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  • 1
    Electronic Resource
    Electronic Resource
    Human cystic kidney diseases: epithelial hyperplasia in the pathogenesis of cysts and tumors (1987)
    Springer
    Pediatric nephrology 1 (1987), S. 393-396 
    Details
    ISSN: 1432-198X
    Keywords: Polycystic kidney disease ; Renal carcinoma ; Tuberous sclerosis ; von Hippel-Lindau disease ; Acquired renal cystic disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Several examples of human renal cystic disease are associated with tubular epithelial hyperplasia. Micropapillary hyperplasia occurs in autosomal dominant polycystic kidney disease, in localized cystic disease, and in acquired cystic disease; neoplastic or severely dysplastic epithelial hyperplasia occurs in von Hippel-Lindau disease; a histopathologically distinctive epithelial hyperplasia occurs in tuberous sclerosis. In all of these conditions the epithelial hyperplasia appears to be responsible for cyst formation by causing tubular or ductal luminal obstruction, and in all of these conditions, save localized cystic disease (a rare condition with very few reported cases), epithelial hyperplasia imposes an increased risk of malignancy. The risk seems to be highest in patients under treatment with long-term hemodialysis for end-stage kidney disease. Some of these diseases may share common features, but it appears likely that the histopathological differences reflect different features converging on a common result.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00849242
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  • 2
    Electronic Resource
    Electronic Resource
    Postnatal maturation of the rabbit cortical collecting duct (1988)
    Springer
    Pediatric nephrology 2 (1988), S. 135-145 
    Details
    ISSN: 1432-198X
    Keywords: Cortical collecting duct ; Potassium ; Mitosis ; Helium glow photometry ; Quartz fiber balance ; Scanning electron microscopy ; Transmission electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The mature, fully differentiated cortical collecting duct plays a major role in the final renal regulation of Na+, K+ and H+ transport. To characterize the growth of this segment, we measured the outer diameter and the dry weight of cortical collecting ducts isolated from newborn, 1-month-old, and adult rabbits. During the 1st month of life no significant changes were observed; however, there was a 60% increase in both parameters after the 4th week of life. Growth-related accretion of K+ was demonstrated by showing tubular K+ content to increase by 60% with maturation. Concomitant with the increase in tubular size, total cell number per millimeter of tubular length rose by 30%. Approximately 50% of the observed increment in tubular size could be accounted for by cell hyperplasia, with the remaining increase resulting from cell hypertrophy. Hypertrophy of principal cells was confirmed by scanning electron microscopy, which demonstrated a doubling of the circumferential width without any change in longitudinal length. Hyperplasia was confirmed, using a fluorescent chromatin stain, by our finding of a mitotic frequency of 3/1000 cells in the neonatal mid-cortical collecting duct; the observed number of mitoses was 10-fold higher at the most cortical end (ampulla). The number of intercalated cells per millimeter of tubule length, identified by bright green fluorescence after cortical collecting ducts were stained with 6-carboxyfluorescein diacetate, was found to double during maturation, the increase being significant only after the 4th postnatal week. We conclude that maturation of the mid-cortical collecting duct results from both cellular hyperplasia and hypertrophy. It is unlikely that this segment plays a major role in regulating Na+, K+, and H+ transport in the neonatal kidney.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00870394
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  • 3
    Electronic Resource
    Electronic Resource
    MDCK cysts: An in vitro model of epithelial cyst formation and growth (1986)
    Springer
    Methods in cell science 10 (1986), S. 245-248 
    Details
    ISSN: 1573-0603
    Keywords: MDCK cell ; renal cell culture ; epithelial cysts ; collagen gel ; clonal growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary MDCK cells dissociated from monolayer culture were either dispersed within medium-hydrated collagen gel or seeded atop a collagen substrate which was immediately overlaid with collagen gel. Individual cells exhibited clonal growth in three dimensions to form spherical cysts in which a simple epithelium surrounded a fluid-filled lumen. The cells of MDCK cysts were polarized with apical surface bordering the lumen. MDCK cysts increased in diameter with continued culture. Maximum cyst size was dependent on seeding density and was influenced by medium composition. MDCK cysts could be isolated from the collagen substrate by digestion with collagenase. Also, collagen gel could be dissected from the cyst wall to give unrestricted access to regions of the basolateral cell surface. This novel method of renal cell culture provides a study system to model the influence of the extracellular matrix on kidney epithelial cell structure and function. It also offers an in vitro model of general application to the study of epithelial cyst formation and growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404485
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  • 4
    Electronic Resource
    Electronic Resource
    Morphogenetic clonal growth of kidney epithelial cell line MDCK (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 217 (1987), S. 229-239 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: MDCK (Madin-Darby canine kidney) cells were cultured either (1) dispersed within hydrated collagen gel (HCG) or (2) seeded atop a collagen substrate and then immediately overlaid with HCG. Individual cells exhibited clonal growth in three dimensions to form spherical cysts made up of a simple epithelium enclosing a fluid-filled lumen. The cells of MDCK cysts were polarized with the basolateral surface in contact with the collagen gel and the apical surface bordering the lumen. The ultrastructure of MDCK cysts showed similarities to distal nephron. The cells bore apical microvilli and solitary cilia and had occluding junctions and a simple basolateral surface. MDCK cysts increased in size (〉 800 μm diameter) with continued culture. MDCK cysts grown between layers of HCG were stripped free of the overlying collagen to give direct access to basolateral surface membrane. Unlike monolayer culture, morphogenetic clonal growth of cell line MDCK produces a polarized cell population with a true lumenal and basolateral surface. Collagen-gel-cultured MDCK cysts provide an easily manipulable in vitro cell system that may offer unique advantages for the study of renal cell structure and function.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092170303
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  • 5
    Electronic Resource
    Electronic Resource
    Morphological evaluation of vascular smooth muscle cell: Length and width from a single scanning electron micrograph of microvessels (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 216 (1986), S. 95-103 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Accurate three-dimensional data on the structure of vascular smooth muscle cells is essential for understanding the microvascular system in both normal or disease conditions. The laborious serial reconstruction methods have limited the amount of data collected on the structure of individual vascular smooth muscle (VSM) cells. The circumferential viewing of whole vessel segments via scanning electron microscopy provides an alternative approach, but even this technique is highly specialized and tedious. This study presents a simplified method to determine the average cell length and width of individual VSM cells by using only one view of a microvessel (single view). The vessels do not have to be isolated for circumferential viewing and can be left in their host tissue if desired. Values for the average VSM cell length and width were obtained by both circumferential- and single-view approach on the same vessels. The average cell length and width obtained from the single-view method (using one-third circumference) duplicated the mean length and width measurements obtained by circumferential viewing.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092160116
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Microvascular smooth muscle cell quantitation from scanning electron microscopic preparations (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 216 (1986), S. 443-447 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A morphometric method to analyze scanning electron micrographs (SEM) of microvascular smooth muscle cells (SMC) is validated using intestinal arterioles. Features easily obtained from these microvasculature preparations are counted (number of tapers and number of complete wraps in the central 87% of the vessel, which is one-third of the vessel's circumference) or measured (vessel diameter and vessel segment length). These data allow the determination of wraps around the vessel per SMC, cell length, and cell width, which are not different from either the values as determined by circumferential examination and quantitation of the vascular segment or an alternative SEM quantitation method (Miller et al., 1986). The method described herein provides a relatively easy way to determine microvascular SMC parameters.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092160315
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