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  • 1
    Electronic Resource
    Electronic Resource
    Calcium oxalate urolithiasis in mice lacking anion transporter Slc26a6 (2006)
    [s.l.] : Nature Publishing Group
    Nature genetics 38 (2006), S. 474-478 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Urolithiasis is one of the most common urologic diseases in industrialized societies. Calcium oxalate is the predominant component in 70–80% of kidney stones, and small changes in urinary oxalate concentration affect the risk of stone formation. SLC26A6 is an anion exchanger expressed on the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng1762
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  • 2
    Electronic Resource
    Electronic Resource
    Postnatal maturation of the rabbit cortical collecting duct (1988)
    Springer
    Pediatric nephrology 2 (1988), S. 135-145 
    Details
    ISSN: 1432-198X
    Keywords: Cortical collecting duct ; Potassium ; Mitosis ; Helium glow photometry ; Quartz fiber balance ; Scanning electron microscopy ; Transmission electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The mature, fully differentiated cortical collecting duct plays a major role in the final renal regulation of Na+, K+ and H+ transport. To characterize the growth of this segment, we measured the outer diameter and the dry weight of cortical collecting ducts isolated from newborn, 1-month-old, and adult rabbits. During the 1st month of life no significant changes were observed; however, there was a 60% increase in both parameters after the 4th week of life. Growth-related accretion of K+ was demonstrated by showing tubular K+ content to increase by 60% with maturation. Concomitant with the increase in tubular size, total cell number per millimeter of tubular length rose by 30%. Approximately 50% of the observed increment in tubular size could be accounted for by cell hyperplasia, with the remaining increase resulting from cell hypertrophy. Hypertrophy of principal cells was confirmed by scanning electron microscopy, which demonstrated a doubling of the circumferential width without any change in longitudinal length. Hyperplasia was confirmed, using a fluorescent chromatin stain, by our finding of a mitotic frequency of 3/1000 cells in the neonatal mid-cortical collecting duct; the observed number of mitoses was 10-fold higher at the most cortical end (ampulla). The number of intercalated cells per millimeter of tubule length, identified by bright green fluorescence after cortical collecting ducts were stained with 6-carboxyfluorescein diacetate, was found to double during maturation, the increase being significant only after the 4th postnatal week. We conclude that maturation of the mid-cortical collecting duct results from both cellular hyperplasia and hypertrophy. It is unlikely that this segment plays a major role in regulating Na+, K+, and H+ transport in the neonatal kidney.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00870394
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  • 3
    Electronic Resource
    Electronic Resource
    Human cystic kidney diseases: epithelial hyperplasia in the pathogenesis of cysts and tumors (1987)
    Springer
    Pediatric nephrology 1 (1987), S. 393-396 
    Details
    ISSN: 1432-198X
    Keywords: Polycystic kidney disease ; Renal carcinoma ; Tuberous sclerosis ; von Hippel-Lindau disease ; Acquired renal cystic disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Several examples of human renal cystic disease are associated with tubular epithelial hyperplasia. Micropapillary hyperplasia occurs in autosomal dominant polycystic kidney disease, in localized cystic disease, and in acquired cystic disease; neoplastic or severely dysplastic epithelial hyperplasia occurs in von Hippel-Lindau disease; a histopathologically distinctive epithelial hyperplasia occurs in tuberous sclerosis. In all of these conditions the epithelial hyperplasia appears to be responsible for cyst formation by causing tubular or ductal luminal obstruction, and in all of these conditions, save localized cystic disease (a rare condition with very few reported cases), epithelial hyperplasia imposes an increased risk of malignancy. The risk seems to be highest in patients under treatment with long-term hemodialysis for end-stage kidney disease. Some of these diseases may share common features, but it appears likely that the histopathological differences reflect different features converging on a common result.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00849242
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  • 4
    Electronic Resource
    Electronic Resource
    MDCK cysts: An in vitro model of epithelial cyst formation and growth (1986)
    Springer
    Methods in cell science 10 (1986), S. 245-248 
    Details
    ISSN: 1573-0603
    Keywords: MDCK cell ; renal cell culture ; epithelial cysts ; collagen gel ; clonal growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary MDCK cells dissociated from monolayer culture were either dispersed within medium-hydrated collagen gel or seeded atop a collagen substrate which was immediately overlaid with collagen gel. Individual cells exhibited clonal growth in three dimensions to form spherical cysts in which a simple epithelium surrounded a fluid-filled lumen. The cells of MDCK cysts were polarized with apical surface bordering the lumen. MDCK cysts increased in diameter with continued culture. Maximum cyst size was dependent on seeding density and was influenced by medium composition. MDCK cysts could be isolated from the collagen substrate by digestion with collagenase. Also, collagen gel could be dissected from the cyst wall to give unrestricted access to regions of the basolateral cell surface. This novel method of renal cell culture provides a study system to model the influence of the extracellular matrix on kidney epithelial cell structure and function. It also offers an in vitro model of general application to the study of epithelial cyst formation and growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404485
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  • 5
    Electronic Resource
    Electronic Resource
    An acoustically matched high pressure chamber for control of cavitation in shock wave lithotripsy: Mechanisms of shock wave damage in vitro (1998)
    Springer
    Methods in cell science 19 (1998), S. 303-310 
    Details
    ISSN: 1573-0603
