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Culture of human renal cortex epithelial cells (1991)

McAteer, James A. ; Kempson, Stephen A. ; Evan, Andrew P.

Springer

Methods in cell science 13 (1991), S. 143-147

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ISSN: 1573-0603

Keywords: kidney ; renal cortex ; proximal tubule ; enzymatic dissociation ; cell culture

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary A procedure is described for the establishment and propagation of epithelial cell rich cultures derived from normal human kidney cortex (NHK-C cells). Cells are harvested from tissue fragments of donor human kidney by progressive enzymatic dissociation. NHK-C cultures are morphologically heterogeneous but exhibit, predominantly, the functional characteristics of cells of the kidney proximal tubule.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02388118

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