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  • 1
    Electronic Resource
    Electronic Resource
    Versatile photostimulated exoelectron microscope with measurement capabilities in SEM mode (1985)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 56 (1985), S. 1934-1936 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: Photostimulated exoelectron microscope (PSEM) for observing mechanically damaged surfaces has been developed, which enables one to observe a photostimulated exoelectron (PSE) image in an EXO mode and the corresponding secondary electron image of the same region in a SEM mode, without reassembling an electron optical system. Typical results obtained with this microscope are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1138448
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  • 2
    Electronic Resource
    Electronic Resource
    The existance of rhabdoid cells in specified soft tissue sarcomas (1987)
    Springer
    Virchows Archiv 411 (1987), S. 509-514 
    Details
    ISSN: 1432-2307
    Keywords: Soft tissue neoplasm ; Sarcoma ; Rhabdoid cell ; Rhabdoid tumor ; Ultrastructure ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We report the occurrence of rhabdoid cells in several specified soft tissue sarcomas of round cell variety. The rhabdoid cells had an acidophilic cytoplasm containing a globular perinuclear inclusion and were characterised ultrastructurally by the presence of aggregates of 10 nm intermediate filaments. These filaments contained both cytokeratin and vimentin, as demonstrated immunohistochemically. Extensive sampling of soft tissue sarcomas revealed the presence of such cells in different types of soft tissue round cell sarcomas as follows: 12 of 13 cases of epithelioid sarcomas, 8 of 13 synovial sarcomas (composed predominantly of round cells), 6 of 20 extraskeletal myxoid chondrosarcomas and 4 of 4 round celled malignant mesotheliomas. We wish to stress that the appearance of rhabdoid cells is not a monopoly of one particular type of tumour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00713281
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  • 3
    Electronic Resource
    Electronic Resource
    In situ hybridization analysis of cytomegalovirus lytic infection in Kaposi's sarcoma associated with AIDS (1987)
    Springer
    Virchows Archiv 411 (1987), S. 441-448 
    Details
    ISSN: 1432-2307
    Keywords: Kaposi's sarcoma ; Cytomegalovirus ; In situ hybridization ; Autopsy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cytomegalovirus (CMV) was assayed by in situ hybridization with commercially available biotin-labeled CMV-DNA probes in 45 formalin-fixed paraffin-embedded autopsy specimens with Kaposi's sarcoma from 14 cases of the acquired immune deficiency syndrome (AIDS). In seven of the 14 cases, a few scattered hybridizing cells were detected in Kaposi's sarcoma, but not all specimens from the same case showed such cells. Most of the positive cells were peculiarly swollen and not typical of Kaposi's sarcoma cells. All positive cases had at least some CMV-infected organs with typical cytomegalic cells containing nuclear inclusions while five of the 7 negative cases revealed no CMV-infected tissue by conventional light microscopy. Our results suggest that this in situ hybridization procedure using biotin-labeled DNA probes only reveals generalized CMV infection that is a consequence of impairment of immune mechanisms in AIDS patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00735225
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  • 4
    Electronic Resource
    Electronic Resource
    Evaluation of endoscopic ultrasonography for gastric tumors and presentation of three-dimensional display of endoscopic ultrasonography (1989)
    Springer
    Surgical endoscopy and other interventional techniques 3 (1989), S. 173-181 
    Details
    ISSN: 1432-2218
    Keywords: Endoscopic ultrasonography ; Extragastric mural compression ; Gastric submucosal tumors ; Serosal invasion ; Gastric malignant tumor ; Three-dimensional display
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We have studied the usefulness of endoscopic ultrasonography (EUS) for: (1) differentiation between extragastric mural compression (EGMC) and submucosal tumors (SMT); (2) qualitative assessment of these lesions; (3) diagnosis of serosal invasion by malignant gastric tumors; (4) presentation of three-dimensional display of EUS findings. A total of 103 patients with submucosal lesions protruding into the gastric lumen on endoscopy were examined. Differentiation between EGMC and SMT could be made by EUS in all cases. Qualitative assessment of these lesions was made by observing their EUS findings, i.e. their internal and marginal echo pattern and their location in the gastric layers. The correct diagnosis was made in 88% (23/26) of EGMC. Of 50 patients with SMT, comprising myogenic tumor (32), aberrant pancreas (8), lipoma (3), gastric cyst (4) and malignant lymphoma (elevated type; 3), differentiation between malignant and benign myogenic tumors was impossible by EUS alone. In addition, 48 patients with advanced gastric cancer and malignant lymphoma were examined. Invasion to the serosal surface was diagnosed as interruption of the fifth layer of the gastric wall. Invasion to other organs was diagnosed by interruption and fusion of the fifth layer into the border of the adjacent structure. Serosal invasion was diagnosed accurately in 65% of 48 patients. Microscopic invasion was difficult to diagnose, and gastric stenosis by tumor was an obstacle to adequate scanning. EUS can be valuable in the differential diagnosis between EGMC and SMT and in the qualitative diagnosis of gastric lesions. It is also effective in detecting serosal invasion by gastric malignant tumors. Three-dimensional display of pathological lesions is new in EUS. With this method, it is easy to get the stereograph and to obtain a complete picture of the lesions. We present two cases in this report.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02171541
