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  • 1
    Electronic Resource
    Electronic Resource
    Phylogenetic analysis of the stress-70 protein family (1994)
    Springer
    Journal of molecular evolution 39 (1994), S. 80-86 
    Details
    ISSN: 1432-1432
    Keywords: Heat-shock proteins ; HSP70 ; Stress-70 ; Phylogenetic trees ; Protein families ; NLS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The eukaryotic cyto-/nucleoplasmatic 70-kDa heat-shock protein (HSP70) has homologues in the endoplasmic reticulum as well as in bacteria, mitochondria, and plastids. We selected a representative subset from the large number of sequenced stress-70 family members which covers all known branches of the protein family and calculated and manually improved an alignment. Here we present the consensus sequence of the aligned proteins and putative nuclear localization signals (NLS) in the eukaryotic HSP70 homologues. The phylogenetic relationships of the stress-70 group family members were estimated by use of different computation methods. We present a phylogenetic tree containing all known stress-70 subfamilies and demonstrate the usefulness of stress-70 protein sequences for the estimation of intertaxonic phylogeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00178252
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  • 2
    Electronic Resource
    Electronic Resource
    Phylogenetic relationships of HMG box DNA-binding domains (1993)
    Springer
    Journal of molecular evolution 37 (1993), S. 204-210 
    Details
    ISSN: 1432-1432
    Keywords: HMG box ; DNA-binding proteins ; High-mobility-group (HMG) proteins ; Phylogenetic trees ; Nuclear targeting sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract HMG boxes were initially identified as DNA-binding domains of the human RNA polymerase I (pol I) transcription factor hUBF and the animal high-mobility-group (HMG) protein family HMG1. Since then, numerous sequences of HMG-box-containing HMG proteins and other DNA-binding proteins from several species have become available. By sequence comparisons of a selected range of HMG boxes from these proteins and the construction of phylogenetic trees we show that the HMG box is highly conserved between DNA-binding proteins of organisms from all three eukaryotic kingdoms and that HMG boxes are linked by distinct evolutionary relationships. In addition, most HMG boxes display comparable hydropathy profiles and amino acid arrangements, which could serve as nuclear targeting sequences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02407357
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  • 3
    Electronic Resource
    Electronic Resource
    Evolutionary analysis of the plastid-encoded gene for the α subunit of the DNA-dependent RNA polymerase of Pyrenomonas salina (cryptophyceae) (1992)
    Springer
    Current genetics 22 (1992), S. 479-482 
    Details
    ISSN: 1432-0983
    Keywords: Pyrenomonas salina (Cryptophyceae) ; Plastid ; DNA-dependent RNA polymerase α subunit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nucleotide sequence of the gene coding for the plastid-encoded α subunit of DNA-dependent RNA polymerase from the cryptomonad alga Pyrenomonas salina was determined. The deduced amino-acid sequence, corresponding to a 35.2 kDa polypeptide, was compared to homologues from other organisms. Evolutionary relationships were analyzed in detail by the parsimony method together with bootstrap analysis. The deduced phylogenetic tree shows that the cryptomonad gene is the most ancient type of known plastid-encoded RNA polymerase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00326413
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  • 4
    Electronic Resource
    Electronic Resource
    Atomic-scale view of AlGaAs/GaAs heterostructures with cross-sectional scanning tunneling microscopy (1993)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 63 (1993), S. 1273-1275 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Atomically resolved cross-sectional scanning tunneling microscope topographic images of heterostructures that include a sequence of 1, 2, 5, and 10 nm AlGaAs and GaAs layers are presented. These layers clearly show alloy fluctuations and interface roughness on an atomic scale. In the thick AlGaAs layers a mottled structure with regions of higher Al content about 2 nm wide and elongated in [1¯12] or [11¯2] directions are observed. Similarly, the interfaces are rough on a 2 nm length scale. These results suggest that, for the conditions used for the epitaxial growth of the ternary layers, Al-rich regions nucleate and grow anisotropically.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.109755
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  • 5
