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  • 1
    Electronic Resource
    Electronic Resource
    Serotonin reduction in the mouse neostriatum during hyperthermia-induced convulsions studied by immunohistochemistry (1988)
    Springer
    Acta neuropathologica 75 (1988), S. 541-546 
    Details
    ISSN: 1432-0533
    Keywords: Serotonin ; Mouse ; Neostriatum ; Seizure ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Changes occurring in serotonin neurons during hyperthermia-induced convulsions were examined by means of a modified immunohistochemical method. All mice (8–12 weeks of age) exposed to the temperature of 50°C had convulsions, showing a generalized tonic and/or clonic pattern. Immediately after the convulsions, the animals were perfused transcardially with a fixative. A significant reduction in serotonin immunoreactivity was observed in the neostratum (caudate-putamen complex) of the mice which had hyperthermia-induced seizures, while the serotonin immunoreactivity remained unchanged in the neocortex and paleostriatum. These results suggest that serotonin may be an important mediator in the mechanism of hyperthermia-induced convulsions or that the susceptibility of serotonin neurons to a convulsive state is greatest in the neostriatum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00686197
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural and immunohistochemical studies on the zona-reticularis cells of the adrenal cortex of normal and 3-methylcholanthrene-treated mice (1985)
    Springer
    Histochemistry and cell biology 83 (1985), S. 207-211 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The drug-metabolism activity of adrenocortical cells of normal and 3-methylcholanthrene (MC)-treated ddY mice were examined ultrastructurally and immunohistochemically. Immunoblot analyses performed prior to immunohistochemistry revealed the presence of a protein — possible equivalent to cytochrome P-450 found in the liver microsomes of MC-treated male rats — in the adrenal homogenate of MC-treated female mice. Immunohistochemistry demonstrated the presence of a cytochrome-P-450-like protein in the adrenal-cortical cells (especially in the zona-reticularis cells) of MC-treated female mice; at the same time, a remarkable increase in the amount of SER in these cells was observed by electron microscopy. These findings suggest that cells of the mouse adrenal gland, particularly those of the zona-reticularis, might participate not only in steroid biosynthesis but also in some sort of drug metabolism (detoxication). In addition, there might be a sex-related difference in ddY mice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00953985
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  • 3
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of 17β-hydroxysteroid dehydrogenase in porcine testis (1986)
    Springer
    Histochemistry and cell biology 85 (1986), S. 287-289 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The immunocytochemical localization of 17β-hydroxysteroid dehydrogenase (17β-HSD) in porcine testes was examined by applying an indirect-immunofluorescence method using an antiporcine testicular 17β-HSD antibody. Only the Leydig cells located in the interstitial tissue exhibited a positive immunoreaction for 17β-HSD: the germ cells and Sertoli cells located in the seminiferous tubules were entirely negative. These results suggest that, in porcine testis, the biosynthesis of testicular testosterone, the final step of which is the conversion of androstenedione to testosterone, takes place in the Leydig cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493478
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  • 4
    Electronic Resource
    Electronic Resource
    Light and electron microscopic immunocytochemistry on the localization of 3β-hydroxysteroid dehydrogenase/isomerase in the bovine adrenal cortical cells (1988)
    Springer
    Histochemistry and cell biology 89 (1988), S. 35-39 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization of 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD) was studied in bovine adrenal glands by light as well as electron microscopic immunocytochemistry, using anti-bovine adrenal 3β-HSD antibody. With light microscopy the cytoplasm of the glomerulosa cells was weakly immunostained, while that of the fasciculata-reticularis cells was intensely immunostained though both the capsular connective tissue cells and the medullary cells were entirely negative for this reaction. Electron microscopic immunocytochemistry revealed that the positive reaction products for 3β-HSD were present on the membrane of smooth endoplasmic reticulum of the cortical cells, especially that of the fasciculata and reticularis cells. Other cell organelles such as mitochondria and Golgi apparatus were entirely negative. The present results indicate that 3β-HSD is present in the membrane of smooth endoplasmic reticulum of bovine adrenal cortical cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00496581
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  • 5
    Electronic Resource
    Electronic Resource
    Further immunocytochemical study on the localization of aromatase in the ovary of rats and mice (1989)
    Springer
    Histochemistry and cell biology 90 (1989), S. 413-416 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The precise localization of estrogen biosynthesis in the ovary of rats and mice were immunocytochemically studied using new antisera against aromatase cytochrome P-450. The positive reaction for aromatase was detected mainly on the granulosa cells of large, apparently preovulatory follicles. In addition, the cells of some corpora lutea showed very weak positive reaction but most corpora lutea were negative to the staining. Those cells such as the granulosa cells of smaller follicles, the theca interna cells, the interstitial gland cells, oocytes, peritoneal epithelial cells were entirely negative. These results indicate that in the ovary of rats and mice, the granulosa cells of preovulatory follicles are the main site for synthesis of estrogen from androgen which is provided by the theca interna cell and the interstitial gland cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00494352
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  • 6
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of calspectin (a non-erythroid spectrin-like protein) in thyroid glands of normal and TSH-treated rats (1987)
    Springer