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  • 5
    Electronic Resource
    Electronic Resource
    The Leu-1 B-cell subpopulation in patients with rheumatoid arthritis (1987)
    Springer
    Journal of clinical immunology 7 (1987), S. 441-448 
    Details
    ISSN: 1573-2592
    Keywords: Leu-1 B cells ; rheumatoid arthritis ; rheumatoid factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Ly-1- B cells have been shown to be elevated in autoimmune mice, especially NZB, and to secrete IgM autoantibody, suggesting that Ly-1 B cells may participate in autoimmune diseases. In this study, B-cell populations carrying Leu-1, a human T-cell surface molecule homologous to mouse Ly-1 (Leu-1 B), were examined by dual fluorescence flow cytometry, in peripheral blood lymphocytes taken from patients suffering from various autoimmune disease. These B cells were shown to be present at a significantly high percentage in rheumatoid arthritis (RA) patients. There was no significant correlation among the incidence of Leu-1 B cells, rheumatoid factor (RF) titers, and the amount of IgM productionin vitro. There was, however, a significantly high incidence of Leu-1 B cells in RA patients with a high RF titer (greater than 5 × 213), suggesting that these B cells in RA may play an important role in IgM RF productionin vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00915053
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  • 6
    Electronic Resource
    Electronic Resource
    Incidence of anti-DNA idiotype-positive cells in human peripheral blood (1989)
    Springer
    Journal of clinical immunology 9 (1989), S. 313-321 
    Details
    ISSN: 1573-2592
    Keywords: Anti-DNA antibody ; idiotypes ; lymphocytes ; systemic lupus erythematosus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Human anti-DNA idiotype (Id)-bearing cells in peripheral blood were sought using mouse monoclonal anti-Id antibodies to human monoclonal anti-DNA antibodies. Pretreatment with acid pH or pronase P or preincubation in human serum-free medium markedly decreased the number of anti-Id-reactive cells. Pronase P-treated cells were able to bind to anti-Id once more after incubation for 18 hr at 37° C, indicating the resynthesis of internal idiotypic determinants on the cells. Antiidiotype-reactive cells retained the same idiotypes in their cytoplasma. The cells expressing anti-DNA idiotypes, termed 0-81 and NE-1, were detected in the circulation of most patients with active lupus nephritis but not in those of inactive systemic lupus erythematosus (SLE) and healthy subjects. The idiotype-positive cells occurred in up to 5–10% of B cells from some patients with SLE in the active stage but became undetectable in remission. A limited number of anti-DNA Id-positive cells responsible for anti-DNA production might be preferentially expanded during acute episodes of the disease in some patients with SLE.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00918663
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  • 7
    Electronic Resource
    Electronic Resource
    Molecular cloning and complete nucleotide sequence of the genome of Japanese encephalitis virus Beijing-1 strain (1988)
    Springer
    Virus genes 1 (1988), S. 305-317 
    Details
    ISSN: 1572-994X
    Keywords: JEV genome ; molecular cloning, genome sequence ; translation strategy ; genome organization ; secondary structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The genomic RNA of the Japanese encephalitis virus (JEV) Beijing-1 strain was reversely transcribed and the synthesized cDNA was molecularly cloned. Six continuous cDNA clones that cover the entire virus genome were established and sequenced to determine the complete nucleotide sequence of the JEV RNA. The precise genomic size was estimated as 10,965 bases long. With flanking 95 bases at the 5′ and 583 bases at the 3′ non-coding regions, one long open reading frame (ORF) was revealed encoding a virus polyprotein with 3,429 amino acid residues. Because of sequence homologies observed between JEV and other flaviviruses, the genome organization of JEV appears to be identical with other flaviviruses. Genetic variation detected among flavivirus genomes is consistent with the established serological relatedness between JEV and other members of flaviviruses. The secondary structure of the JEV genome is deduced and discussed concerning its involvement in genome replication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00572709
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