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  • 1985-1989  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Phospholipid and fatty acid enrichment of Phanerochaete chrysosporium INA-12 in relation to ligninase production (1988)
    Springer
    Applied microbiology and biotechnology 27 (1988), S. 393-398 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Ligninase activity of Phanerochaete chrysosporium INA-12 was increased when vegetable oils emulsified with sorbitan polyoxyethylene monooleate (Tween 80) were added to growth medium. Maximal enzyme yield was 22.0 nkat·ml-1 in olive oil cultures after 4 days incubation. P. chrysosporium INA-12 was also able to utilize tall oil fatty acids for ligninase synthesis. An extracellular lipase activity was detected during the primary phase of growth in culture containing vegetable oils. On the other hand, ligninase production was 1.5-fold enhanced when olive oil cultures were supplemented with soybean asolectin as a phospholipid source. In cultures supplied with olive oil plus asolectin, P. chrysosporium INA-12 mycelium exhibited a preferential enrichment of oleic acid (C18:1), phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) as compared to lipid-free medium. PC and LPC enrichment was associated with an increased ratio of saturated versus unsaturated fatty acids of phospholipids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251775
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Improved fermentation of cellobiose, glucose, and xylose to ethanol by Zymomonas anaerobia and a high ethanol tolerant strain of Clostridium saccharolyticum (1985)
    Springer
    Applied microbiology and biotechnology 21 (1985), S. 234-237 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary To improve single step conversion of sugar mixtures containing cellobiose, glucose, and xylose to ethanol by a coculture of Zymomonas anaerobia and Clostridium saccharolyticum, an ethanol tolerant mutant of C. saccharolyticum was obtained. The mutant obtained by the enrichment procedure was able to grow in the presence of 75 g·l-1 ethanol, with improved ability to utilize cellobiose, and little or no change in its ability to convert xylose to ethanol. This mutant in coculture with Zymomonas anaerobia produced over 50 g·l-1 ethanol in media containing 130 g·l-1 total sugars comprising of 60% glucose, 20% cellobiose, and 20% xylose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00295128
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Cellulase production by trichoderma harzianum in static and mixed solid-state fermentation reactors under nonaseptic conditions (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 27 (1985), S. 1385-1388 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cellulase production from lignocellulosic materials was studied in solid-state cultivation by both static and mixed techniques under nonaseptic conditions. The effects of fermentation conditions, such as moisture content, pH, temperature, and aeration, on cellulase production by Trichoderma harzianum using a mixture of wheat straw (80%) and bran (20%) were investigated. With a moisture content of 74% and a pH of 5.8., 18 IU filter paper activity and 198 IU endoglucanase activity/g initial substrate content were obtained in 66 h. The extension from static column cultivation to stirred tank reactor of 65 L capacity gave similar yields of cellulase.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260270917
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    Paper (German National Licenses)
    Fulltext
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