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  • 1
    Electronic Resource
    Electronic Resource
    Fermentation of crystalline cellulose to ethanol by Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes (1993)
    s.l. : American Chemical Society
    Biotechnology progress 9 (1993), S. 533-538 
    Details
    ISSN: 1520-6033
    Source: ACS Legacy Archives
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bp00023a013
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  • 2
    Electronic Resource
    Electronic Resource
    Ethanol production from starch by recombinantEscherichia coli containing integrated genes for ethanol production and plasmid genes for saccharification (1992)
    Springer
    Biotechnology letters 14 (1992), S. 415-420 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Ethanologenic strains ofEscherichia coli have been developed which can express thermostable enzymes for starch saccharification as intracellular products. These enzymes can be harvested within cells at the end of fermentation and liberated by heating to the temperature at which they exhibit maximal activity (60°C to 70°C). Organisms such as these could be used to supply enzymes for yeast-based fermentations while producing ethanol as a co-product.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01021257
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  • 3
    Electronic Resource
    Electronic Resource
    Conversion of hydrolysates of corn cobs and hulls into ethanol by recombinantEscherichia coli B containing integrated genes for ethanol production (1992)
    Springer
    Biotechnology letters 14 (1992), S. 857-862 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Hemicellulose and residual starch in corn hulls from wet milling and hemicellulose in corn cobs were hydrolyzed by incubation in dilute sulfuric acid at 140°C to 160°C. These hydrolysates were efficiently fermented to ethanol by a genetically engineered derivative ofE. coli B, strain KO11. Fermentation of com hull hydrolysate was complete after 48 h with a final ethanol concentration of 38 grams per liter. Fermentation of corn cob hydrolysate was essentially complete after 24 h due to a lower concentration of sugars and higher levels of inocula. In both cases, ethanol produced was equivalent to 100% of the maximum theoretical yield (0.51 grams ethanol/gram sugar) based on momoner sugar content. ThusE. coli B strain KO11 appears to be an excellent candidate for the efficient production of ethanol from hydrolysates of corn residues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01029153
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  • 4
    Electronic Resource
    Electronic Resource
    Fermentation of galacturonic acid and other sugars in orange peel hydrolysates by the ethanologenic strain of Escherichia coli (1994)
    Springer
    Biotechnology letters 16 (1994), S. 281-286 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Enzymatic hydrolysates of orange peel contain relatively high levels of galacturonic acid and arabinose which are not fermentable to ethanol by yeasts. We observed complete utilization of both sugars during fermentation of peel hydrolysates by the ethanologenic construct of E. coli KO11. The bacterium exhibits a novel pattern of galacturonic acid fermentation producing equimolar amounts of acetate and ethanol accompanied by carbon dioxide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00134626
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  • 5
    Electronic Resource
    Electronic Resource
    Genetic engineering of soft-rot bacteria for ethanol production from lignocellulose (1993)
    Springer
    Journal of industrial microbiology and biotechnology 11 (1993), S. 151-155 
    Details
    ISSN: 1476-5535
    Keywords: Erwinia ; Lignocellulose ; Cellulose ; Ethanol ; Cellulase ; Xylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The soft-rot bacteriaErwinia carotovora SR38 andErwinia chrysanthemi EC16 have been genetically engineered to efficiently produce ethanol and carbon dioxide as primary fermentation products from cellobiose, glucose and xylose. These organisms have the native ability to secrete a battery of hydrolases and lyases to aid in the solubilization of lignocellulose. Both strains of ethanologenicErwinia fermented cellobiose at twice the rate of the cellobioseutilizing yeasts (Spindler et al., 1992. Biotechnology Letters 14: 403–407) and may be useful in simultaneous saccharification and fermentation processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01583716
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  • 6
    Electronic Resource
    Electronic Resource
    Fermentation of sweet whey by ethanologenic Escherichia coliFlorida Agricultural Experiment Station Publication Number R-01831. (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 40 (1992), S. 41-45 
    Details
    ISSN: 0006-3592
    Keywords: lactose ; whey ; E. coil ; ethanol ; kluyveromyces fragilis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Whey, an abundant byproduct of the dairy industry, contains large amounts of protein and lactose which could be used for fuel ethanol production. We have investigated a new organism as a candidate for such fermentations: recombinant Escherichia coli containing the genes encoding the ethanol pathway from Zymomonas mobilis. The highest level of ethanol achieved, 68 g/L, was produced after 108 hours in Luria broth containing 140 g lactose/L. Fermentations of lower lactose concentrations were completed more rapidly with approximately 88% of theoretical yields. Reconstituted sweet whey (60 g lactose/L)was fermented more slowly than lactose in Luria broth requiring 144 hours to produce 26 g ethanol/L. Supplementing sweet whey with a trace metal mix and ammonium sulfate reduced the required fermentation time to 72 hours and increased final ethanol concentration (28 g ethanol/L). By adding proteinases during fermentation, the requirement for ammonia was completely eliminated, and the rate of fermentation further improved (30 g ethanol/L after 48 hours). This latter incresed in rate of ethanol production and ethanol yield are presumed to result from incorporation of amino acids released by hydrolysis of whey proteins. The fermentation of sweet whey by ethanologenic E. coil reduced the nonvolatile residue by approximately 70%. This should reduce biological oxygen demand and reduce the cost of waste treatment. Whey supplemented with trace metals and small amounts of proteinase may represent an economically attractive feedstock for the production of ethanol and other useful chemicals.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260400107
