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  • 1
    Electronic Resource
    Electronic Resource
    P44
A case of contact dermatitis caused by a NSAID’s soluble agent (2004)
    Oxford, UK; Malden, USA : Blackwell Science Ltd/Inc.
    Contact dermatitis 50 (2004), S. 0 
    Details
    ISSN: 1600-0536
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A fifty five year-old man had experienced itching sensation when he applied analgesic plasters for his lumbago. He tried to use several kinds of analgesic plasters, however he felt itching sensation whenever he used them. He applied a plaster containing ferbinac for his left elbow joint’s pain on April 2003. On the next day, an itching erythema developed on the area where the plaster was applied. He was treated with a difluprednate ointment, and his dermatitis gradually improved. He visited our clinic for precise medical examination for finding out the causative agent of his dermatitis on May 13, 2003. We conducted 48 hours closed patch testing with the plaster he used and it’s ingredients. He reacted positively to the plaster containing ferbinac and crotamiton 5%p that was used for dissolute the active drug. He also reacted positively to a cream containing ketoprofen, however he reacted negatively to ketoprofen 1%p. Crotamoton was also used in the ketoprofen cream.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0105-1873.2004.0309fz.x
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  • 2
    Electronic Resource
    Electronic Resource
    The Chloroplast Chromosomes in Land Plants (1989)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Cell and Developmental Biology 5 (1989), S. 51-70 
    Details
    ISSN: 0743-4634
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.cb.05.110189.000411
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  • 3
    Electronic Resource
    Electronic Resource
    Immunological cell situation in the skin of atopic model mice (2004)
    Oxford, UK : Blackwell Publishing Ltd.
    Journal of the European Academy of Dermatology and Venereology 18 (2004), S. 0 
    Details
    ISSN: 1468-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background  We observed nishikinezumi, cinnamon-coloured (NC)/Fujita (F) mice aged between 5 and 28 weeks. These NC mice have skin eruptions that resemble human atopic dermatitis (AD) under conventional circumstances.Object  We investigated the skin of eruptive and non-eruptive lesions in NC/F mice by using haematoxylin–eosin (H&E) staining, toluidine blue staining and immunohistopathological study with immunoglobulin (Ig)EɛRI, CD23, interleukin (IL)-4, IL-5, interferon (INF)-γ and Ia antigen.Results  Histological examination of the eruptive lesions revealed the perivascular infiltration of many lymphocytes and mast cells into the upper dermis. Intracellular oedema of the epidermis, lymphocyte infiltration into the epidermis and liquefaction degeneration of the basal layer were also observed. The numbers of IL-4 and IL-5 positive cells in the eruptive lesions were larger than those of the non-eruptive lesions. IL-4 and IL-5 positive cells in the eruptive lesions increased weekly. Some IFN-γ positive cells were observed in the eruptive lesions after 21 weeks. IFN-γ positive cells were scarce in the skin of both the non-eruptive and eruptive lesions before 21 weeks. Serum IgE increased from 7 weeks to 21 weeks.Discussion  We confirmed that these findings indicated that T helper (Th)2-dominant immunological activation transformed to a Th1-dominant situation. Many IgEɛRI positive cells were recognized in the dermis of the eruptive lesions by the time IgE had decreased. We assumed that the dermatitis before 21 weeks was an IgE-mediated allergy. We have previously reported that older NC/F mice had positive patch-test reactions to mites. Because serum IgE decreased after 21 weeks, dermatitis after 21 weeks might be associated more with cell-mediated delayed hypersensitivity than with IgE-mediated immediate allergy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1468-3083.2004.00855.x
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  • 4
    Electronic Resource
    Electronic Resource
    Location and nucleotide sequence of the genes for tobacco chloroplast tRNAArg (ACG) and tRNALeu(UAG) (1985)
    Springer
    Current genetics 9 (1985), S. 405-409 
    Details
    ISSN: 1432-0983
    Keywords: Chloroplast ; tRNA gene ; tRNA-like sequence ; Open reading frame
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The location and nucleotide sequence of the genes and flanking regions for tRNAArg(ACG) and tRNALeu(UAG) on tobacco chloroplast DNA have been determined. The gene arrangement is 5S rRNA - 260 bp -tRNAArg - 581 by - tRNAAsn - 5.2 kbp - tRNALeu. The tRNAArg and tRNALeu genes are expressed in the chloroplasts. The opposite strand of the tRNAArg gene contains a tRNAArg-like sequence. The tRNAArg, tRNAAsn and tRNALeu coding regions are contained in open reading frames.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421612
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  • 5
    Electronic Resource
    Electronic Resource
    High-speed electro-fusion and electro-transfection of plant protoplasts by a continuous flow electro-manipulator (1988)
    Springer
    Plant cell reports 7 (1988), S. 153-157 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A continuous flow electro-manipulator available both for mass production of fused and of transfected plant protoplasts was devised using a flow chamber with gold-coated glass panel electrodes. Up to 100 ml of protoplasts suspension were treated within 20 min at the rate of approximately 1×106 protoplasts / min. The yield of diheterokaryons between tobacco mesophyll and carrot root protoplasts reached approximately 10 % of total protoplasts by flow electro-fusion. More than 95 % of tobacco and cowpea mesophyll protoplasts became infected with tobacco mosaic virus RNA by flow electro-transfection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269311
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  • 6
    Electronic Resource
