ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
We examined (he role of monocytes in T-cell activation induced by phorbol myristate acetate (PMA) and calcium ionophore ionomycin. Depletion of monocytes from peripheral blood mononuclear cells (PBMC) was associated with the loss of inlerleukin-2 (IL-2) production, IL-2 receptor (1L-2 RI expression and proliferation, in response to cither PMA or ionomycin. Addition of monocytes to highly purified T cells resulted in the complete R-constitution of IL-2 production. 1L-2R expression and proliferation by PMA-stimulated lymphocytes. Exogenous IL-2, but not interleukin-l (IL-l), could reconstitute the T-cell responsiveness. Addition of monocytes to highly purified T cells stimulated with ionomycin resulted in partial reconstitution of IL-2 production. II,-2R expression and proliferation. Similarly, (he addition of exogenous lL-2 to ionomycin-stimulated T cells only partially reconstituted the response compared with PBMC, These results suggest that monocyte-T-cell interactions contribute to IL-2 production and IL-2R expression and are crucial events for PMA-induced T-cell proliferation. With ionomycin, monocytes play a role, in part, in inducing IL-2 production, IL-2R expression and proliferation. However, IL-2 is not a sufficient signal to induce T-cell proliferative response lo ionomycin, suggesting that an IL-2-independent mechanism may exist in ionomycin-induced T-cell proliferation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3083.1991.tb01787.x
Permalink