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  • 7
    Electronic Resource
    Electronic Resource
    Parametric studies of ethanol production form xylose and other sugars by recombinant Escherichia coliFlorida Agricultural Experiment Station Publication Number R-01082. (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 38 (1991), S. 296-303 
    Details
    ISSN: 0006-3592
    Keywords: ethanol ; genetic engineering ; Escherichia coli ; lignocellulose ; xylose ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The conversion of xylose to ethanol by recombinant Escherichia coli has been investigated in pH-controlled batch fermentations. Chemical and environmental parameters were varied to determine tolerance and to define optimal conditions. Relatively high concentrations of ethanol (56 g/L) were produced from xylose with excellent efficiencies. Volumetric productivities of up to 1.4 g ethanol/L h were obtained. Productivities, yields, and final ethanol concentrations achieved from xylose with recombinant E. coli exceeded the reported values with other organisms. In addition to xylose, all other sugar constituents of biomass (glucose, mannose, arabinose, and galactose) were efficiently converted to ethanol by recombinant E. coli. Unusually low inocula equivalent to 0.033 mg of dry cell weight/L were adequate for batch fermentations. The addition of small amounts of calcium, magnesium, and ferrous ions stimulated fermentation. The inhibitory effects of toxic compounds (salts, furfural, and acetate) which are present in hemicellulose hydrolysates were also examined.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260380311
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  • 8
    Electronic Resource
    Electronic Resource
    Saccharification and fermentation of Sugar Cane bagasse by Klebsiella oxytoca P2 containing chromosomally integrated genes encoding the Zymomonas mobilis ethanol pathway (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 44 (1994), S. 240-247 
    Details
    ISSN: 0006-3592
    Keywords: lignocellulose ; ethanol ; Klebisella oxytoca ; fermentation ; cellulase ; cellulose ; cellobiose ; biomass ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Pretreatment of sugar cane bagasse is essential for a simultaneous saccharification and fermentation (SSF) process which uses recombinant Klebsiella oxytoca strain P2 and Genencor Spezyme CE. Strain P2 has been genetically engineered to express Zymomonas mobilis genes encoding the ethanol pathway and retains the native ability to transport and metabolize cellobiose (minimizing the need for extracellular cellobiase). In SSF studies with this organism, both the rate of ethanol production and ethanol yield were limited by saccharification at 10 and 20 filter papaer units (FPU) g-1 acid-treated bagasse. Dilute slurries of biomass were converted to ethanol more efficiently (over 72% of theoretical yield) in simple batch fermentations than slurries containing high solids albeit with the production of lower levels of ethanol. With high solids (i.e., 160 g acid-treated bagasse L-1), a combination of 20 FPU cellulase g-1 bagasse, preincubation under saccharification conditions, and additional grinding (to reduce particle size) were required to produce ca. 40 g ethanol L-1. Alternatively, almost 40 g ethanol L-1 was produced with 10 FPU cellulase g-1 bagasse by incorporating a second saccharification step (no further enzyme addition) followed by a second inoculation and short fermentation. In this way, a theoretical ethanol yield of over 70% was achieved with the production of 20 g ethanol 800 FPU-1 of commercial cellulase. © 1994 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260440213
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  • 9
    Electronic Resource
    Electronic Resource
    Expression of theZymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes inBacillus (1994)
    Springer
    Current microbiology 28 (1994), S. 279-282 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The genes encodingZymomonas mobilis pyruvate decarboxylase (pdc) and alcohol dehydrogenase II (adhB) were expressed inBacillus subtilis YB886(pLOI500) under the control of aBacillus SPO2 phage promoter and caused a 50% reduction of growth rate compared with the unmodified vector. Expression was further confirmed by Western blots, activity stains of native gels, and in vitro measurements of alcohol dehydrogenase activity. Additional strains ofBacillus were also transformed, and all produced similar but low levels of these enzymes. Although higher specific activities will be required for efficient ethanol production, no fundamental barriers exist to the expression of theseZ. mobilis genes inBacillus. Two abundant new proteins (ca. mass 33,000 daltons and 14,000 daltons) were observed in Coomassie Blue-stained gels; they are similar in size to the proteins induced by recombinatn products inEscherichia coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01573206
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