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Submersion organ culture of preglandular fetal lung (1983)

McAteer, James A. ; Cavanagh, Thomas J. ; Evan, Andrew P.

Springer

Methods in cell science 8 (1983), S. 59-62

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ISSN: 1573-0603

Keywords: organ culture ; fetal rat lung ; submersion culture ; hydrated collagen gel ; pulmonary epithelium ; cytodifferentiation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary This report describes a method for organ culture of the fetal rat lung at the early preglandular phase of development. When the intact fetal lung is cultured fully submerged in medium it develops as an expanded hollow organ. This pattern of in vitro morphogenesis provides a specimen uniquely suited to analysis of the pulmonary epithelium by scanning electron microscopy. In this system alveolar Type II cells and cell types of the distal pulmonary airways undergo differentiation in serum-free media.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02099239

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