    Histochemistry and cell biology 86 (1987), S. 537-539 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization of calspectin (fodrin, a nonerythroid spectrin-like protein), which is known to bind calmodulin and F-actin, was detected in the thyroid gland of normal and TSH-treated rats by means of light-microscopic immunocytochemistry. Calspectin was demonstrated in the cytoplasm of the follicle epithelial cells especially along the baso-lateral plasma membrane in normal rats. In TSH-treated animals, in addition to the baso-lateral plasma membrane region, the apical plasma membrane region of the follicle epithelial cells also showed positive reaction to the immunostaining. These results suggest that calspectin, in conjugation with calmodulin and actin, play a role in the secretory activities including reabsorption activity of colloid of the follicle epithelial cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00489544
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  • 7
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of aromatase in rat testis (1985)
    Springer
    Histochemistry and cell biology 83 (1985), S. 401-404 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The immunocytochemical localization of armatase in the testes of young and adult rats was investigated by an indirect-immunofluorescent method using antihuman placental aromatase-II cytochrome P-450 antibody. In both young (1 and 2 weeks old) and adult rats, only the Leydig cells in the interstitial tissue showed a positive immunoreaction for aromatase, while the germ cells and Sertoli cells in the seminiferous tubule were entirely negative. In addition, electron microscopy revealed that the Leydig cells in the testes of young as well as adult rats have a well-developed smooth endoplasmic reticulum, mitochondria with tubulovesicular cristae, and a few lipid droplets; these structures being characteristic of steroid secretory cells. On the basis of these results, we suggest that estrogens are mainly synthesized in Leydig cells of the testes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00509199
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  • 8
    Electronic Resource
    Electronic Resource
    Ultrastructural and immunocytochemical studies on the cytoskeleton in the anterior pituitary of rats, with special regard to the relationship between actin filaments and secretory granules (1989)
    Springer
    Cell & tissue research 258 (1989), S. 25-30 
    Details
    ISSN: 1432-0878
    Keywords: Anterior pituitary secretory cells ; Actin filaments ; Secretory granules ; Intracellular transport ; Heavy meromyosin ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary As previously reported, in anterior pituitary cells of the rat, secretory granules are linked with adjacent granules, cytoorganelles, microtubules, and plasma membrane by thin filaments, 4–10 nm in diameter. The quick-freeze, deep-etching method revealed that some of the filaments linking adjacent secretory granules show 5 nm-spaced striations on their surface which are known to be characteristic of actin. Immunocytochemistry showed that actin is localized in the cytoplasm beneath the plasma membrane, and around or between secretory granules. The heavy meromyosin decoration method demonstrated that actin filaments are mainly located in the cytoplasm beneath the plasma membrane, while some actin filaments are connected with the limiting membrane of the secretory granules. The actin filaments associated with the secretory granules are considered to be involved in the intracellular transport of the granules, while those localized in the peripheral cytoplasmic matrix might control the approach of the secretory granules to the plasma membrane and their release.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223140
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  • 9
    Electronic Resource
    Electronic Resource
    Ultrastructural and immunohistochemical studies on non-ciliated cells of the tracheal epithelium of normal, phenobarbital-treated, and 3-methylcholanthrene-treated mice (1985)
    Springer
    Cell & tissue research 240 (1985), S. 501-504 
    Details
    ISSN: 1432-0878
    Keywords: Non-ciliated tracheal cell ; Smooth endoplasmic reticulum ; Ultrastructure ; Cytochrome P-450 ; Immunohistochemistry ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Non-ciliated SER-rich cells of the tracheal epithelium of normal, phenobarbital-treated and 3-methylcholanthrene-treated mice were studied ultrastructurally and immunohistochemically. The apical portion of these cells protrudes into the tracheal lumen, especially in the mice treated with the two compounds, and the apical cytoplasm is filled with numerous tubular elements of SER. Besides, the non-ciliated cells of 3-methylcholanthrene-treated mice show a strong positive reaction to the antiserum against microsomal cytochrome P-450 of liver. These findings support the concept that the non-ciliated tracheal cell may be involved in the metabolism of endogeneous and exogeneous chemical compounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222368
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  • 10
    Electronic Resource
    Electronic Resource
    Ultrastructural and immunohistochemical studies on steroid-secreting cells of the testis and ovary of normal and 3-methylcholanthrene-treated mice (1986)
    Springer
    Cell & tissue research 245 (1986), S. 681-683 
    Details
    ISSN: 1432-0878
    Keywords: Smooth endoplasmic reticulum ; Cytochrome P-450 ; Testis ; Ovary ; Immunohistochemistry ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The testis and ovary of normal and 3-methylcholanthrene-treated mice were studied ultrastructurally and immunohistochemically in order to learn whether steroid-secreting cells of the gonads are involved in drug metabolism. The steroid-secreting cells, i.e., Leydig cells of the testis, and theca interna cells, interstitial gland cells, and corpus luteum cells of the ovary of 3-methylcholanthrene-treated mice, show a strong positive reaction to the antiserum against, hepatic microsomal cytochrome P-450, of liver which is the terminal oxidase of the drug-metabolizing enzyme complex. In addition, it was found that elements of smooth endoplasmic reticulum (SER) in drug-treated mice become well developed as compared with those in control animals. These findings indicate that the steroid secreting cells in testis as well as ovary are involved in the metabolism of both endogenous and exogenous chemical compounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218572
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