    Keywords: Cavitation ; Cell injury ; Shock wave lithotripsy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Increased ambient pressure (excess hydrostatic pressure) was used to regulate cavitation in the aqueous media surrounding isolated red blood cells and aluminum foils used as targets in studies of the mechanisms of shock wave (SW) damage in shock wave lithotripsy (SWL). Foils or cells were placed in a cylindrical chamber (bronze-aluminum alloy) connected to a regulator and nitrogen source. The ends of the cylinder were capped with planar, plastic plates 12.7 mm thick. Tests performed with a PVDF membrane hydrophone showed that SW's passed through the plastic without significant loss of amplitude or change in waveform. Pitting of foils, a form of damage that can only be due to cavitation, was eliminated by very high (∼70 atm) added pressure. It took substantially greater added pressure to reduce damage to foils than has been reported to reduce damage to cells, suggesting differences in how cavitation is regulated in bulk fluid versus a solid surface. This report describes the design of the pressure chamber used in these studies and explains how SW exposures of isolated cells and foil targets were performed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009799222889
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  • 6
    Electronic Resource
    Electronic Resource
    Culture of human renal cortex epithelial cells (1991)
    Springer
    Methods in cell science 13 (1991), S. 143-147 
    Details
    ISSN: 1573-0603
    Keywords: kidney ; renal cortex ; proximal tubule ; enzymatic dissociation ; cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A procedure is described for the establishment and propagation of epithelial cell rich cultures derived from normal human kidney cortex (NHK-C cells). Cells are harvested from tissue fragments of donor human kidney by progressive enzymatic dissociation. NHK-C cultures are morphologically heterogeneous but exhibit, predominantly, the functional characteristics of cells of the kidney proximal tubule.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02388118
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  • 7
    Electronic Resource
    Electronic Resource
    A method for processing free-floating cultured cells for scanning electron microscopy (1982)
    Springer
    Methods in cell science 7 (1982), S. 85-88 
    Details
    ISSN: 1573-0603
    Keywords: specimen processing ; scanning electron microscopy ; aggregate cultures ; isolated cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A simple and convenient method is described to aid the processing of minute tissue specimens and isolated single cells for scanning electron microscopy (SEM). The technique employs an inexpensive, disposable carrier fashioned from common Beem capsules and Nitex nylon screen. This method avoids excessive handling, transfers or centrifugation steps that may damage delicate specimens. Specimens processed by this method show excellent preservation of cell surface fine structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01665947
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  • 8
    Electronic Resource
    Electronic Resource
    Submersion organ culture of preglandular fetal lung (1983)
    Springer
    Methods in cell science 8 (1983), S. 59-62 
    Details
    ISSN: 1573-0603
    Keywords: organ culture ; fetal rat lung ; submersion culture ; hydrated collagen gel ; pulmonary epithelium ; cytodifferentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This report describes a method for organ culture of the fetal rat lung at the early preglandular phase of development. When the intact fetal lung is cultured fully submerged in medium it develops as an expanded hollow organ. This pattern of in vitro morphogenesis provides a specimen uniquely suited to analysis of the pulmonary epithelium by scanning electron microscopy. In this system alveolar Type II cells and cell types of the distal pulmonary airways undergo differentiation in serum-free media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02099239
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  • 9
    Electronic Resource
    Electronic Resource
    Effects of chronic deafferentation on adrenergic ganglion cells and small intensely fluorescent cells (1978)
    Springer
    Journal of neurocytology 7 (1978), S. 25-37 
    Details
    ISSN: 1573-7381
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To determine the reaction of adrenergic ganglion cells and small intensely fluorescent (SIF) cells to chronic deafferentation, catecholamine fluorescence of the major pelvic ganglion (MPG) of the rat has been studied following section of the hypogastric nerve, pelvic nerve and sympathetic trunk. Only minor changes occurred following section of the hypogastric nerve; the fluorescence surrounding a few adrenergic ganglion cells became brighter. In contrast, pelvic neurectomy resulted in the appearance of numerous varicose fibres and an increase in the fluorescent intensity of fibres enclosing many ganglion cells. Varicose fibres seem to originate from adrenergic ganglion cells and SIF cells. In many instances, nests of SIF cells gave rise to radially oriented fibres. Removal of the sympathetic trunk appeared to have no effect on the MPG. It is suggested that the appearance of varicose fibres from SIF cells following deafferentation may be due to collateral sprouting of these cells or to the increased fluorescence of pre-existing processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01213458
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  • 10
    Electronic Resource
    Electronic Resource
    Ultrastructure of the developing vascular system in the puppy kidney (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 199 (1981), S. 481-489 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The present study defined the ultrastructural features of peritubular capillary development. Two-day-old beagle puppies and adult dogs were perfused with 2.5% glutaraldehyde and routinely prepared for light and transmission electron microscopy. Some of the fixed tissue was subsequently used to make freeze-fracture replicas.The outer cortex of the puppy kidney possessed large, thick-walled vessels best termed sinusoidal capillaries instead of the small caliber vessels (peritubular capillaries) noted in the adult. These sinusoidal vessels showed extensive overlapping of the endothelium with isolated patches of fenestrae. Their luminal surfaces were irregular, owing to prominent ridges and sporadic bulges of endothelium. The basement membrane of most vessels was not present. Interstitial spaces were filled with mesenchymal cells and cells closely resembling pericytes. The diameter of the fenestrae of vessels throughout the cortex was similar; however, the number of fenestrae per micrometer of endothelium increased significantly from outer to inner cortex. Vessels of the inner cortex were also immature when compared to the adult. From these morphological findings, it was apparent that a true peritubular capillary system does not exist in the two-day-old puppy. Ultrastructural features of these vessels suggested reduced permeability characteristics.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091990405
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