    Electronic Resource
    Beta chloroplast genomes: analysis of Fraction I protein and chloroplast DNA variation (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 330-336 
    Details
    ISSN: 1432-2242
    Keywords: Beta ; Fraction I protein ; Chloroplast DNA ; Comparative restriction site mapping ; Phylogenetic relationship
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The interrelationships of Beta chloroplast genomes have been investigated on the basis of the analysis of Fraction I protein and chloroplast (ct) DNA. Three groups of the chloroplast genomes could be demonstrated by the difference in isoelectric points of the large subunit of Fraction I protein. Restriction enzyme analysis revealed inter- and intra-specific variations among the ctDNAs, which enabled us to detect seven distinct ctDNA types. In Vulgares and Corollinae species, the observed differences were physically mapped taking advantage of the restriction fragment map available for sugar beet (B. vulgaris) ctDNA. The DNA variations were found to result either from gains or losses of restriction sites or from small deletions/ insertions, and most of them were located in the large single-copy region of the genome. Moreover, the ctDNAs from Patellares species are more diverged from those of other Beta taxa. Our results also indicate that there is a close correlation between the chloroplast genome diversity and the accepted taxonomic classification of the species included in this survey.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262497
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  • 7
    Electronic Resource
    Electronic Resource
    Rice chloroplast DNA: a physical map and the location of the genes for the large subunit of ribulose 1,5-bisphosphate carboxylase and the 32 KD photosystem II reaction center protein (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 117-122 
    Details
    ISSN: 1432-2242
    Keywords: Rice ; Chloroplast DNA ; Physical map ; Restriction pattern
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary By homogenizing rice leaves in liquid nitrogen, it was possible to isolate intact chloroplasts and, subsequently, pure rice chloroplast DNA from the purified chloroplasts. The DNA was digested by several restriction enzymes and fragments were fractionated by agarose gel electrophoresis. The sum of the fragment sizes generated by the restriction enzymes showed that the total length of the DNA is 130 kb. A circular physical map of fragments, generated by digestion with SalI, PstI, and PvuII, has been constructed. The circular DNA contains two inverted repeats of about 20 kb separated by a large, single copy region of about 75 kb and a short, single copy region of about 15 kb. The location of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase (Fraction I protein) and the 32 KD photosystem II reaction center gene were determined by using as probes tobacco chloroplast DNAs containing these genes. Rice chloroplast DNA differs from chloroplast DNAs of wheat and corn as well as from dicot chloroplast DNAs by having the 32 KD gene located 20 kb removed from the end of an inverted repeat instead of close to the end, as in other plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00275309
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  • 8
    Electronic Resource
    Electronic Resource
    Organelle genome diversity in sugar beet with normal and different sources of male sterile cytoplasms (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 166-171 
    Details
    ISSN: 1432-2242
    Keywords: Sugar beet ; Cytoplasmic male sterility ; Mitochondrial and chloroplast DNAs ; Restriction endonuclease fragment analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial (mt) and chloroplast (ct) DNAs from sugar beet lines carrying normal and introduced sources of male sterile cytoplasms have been characterized and compared on the basis of restriction enzyme analysis. Normal cytoplasm was shown to contain mt and ctDNAs which differed from those of the male sterile cytoplasms examined in the present investigation. On the other hand, four groups of male sterile cytoplasms could be differentiated by their own characteristic mtDNA digest patterns, while two were separated by ctDNA comparisons. In addition, a greater degree of variability of the mitochondrial genome is suggested. Our results also imply strict maternal inheritance of mt and ctDNAs. Thus, the organelle DNA assay provides a positive and alternative means of identifying various male sterile cytoplasms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252051
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  • 9
    Electronic Resource
    Electronic Resource
    A novel nucleic acid-binding protein in the cyanobacterium Synechococcus sp. PCC6301: a soluble 33-kDa polypeptide with high sequence similarity to ribosomal protein S1 (2000)
    Springer
    Molecular genetics and genomics 263 (2000), S. 655-663 
    Details
    ISSN: 1617-4623
    Keywords: Key words Cyanobacteria ; Ribosomal protein S1 ; Nucleic acid-binding protein ; S1 motif ; Ribosome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyanobacteria are prokaryotes that carry out plant-type photosynthesis and contain several eukaryotic-type RNA-binding proteins. Using a single-stranded DNA column, a 33-kDa protein was isolated and characterized from Synechococcus sp. PCC6301. This protein of 293 amino acids is similar in overall structure to the ribosomal protein S1 found in the same species, and contains three repeated units that are highly similar to the S1 motif originally found in the ribosomal protein S1 of Escherichia coli. However, the 33-kDa protein was found not to be associated with ribosomes and its nucleic acid binding specificity is distinct from that of the ribosomal protein S1. As this protein has high affinity for both single- and double-stranded DNA, as well as for poly(G) and poly(A), we tentatively named it nucleic acid-binding protein 1 (Nbp1).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051214
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