitale Medien
    Type II 11β-Hydroxysteroid Dehydrogenase Expression in Human Colonic Epithelial Cells of Inflammatory Bowel Disease (1999)
    Springer
    Digestive diseases and sciences 44 (1999), S. 2516-2522 
    Details
    ISSN: 1573-2568
    Schlagwort(e): TYPE II 11β-HYDROXYSTEROIDDEHYDROGENASE ; ULCERATIVE COLITIS ; CROHN'S DISEASE
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Type II 11β-hydroxysteroid dehydrogenaseendows specificity on the mineralocorticoid receptor bymetabolizing cortisol and regulates sodium absorption inrenal and colonic epithelium. Altered expression of this enzyme may be associated with impairedsodium absorption often seen in colonic mucosa ofinflammatory bowel disease. The aim of this study was toinvestigate possible abnormality of11β-hydroxysteroid dehydrogenase protein and mRNA expression ininflammatory bowel disease. In Crohn's disease, thecolonic epithelium showed comparable levels ofimmunoreactivity and mRNA expression to those ofcontrol, except for the decreased immunoreactivity insevere inflamed lesions with deep ulcer. In contrary, alack or decrease of immunoreactivity was relevant inulcerative colitis regardless of the histological degree of inflammation. The mRNA expression wasalso significantly decreased in ulcerative colitis. Thisstudy demonstrates that abnormality of epithelial cellsin ulcerative colitis includes the enzyme that regulates water and sodium absorption,which are physiologically essential.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1026699324927
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
Schließen ⊗
Diese Webseite nutzt Cookies und das Analyse-Tool Matomo. Weitere Informationen finden Sie hier...