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  • 1
    Electronic Resource
    Electronic Resource
    DNA replication of parvovirus B 19 in a human erythroid leukemia cell line (JK-1) in vitro (1993)
    Springer
    Archives of virology 131 (1993), S. 201-208 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A major limitation of studies on the parvovirus B 19, a causative agent of transient aplastic crisis, has been the absence of appropriate cell lines permissive for the virus. In the present study, a human erythroid leukemia cell line (JK-1) was shown to support B 19 virus DNA replication in vitro. Forty-eight hours after virus inoculation of JK-1 liquid cell cultures, the average number of B 19 genome copies was estimated at 3,000 per cell by DNA dot blot analysis. The addition of erythropoietin increased B 19 copy number to 10,000 per cell. The presence of replicative forms of the B 19 virus genome was demonstrated by Southern blot analysis. Although persistent infection of B 19 virus was not observed in JK-1 cells, this culture system will be of value in elucidating the molecular basis of the erythroid specificity of parvovirus B 19.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01379092
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  • 2
    Electronic Resource
    Electronic Resource
    Cyclosporine A-associated fatal central nervous system angiopathy in a bone marrow transplant recipient: an autopsy case (2000)
    Springer
    Acta neuropathologica 99 (2000), S. 680-684 
    Details
    ISSN: 1432-0533
    Keywords: Key words Basilar artery ; Vasoconstriction ; Cyclosporine A ; Bone marrow transplantation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report here the case of a 32-year-old woman who suffered from a unique angiopathy in the central nervous system (CNS). She died of multiple infarcts in the brain stem and cerebellum during treatment with cyclosporine A after bone marrow transplantation for refractory anemia with excess of blasts. The autopsy findings showed segmental narrowing of the basilar artery, in which circumferential dissection of the internal elastic lamina had occurred. The distal portion of the basilar artery was obstructed by upward dislocation of the dissected intima. Similar angiopathy was also observed at multiple sites along the basilar artery branches. These findings suggest endothelial damage, including vasoconstriction and dissection of the CNS arteries.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010051179
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  • 3
    Electronic Resource
    Electronic Resource
    Cardiostimulatory action of coenzyme Q homologues on cultured myocardial cells and their biochemical mechanisms (1993)
    Springer
    Journal of molecular medicine 71 (1993), S. S71 
    Details
    ISSN: 1432-1440
    Keywords: Ubiquinone ; Cell culture ; Myocardium ; ATP ; Isoproterenol ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of coenzyme Q (CoQ) homologues on the beating of myocardial cells was investigated in cultured cell sheets from mouse fetuses and quail embryos. Myocardial cell sheets grown in Eagle's minimum essential medium with fetal bovine serum showed very weak and irregular beating when this serum was removed from the medium. However, the depressed beating rate and amplitude recovered almost completely within a few minutes by adding CoQ10 to the medium, and the effect of Co10 continued over 1 h. CoQ9 showed a cardiostimulatory effect similar to that of CoQ10 but CoQ8 and COQ7 showed almost no effect. Short homologues (less than CoQ4) inhibited the beating of cell sheets. The cardiostimulatory effect of CoQ10 was not blocked by atenolol, a selective β-blocker. In addition, CoQ10 stimulated the formation of ATP, not cAMP. CoQ0 and COQ3 inhibited beating rates by inhibiting ATP formation. In conclusion, only native CoQ homologues having a nona- or decaprenyl group showed a cardiostimulatory effect on cultured myocardial cells, probably by stimulating mitochondrial ATP formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226844
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  • 4
    Electronic Resource
    Electronic Resource
    Cloning and expression of the chicken immunoglobulin joining (J)-chain cDNA (2000)
    Springer
    Immunogenetics 51 (2000), S. 85-91 
    Details
    ISSN: 1432-1211
