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  • 1985-1989  (6)
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Material
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Phenotypical and Functional Characterization of the Idiotype-Positive Blood B Cells in Multiple Myeloma (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 28 (1988), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In this study the idiotype-positive B cells of one patient with smouldering multiple mycloma (IgGx) and of one patient with multiple myeloma (IgGλ) were analysed phenotypically and functionally. As regards the expression of B cell-associated differentiation antigen and size distribution, the idiotype-positive B ells resembled normal IgG-bearing blood B Cells. In functional studies the lymphocytes were cultured in vitro with Staphylococcus aureus. pokeweed mitogen, T-cell factors, or combinations of those. After culture, proliferation and differentiation of the idiotype-positive B cells were measured by autoradiography, an idiotype-specific ELISA, and B spot ELISA. The results show that idiotype-positive B Cell of both patients are able to proliferate after simulation in vitro. In contrast to their normal counterparts, however, almost no increase in the amount of secreted idiotypic IgG could be induced. This suggests that the idiotype-positive blood B cells have lost some of their ability to respond to exogenous stimuli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1988.tb01513.x
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Generation of High-Rate Ovalbumin-Specific Antibody-Secreting Cells in Cultures of Human Peripheral Blood B Cells Obtained from Non-Immunized Blood Donors (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 28 (1988), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: When human peripheral him id B cells are cultured for 6 days with the T cell-dependent peptide antigen ovalbumin (OA) in the presence of antigen-presenting cells and helper T cells, plaque-forming cells (PFC) are generated. These OA-induced PFC differ from the conventional high-rate antibody-secreting PFC formed after stimulation of B cells with recall antigens (e.g. tetanus toxoid) in that they secrete antibody at a very low level. Previous studies have shown that OA-induced PFC are B lymphocytes in an early activation stale rather than cells that have differentiated into plasmablasts. The apparent arrest in the maturation of OA-induced PFC in an early activation phase can he overcome by simultaneous stimulation with interleukin 2 (1L-2) and gamma interferon (IFN-γ). The isotype of the OA-specific antibodies secreted, however, are only of the IgM class, demonstrating that an isotype switch docs not occur.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1988.tb01490.x
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Calcium Ionophore A23187 Induces Interleukin 2 Reactivity in Human T Cells (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 22 (1985), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In the present study the activation of purified human T lymphocytes by the calcium ionophore A23187 was analysed in the light of current concepts of receptor-linked inositol lipid metabolism. It was found that A23187 was only slightly mitogenic, with a narrow optimum at 400-500 nM. The proliferation could be blocked by anti-Tac ascites at 10-3 dilution, suggesting an interleukin 2 (IL-2)-dependent pathway of activation. However, an unexpectedly large proportion of A23187-stimulated cells expressed the IL-2 receptor. Reculturing the cells with exogenous IL-2 after removal of A23187 resulted in strongly enhanced proliferation. Phorbol myristic acetate (PMA) at non-mitogenic concentrations exerted an extremely strong synergistic effect on A23187-induced cell proliferation, which was, again, mediated via an IL-2-dependent pathway. Supernatants of A23187-stimulated T cells did not contain detectable amounts of IL-2. Combination of PMA and A23187 resulted in considerable IL-2 production. It is concluded that A23187 induces the expression of IL-2 receptors without concurrent stimulation of IL-2 production, thus allowing only low levels of proliferation. Addition of exogenous IL-2 or of PMA restores the imbalance between the occurrence of IL-2 and its receptor and results in high rates of proliferation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1985.tb01925.x
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Ligands of Surface Ig Raise Cytoplasmic Free Ca++ in Human B Cells (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 22 (1985), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: With the use of the fluorescent Ca++ indicator Quin-2, we have measured changes in intracellular calcium levels in human B cells in response to anti-Ig antibodies, to Staphylococcus aureus (Staph) or to protein A. Cells of an Epstein-Barr virus-transformed μλ-carrying B-cell line, AZU-1, increased free cytosolic calcium after addition of anti-μ or anti-μ antibodies: F (ab′)2 fragments with anti-μ specificity were equally effective. Fab fragments of sheep anti-Ig antibodies only induced a rise in calcium levels after addition of a second anti-sheep Ig antiserum. Cross-linking of non-Ig surface determinants did not influence calcium homeostasis. The calcium channel blockers verapamil (100 μM). nifedipine (20 μM). and LaCl3 (200 μM) inhibited the anti-μ-induced calcium influx. Peripheral blood B cells reacted in essentially the same way in response to anti-μ antibodies. The B cell mitogens protein A and Staph also induced a rise in intracellular calcium. These observations indicate that Ca++ may play a role as a messenger in the activation of human B cells via surface Ig.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1985.tb01915.x
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Ovalbumin-Specific Human B-Cell Activation and Maturation (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 28 (1988), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: By means of a panel of monoclonal antibodies it is demonstrated that, in cultures of human peripheral blood mononuclear cells (PBMC) with the T-cell dependent (TD) antigen ovalbumin (OA), responding B ceils are activated from the resting Mate The differentiation of the activated B cells to high rate-secreting plasma blasts, however, is attested in an early activation phase, in which they can be detected at low rate-secreting plaque-forming cell. The arrest does not occur when stimulation with OA occurs in the presence of antigen-nonspecific activation and maturation factors, which are provided in the culture by the anamnestic response to the TD antigen tetanus toxoid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1988.tb02414.x
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    The Impact of Mild Emotional Stress Induced by the Passive Avoidance Procedure on Immune Reactivity (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 496 (1987), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1987.tb35804.x
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    Paper (German National Licenses)
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