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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 83 (1992), S. 467-475 
    ISSN: 1432-2242
    Keywords: Malus pumila Mill ; Malus species ; Esterase ; Genes EST-1, EST-3, EST-4
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three main zones of esterase activity (EST-I, EST-III, EST-IV) identified in leaf extracts of cultivated apple and Malus species were determined by the genes EST-1, EST-3 and EST-4, respectively. In addition to earlier reported alleles of EST-1 (a, b) three further bands c, d and f were identified in the EST-I zone of which c was found to be determined by an allele, c. Two alleles, a, b, and a null allele were found for both the genes EST-3 and EST-4. Differences in allelic frequency were observed between cultivars, rootstocks and Malus species. Allele EST-1a was rare amongst the rootstocks. The examination of Malus species and derivatives showed a geographical relationship. Allele EST-1c was confined to species of Asian origin, and EST-1d was confined to American species.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 83 (1992), S. 345-352 
    ISSN: 1432-2242
    Keywords: Malus pumila Mill ; Leucine aminopeptidase ; Genes LAP-1, LAP-2, LAP-3, LAP-4 ; Mildew resistance ; Linkage ; Breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Six zones of LAP activity were detected in apples, some of them tissue specific. Genetic studies in four of them revealed the presence of four genes LAP-1, LAP-2, LAP-3 and LAP-4 with 4, 5, 4 and 4 alleles respectively including two null alleles. There were no big differences in allelic frequency within cultivars, selections, rootstocks and Malus species. Close linkage was found between LAP-2 and resistance to mildew derived from ‘White Angel’.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 83 (1992), S. 392-399 
    ISSN: 1432-2242
    Keywords: Malus pumila Mill ; Peroxidase ; Genes PRX-1, PRX-2, PRX-3, PRX-4, PRX-5, PRX-6, PRX-7, PRX-8 ; Linkage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Six anodal and two cathodal zones of peroxidase activity were observed in apple and designated PRX-I to PRX-VIII from the most anodal to the most cathodal on the basis of migrational distance. PRX phenotypes were found to be coded by at least eight genes (PRX-1-8); analyses of four (PRX-2, PRX-3, PRX-4 and PRX-7) revealed 15 alleles including three null alleles. PRX-2 and PRX-3 were closely linked, and PRX-1 appeared to be in the same linkage group. In addition, PRX-4 and PRX-5 were linked.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5060
    Keywords: Malus ; apple ; genetic mapping ; isozymes ; DNA techniques ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A recently initiated collaborative project involving apple breeders in seven European countries is described. The objective is to improve the European apple crop by molecular-aided breeding to increase efficency and reduce the time-scale in breeding for resistance, tree habit and fruit quality. The strategy adopted provides a model for similar studies in fruit, forest and other woody species. The project is based on progenies from a small number of crosses involving many important agronomic genes. Replication of these reference progenies by vegetative propagation will enable studies to be carried out simultaneously in each country. By developing a range of molecular markers, including isozymes, RFLPs and sequence-tagged DNA probes, an integrated molecular map is being constructed for use in a wide range of breeding and genetic studies. Construction of a database recording many mapped molecular markers will enable efficient exploitation of data in future genetic, breeding and physiological studies of apple. Aspects of the adopted strategy, techniques and management are discussed in the context of mapping genes in perennial crop genomes.
    Type of Medium: Electronic Resource
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