    Keywords: Key words J chain ; Polymeric immunoglobulin ; Ontogeny ; Evolution ; Comparative immunology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The J chain is a component of polymeric immunoglobulin (Ig) molecules and may play an important role in their polymerization and the transport of polymeric Ig across epithelial cells. In this study, the primary structure of the chicken J chain was determined by sequencing cDNA clones. The cDNA had an open reading frame of 476 nucleotides encoding a putative protein of 158 amino acid residues including the signal sequence. The 3′ untranslated region consisted of 1216 nucleotides and a poly(A) tail. The deduced amino acid sequence of the chicken J chain had a high degree of homology to that of human, cow, rabbit, mouse, frog, and earthworm, with eight conserved Cys residues identical to the mammalian J chains. Northern blot hybridization performed with total RNA from various chicken tissues revealed high levels of J-chain mRNA expression in spleen, intestine, Harderian gland, and bursa of Fabricius, and low levels in the thymus. The J chain was expressed in the bursa as early as day 15 of embryogenesis. These data indicated that the chicken J-chain gene displays a high degree of homology with that of other species, and is expressed at an early stage of development of the chicken immune system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050016
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  • 5
    Electronic Resource
    Electronic Resource
    Role of nuclear factor-κ B in the expression by tumor necrosis factor-α of the human polymeric immunoglobulin receptor (pIgR ) gene (2000)
    Springer
    Immunogenetics 51 (2000), S. 289-295 
    Details
    ISSN: 1432-1211
    Keywords: Key words Human ; Mucosa ; Gene regulation ; Cytokines ; Transcription factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  We analyzed the mechanism of human polymeric immunoglobulin receptor (pIgR) gene upregulation by tumor necrosis factor (TNF)-α. Northern blot analysis showed that the expression of pIgR mRNA was enhanced by TNF-α stimulation. This activation was completely inhibited by RNA polymerase or protein synthesis inhibitors, suggesting that the regulation of pIgR gene expression depends on de novo RNA and protein synthesis. Furthermore, the stimulation of pIgR mRNA by TNF-α was decreased by pyrrolidinedithiocarbamate and l-1-4′-tosylamino-phenylethyl-chloromethyl ketone, which are known nuclear factor (NF)-κB inhibitors. For further analysis of gene regulation, we cloned and sequenced the 1.5-kb 5′-flanking region of the pIgR gene. In the upstream region, we found two NF-κB-binding motifs (named κB1 and κB2 from the 5′ region). An electrophoretic mobility shift assay indicated that two components of the NF-κB/Rel family, p50 and p65, bound with higher affinity to the κB2 element than to the κB1 element. We also analyzed pIgR gene expression using reporter plasmids expressing the firefly luciferase gene. Stimulation by TNF-α significantly activated the pIgR gene promoter, as a 775-bp upstream region of the pIgR gene increased luciferase gene expression in cells treated with TNF-α. The activation of promoter activity by TNF-α was abolished when a mutation was inserted into κB1 or κB2. These data indicated that pIgR gene expression induced by TNF-α is transcriptionally regulated via activation of NF-κB. In addition, there is a possibility that another factor may act in concert with NF-κB.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050622
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  • 6
    Electronic Resource
    Electronic Resource
    Simulation study on dynamics transition in neuronal activity during sleep cycle by using asynchronous and symmetry neural network model (1990)
    Springer
    Biological cybernetics 63 (1990), S. 243-250 
    Details
    ISSN: 1432-0770
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Computer Science , Physics
    Notes: Abstract We have found that single neuronal activities in different regions in the brain commonly exhibit the distinct dynamics transition during sleep-waking cycle in cats. Especially, power spectral densities of single neuronal activities change their profiles from the white to the 1/f along with sleep cycle from slow wave sleep (SWS) to paradoxical sleep (PS). Each region has different neural network structure and physiological function. This suggests a globally working mechanism may be underlying the dynamics transition we concern. Pharmacological studies have shown that a change in a wide-spread serotonergic input to these regions possibly causes the neuronal dynamics transition during sleep cycle. In this paper, based on these experimental results, an asynchronous and symmetry neural network model including inhibitory input, which represents the role of the serotonergic system, is