    Electronic Resource
    Electronic Resource
    The presence of a nucleomorph hsp70 gene is a common feature of Cryptophyta and Chlorarachniophyta (1994)
    Springer
    Current genetics 26 (1994), S. 451-455 
    Details
    ISSN: 1432-0983
    Keywords: Chlorarachniophyta ; Cryptophyta ; Nucleomorph ; HSP 70
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cryptomonad algae and Chlorarachiniophyta are evolutionary chimaeras derived from the engulfment of an eukaryotic phototrophic endosymbiont by a eukaryotic host cell. Although much reduced, the endosymbiont's eukaryotic plasmatic compartment still contains a nucleus, the so-called nucleomorph. These nucleomorphs carry the smallest known eukaryotic genomes. We have characterized the genomes of several cryptomonads and a Chlorarachnion species by means of PFGE (pulsed-field gel electrophoresis). Hybridization studies with small subunit rDNA were used to identify the nucleomorph chromosomes. We also performed hybridization experiments with an hsp70 probe to estimate the distribution of this gene among the different algal species. The evolutionary, genetical, and physiological implications of our studies are discussed. A model on the possible function of the nucleomorph hsp70 gene products is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00309933
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  • 6
    Electronic Resource
    Electronic Resource
    Andrological findings in young patients under long-term antidepressive therapy with clomipramine (1994)
    Springer
    Psychopharmacology 116 (1994), S. 357-359 
    Details
    ISSN: 1432-2072
    Keywords: Clomipramine ; Erectile dysfunction ; Spermiogram ; Sexual endocrine profiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Serum hormone levels of follicle stimulating hormone (FSH), luteotropic hormone (LH), testosterone (T), prolactin (hPRL), estradiol (E2) and GnRH test were carried out in 11 patients after antidepressive therapy with clomipramine at a dosage of 75 mg/day for 3 months. Nine of these patients agreed with the evaluation of ejaculate parameter. As an age matched control group 15 patients without psychiatric disorders but under urological treatment because of erectile dysfunction were investigated in the same manner, with 11 patients being able to produce an ejaculate for examination. Both groups were closely comparable caccording to age, percentage of proven fertility and urological status. All spermiograms evaluated in the clomipramine group were pathological, especially with regard to volume, sperm motility and sperm morphology, whereas only 37% in the control group showed pathological findings, approximately the same as that of healthy individuals in this age range. Serum hormone levels as well as the hypothalamic-hypophyseal-gonadal axis proven with the Gn-RH test were in normal range in both groups. According to these results it seems evident that clomipramine does not alter sexual hormone profiles in males or interfere with the hypothalamic-hypophyseal-gonadal axis and has a significant negative effect on ejaculate parameters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02245340
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  • 7
    Electronic Resource
    Electronic Resource
    The smallest known eukaryotic genomes encode a protein gene: towards an understanding of nucleomorph functions (1994)
    Springer
    Molecular genetics and genomics 243 (1994), S. 600-604 
    Details
    ISSN: 1617-4623
    Keywords: Cryptomonads ; Periplastidal ; Hsp70 ; Transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cryptomonads are unicellular algae with plastids surrounded by four membranes. Between the two pairs of membranes lies a periplastidal compartment that harbours a DNA-containing organelle, termed the nucleomorph. The nucleomorph is the vestigial nucleus of a phototrophic, eukaryotic endosymbiont. Subcloning of parts of one nucleomorph chromosome revealed a gene coding for an Hsp70 protein. We demonstrate the expression of this nucleomorph protein-coding gene and present a model for protein transport from the host to the endosymbiont compartment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00284209
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  • 8
    Electronic Resource
    Electronic Resource
    Molecular Cloning. A Laboratory Manual. 2. Auflage. Hrsg. von J. Sambrook, E. F. Fritsch, T. Maniatis, Cold Spring Harbor Laboratory Press, Cold Spring Harbour 1989, $ 115. ISBN 0-87969-309-6 (1990)
    Weinheim : Wiley-Blackwell
    Biologie in unserer Zeit 20 (1990), S. 285-285 
    Details
    ISSN: 0045-205X
    Keywords: Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/biuz.19900200607
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