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  • 1
    Digitale Medien
    Digitale Medien
    Polymerase chain reaction-single strand conformation polymorphism analysis of the p53 gene in paraffin-embedded surgical material from human renal cell carcinomas (1994)
    Springer
    Virchows Archiv 424 (1994), S. 229-233 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Renal cell carcinoma ; p53 tumour suppressor gene ; Polymerase chain reaction ; Single strand conformation polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract p53 tumour suppressor gene mutations were studied in 118 renal cell carcinomas using paraffin-embedded surgical material. Optimal results were obtained with analysis of exon lengths between 150 and 200 base pairs for polymerase chain reaction. Single strand conformation polymorphism and sequencing analysis revealed only two point mutations (2/118, 2%): one involving codon 135; TGC→TTC (cysteine→phenylalanine) and the other codon 175; CGC→CAC (arginine→histidine). Both of these cases were classified as granular cell subtype on microscopic observation. The data suggest that the p53 tumour suppressor gene is not related to tumour initiation, promotion, or progression of renal cell carcinomas. However, there is the possibility that granular cell type carcinomas may have a different genetic background from clear cell type renal neoplasms.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00194605
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Selective planting of cationized, haptentized ovalbumin on the rat tubular basement membrane (1994)
    Springer
    Virchows Archiv 424 (1994), S. 587-591 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Tubular basement membrane ; Peritubular capillary ; Cationic antigen ; Ovalbumin ; Trinitrophenol
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We developed an experimental protocol for planting exogenous antigens with different molecular weights and charges on the constituents of the renal tubulointerstitium. The cationized antigens were injected selectively into the left renal arteries of Wistar rats. Antigen localization was documented by immunohistochemistry on frozen sections. Cationized bovine serum albumin (BSA; 68 kDa, isoelectric point =9.5) localized almost exclusively along the glomerular capillary wall. After application of highly cationic polyethyleneimine, cationized BSA given subsequently was found in a linear distribution along the glomerular capillary wall and along the peritubular capillaries. The fate of highly cationized ovalbumin conjugated with trinitrophenol (TNP-OA), subjected to gel filtration to obtain monomers (42 kDa, isoelectric point 〉10) differed; it was deposited in a linear pattern on the tubular basement membrane (TBM) and Bowman's capsule, and remained up to 36 h after injection. Noncationized, monomeric TNP-OA (42 kDa, isolectnic point =4.6) showed fine granular deposition in the tubular epithelium exclusively. These findings indicate that the barrier of the glomerular BM acts selectively on antigens with different molecular weights. They either settle on the peritubular capillaries, after passing the glomerular, or reach the urinary space, after which they are reabsorbed by the tubular epithelial cells to reach the TBM.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00195771
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Selective planting of cationized, haptenized ovalbumin on the rat tubular basement membrane (1994)
    Springer
    Virchows Archiv 424 (1994), S. 587-591 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Tubular basement membrane ; Peritubular capillary ; Cationic antigen ; Ovalbumin ; Trinitrophenol
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We developed an experimental protocol for planting exogenous antigens with different molecular weights and charges on the constituents of the renal tubulointerstitium. The cationized antigens were injected selectively into the left renal arteries of Wistar rats. Antigen localization was documented by immunohistochemistry on frozen sections. Cationized bovine serum albumin (BSA; 68 kDa, isoelectric point =9.5) localized almost exclusively along the glomerular capillary wall. After application of highly cationic polyethyleneimine, cationized BSA given subsequently was found in a linear distribution along the glomerular capillary wall and along the peritubular capillaries. The fate of highly cationized ovalbumin conjugated with trinitrophenol (TNP-OA), subjected to gel filtration to obtain monomers (42 kDa, isoelectric point 〉10) differed; it was deposited in a linear pattern on the tubular basement membrane (TBM) and Bowman's capsule, and remained up to 36 h after injection. Noncationized, monomeric TNP-OA (42 kDa, isolectnic point =4.6) showed fine granular deposition in the tubular epithelium exclusively. These findings indicate that the barrier of the glomerular BM acts selectively on antigens with different molecular weights. They either settle on the peritubular capillaries, after passing the glomerular, or reach the urinary space, after which they are reabsorbed by the tubular epithelial cells to reach the TBM.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01069737
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Superoxide anion production and expression of cytochrome b 558 by neutrophils are impaired in some patients with myelodysplastic syndrome (1991)
    Springer
    Annals of hematology 63 (1991), S. 270-275 
    Details
    ISSN: 1432-0584
    Schlagwort(e): Neutrophils ; Myelodysplastic syndromes ; Superoxide ; Prednisolone ; Cytochrome b
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Superoxide anion (O2 −) production and expression of cytochrome b558 by neutrophils were determined in 20 patients with myelodysplastic syndrome (MDS). The reduction of O2 − production was noted in eight of the 20 patients and an increase was noted in four patients when neutrophils were stimulated byn-formylmethionyl-leucyl-phenylalanine (FMLP), while a low level of O2 − production was found in 11 and an increase in six MDS patients when they were stimulated by phorbol myristate acetate (PMA). Among them, seven patients showed a decrease and four an increase in O2 − production on stimulation with either FMLP or PMA. Expression of cytochrome b 558 was found to be at low levels in patients who had neutrophils showing decreased O2 − production when stimulated with FMLP, indicating that decreased expression of cytochrome b558 might contribute to the impairment of O2 − production in some MDS patients. In this study, no significant differences in O2 − production were noted among subtypes of MDS; however, the patients who had received prednisolone showed lower levels of O2 − production than those who had not received prednisolone. Patients manifesting episodes of infection had reduced levels of O2 − production compared with those without infection. Furthermore, the fact that one patient who exhibited a marked reduction in neutrophil counts together with reduced O2 − production died of fatal infection, suggests that the determination of O2 − production, in combination with hematological features, may be of some help in predicting severe infection.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01698377
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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