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  • 1
    Electronic Resource
    Electronic Resource
    Specific radioimmunoassay of glucitol-lysine — application to lens proteins in streptozotocin-diabetic rats (1988)
    Springer
    Diabetologia 31 (1988), S. 221-224 
    Details
    ISSN: 1432-0428
    Keywords: Glycation ; diabetes ; radioimmunoassay ; glucitollysine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A radioimmunoassay using antibody against glucitol-lysine was developed to quantitate glycated proteins in the lens of diabetic rats. The amount of glycated protein was expressed as molar equivalents of reduced glycated hippuryl lysine (GlcRED-Hip-Lysine). Significant differences (p〈0.01) were found in the amounts of glycated protein in the lenses of rats with streptozotocin-induced diabetes (3.92±0.59 nmol/mg protein, n=5), those with streptozotocin-induced diabetes treated with insulin (2.94±0.36 nmol/mg protein, n=4) and normal rats (1.23±0.22 nmol/mg protein, n=5). There was a significant correlation between the concentration of glycated protein in the lens and the HbA1c level at the end of the 12 week experiment (r=0.957, p〈0.001). These results indicate that glycation of lens protein is parallel with the severity of diabetes in rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290588
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of the enteroinsular axis on both the A- and B-cell response to arginine after oral glucose in man (1987)
    Springer
    Diabetologia 30 (1987), S. 846-850 
    Details
    ISSN: 1432-0428
    Keywords: Enteroinsular axis ; insulin ; glucagon ; arginine ; arterial blood glucose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Studies were made on the effect of the enteroinsular axis on amino acid-induced insulin and glucagon secretion during hyperglycaemia in man. The responses of plasma immunoreactive insulin, C-peptide, and immunoreactive glucagon to arginine infusion were investigated in nine healthy subjects after induction of hyperglycaemia by an oral glucose load and by intravenous glucose infusion to produce similar glucose concentrations in the arterialised blood. The plasma immunoreactive insulin and C-peptide levels increased to higher levels after an oral glucose load than after an intravenous infusion of glucose. The incremental areas under the immunoreactive insulin and C-peptide curves during arginine infusion were significantly greater (p〈0.01) after oral than after intravenous glucose administration. The plasma immunoreactive glucagon level was suppressed equally after oral and intravenous glucose loads. However, during subsequent arginine infusion, the plasma immunoreactive glucagon level rose more in the presence of hyperglycaemia induced by oral than intravenous glucose. The incremental area under the plasma immunoreactive glucagon curve during arginine infusion was 1.6-fold greater after glucose ingestion than after intravenous glucose infusion. These results suggest that the enteroinsular axis has a stimulatory effect on the responses of pancreatic A and B cells to arginine after oral glucose administration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274792
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    High-performance liquid chromatographic assay of serum glycated albumin (1988)
    Springer
    Diabetologia 31 (1988), S. 627-631 
    Details
    ISSN: 1432-0428
    Keywords: Affinity chromatography ; anion exchange chromatography ; fructosamine ; haemoglobin A1c ; glycaemic control ; glycated protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A method for determination of serum glycated albumin by high-performance liquid chromatography is presented. The system involves anion exchange chromatography to separate albumin and consecutive boronate affinity chromatography to separate glycated and nonglycated albumin. The method is rapid (20 min), precise (coefficient of variation, 0.7–4.9%), requires only a small sample (5 μl), and can be automated. Assay of glycated albumin by this method is not influenced by the protein concentration of the sample or the presence of glucose. The variation in glycated albumin values in consecutive samples obtained within a day from diabetic patients (coefficient of variation, 2.02±0.65%) was significantly smaller (p〈0.001) than that of values for fructosamine (coefficient of variation, 4.33±2.0%). The values of glycated albumin in normal subjects (20.2±1.6%) were clearly less than those in diabetic patients [39,6±5.4% in 40 Type 1 (insulin-dependent) and 39.4±5.9% in 25 Type 2 (non-insulin-dependent) patients]. The serum glycated albumin level was well correlated with HbA1c in 65 diabetic patients (r=0.60). Because the life span of albumin in the circulation is short, measurement of glycated albumin should be useful as a short-term index of glycaemic control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264772
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The characterization and distribution of aerosol and gaseous species in the winter monsoon over the western pacific ocean (1986)
    Springer
    Journal of atmospheric chemistry 4 (1986), S. 343-358 
    Details
    ISSN: 1573-0662
    Keywords: Long-range transport of atmospheric species ; Marine aerosol and gas ; western Pacific Ocean ; aerosol characterization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract During the winters of 1981 and 1982, measurements were taken on two Japanese islands of the aerosol and gaseous species which had been carried by northwesterly monsoons over the Pacific Ocean. The aerosols were characterized as sea-salt particles; soil particles, and as particles of sulfate, nitrate, organics and elemental carbon. At Chichi-jima island, which is about 800 km away from the main islands of Japan, it was found that the level of the anthropogenic components of the aerosols was considerably higher than their background level. The mean concentrations of the species on the islands are given in tabular forms. According to a survey made on board a ferry boat, the aerosol and gaseous species were fairly uniformly distributed along the southern coast of the main Japanese islands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00053809
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  • 5
    Electronic Resource
    Electronic Resource
    Tropospheric ozone and oxides of nitrogen over the north-western Pacific in summer (1988)
    Springer
    Journal of atmospheric chemistry 6 (1988), S. 235-250 
    Details
    ISSN: 1573-0662
    Keywords: Ozone ; oxides of nitrogen ; H2O ; troposphere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract In summer, atmospheric ozone was measured from an aircraft platform simultaneously with nitric oxide (NO), oxides of nitrogen (NO y ), and water vapor over the Pacific Ocean in east Asia from 34° N to 19° N along the longitude of 138±3°E. NO y was measured with the aid of a ferrous sulfate converter. The altitude covered was from 0.5 to 5 km. A good correlation in the smoothed meridional distributions between ozone and NO y was seen. In particular, north of 25° N, ozone and NO y mixing ratios were considerably higher than those observed in tropical marine air south of 25° N. NO y and O3 reached a minimum of 50 pptv and 4 ppbv respectively in the boundary layer at a latitude of 20° N. The NO concentration between 2 and 5 km at the same latitude was 30 pptv. The profiles of ozone and water vapor mixing ratios were highly anti-correlated between 25° N and 20° N. In contrast, it was much poorer at the latitude of 33° N, suggesting a net photochemical production of ozone there.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00053858
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    Paper (German National Licenses)
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