utilized to examine the reality of our idea that the inhibitory input level varying during sleep cycle induce that transition. Simulation results show that the globally applied inhibitory input can control the dynamics of single neuronal state evolution in the artificial neural network: 1/f-like power spectral density profiles result under weak inhibition, which possibly corresponds to PS, and white profiles under strong inhibition, which possibly corresponds to SWS. An asynchronous neural network is known to change its state according to its energy function. The geometrical structure of network energy function is thought to vary along with the change in inhibitory level, which is expected to cause the dynamics transition of neuronal state evolution in the network model. These simulation results support the possibility that the serotonergic system is essential for the dynamics transition of single neuronal activities during sleep cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00203447
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  • 7
    Electronic Resource
    Electronic Resource
    Mapping the fermi surface of graphite with a display-type photoelectron spectrometer (1991)
    Springer
    Applied physics 52 (1991), S. 299-301 
    Details
    ISSN: 1432-0630
    Keywords: 79.60.Cn ; 71.25.Pi ; 71.25.Hc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A display spectrometer is used to image the momentum distribution of photoelectrons from the Fermi level in graphite. The Fermi “surface” consists of six points at the corners K of the hexagonal, two-dimensional Brillouin zone, in agreement with band theory. The method is also applied to other equal energy surfaces below the Fermi level, thereby giving the band dispersion of the π-bands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00324768
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  • 8
    Electronic Resource
    Electronic Resource
    Characterization of Bulk Cementite Produced by Mechanical Alloying and Spark Plasma Sintering (2003)
    s.l. ; Stafa-Zurich, Switzerland
    Journal of metastable and nanocrystalline materials Vol. 15-16 (Apr. 2003), p. 607-614 
    Details
    ISSN: 1422-6375
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/43/transtech_doi~10.4028%252Fwww.scientific.net%252FJMNM.15-16.607.pdf
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  • 9
    Electronic Resource
    Electronic Resource
    Remote epileptogenic focus detected by electrocorticogram in a case of cavernous angioma (1994)
    Springer
    Acta neurochirurgica 127 (1994), S. 236-239 
    Details
    ISSN: 0942-0940
    Keywords: Cavernous angioma ; ECoG ; epileptogenic focus ; surgical treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In a case of cavernous angioma, a remote gyrus, which was found to contain an epileptogenic focus by intraoperative electrocorticography (ECoG), was resected simultaneously with lesionectomy. The patient was a 27-year-old male who was referred to our hospital because of frequent systemic tonic-clonic convulsions. ECoG revealed an epileptogenic focus not only in the cortex around the angioma-affected tissue of the left frontal lobe but also in an in addition to lesionectomy. The postoperative course was uneventful. Now (two years after surgery), the patient is seizure-free In the surgical treatment of convulsions-accompanied by cavernous angioma, it is essential not only to detect epileptogenic foci by intraoperative ECoG but also to remove these foci together with the angioma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01808773
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  • 10
    Electronic Resource
    Electronic Resource
    Management of giant serpentine aneurysms of the middle cerebral artery — Review of literature and report of a case successfully treated by STA-MCA anastomosis only (1992)
    Springer
    Acta neurochirurgica 117 (1992), S. 23-29 
    Details
    ISSN: 0942-0940
    Keywords: Giant aneurysm ; serpentine aneurysm ; thrombosed aneurysm ; STA-MCA anastomosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Thirty-eight cases of giant serpentine aneurysms (GSA), including 17 GSA of the middle cerebral artery (MCA), were reviewed in the literature. The treatment possibilities of GSA of the MCA are discussed together with our own case who was a 39-year-old male with a GSA of the right MCA and was treated only by STA-MCA anastomosis. The pathogenetic mechanism of progressive enlargement of the aneurysm is also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01400630
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