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  • 1
    Electronic Resource
    Electronic Resource
    Accumulation of amyloid in pituitary adenomas (1985)
    Springer
    Acta neuropathologica 68 (1985), S. 87-92 
    Details
    ISSN: 1432-0533
    Keywords: Pituitary neoplasm ; Anterior pituitary hormone ; Amyloid ; Electron microscopy ; Bromocriptine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The accumulation of amyloid in pituitary adenomas was examined in relation to the types of adenoma and the effect of bromocriptine treatment. Amyloid had accumulated in 34 of 48 adenomas (71%). The occurrence in prolactin-secreting adenomas and growth hormone-secreting adenomas was 79%, respectively, while that in non-functioning adenomas was 50%. Treatment with bromocriptine enhanced the occurrence and extent of the amyloid accumulation in prolactin- or growth hormone-secreting adenomas. Electron microscopy revealed the initial appearance of the amyloid fibrils in the smooth endoplasmic reticulum and a possible sequential process of their release from the cells. The presence of secretory granules in vesicles containing amyloid fibrils and their simultaneous release with amyloid fibrils suggested that degradation of secretory granules was involved in the formation of amyloid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688628
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  • 2
    Electronic Resource
    Electronic Resource
    S–100 protein-positive cells in hidrocystomas (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 13 (1986), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The histogenesis of hidrocystomas was examined by the use of immunostaining for S–100 protein. In normal sweat glands, S–100 protein was found exclusively in the secretory cells of eccrine glands, whereas this protein was not present in the other parts of eccrine glands or at any levels of the structure of apocrine glands. On the bases of this immunostaining pattern in normal sweat glands, we attempted to correlate the origin of 8 cases of hidrocystoma to the presence of S–100 protein-positive cells. S–100 protein was detected in the cells of one solitary eccrine hidrocystoma, but not in those of 2 cases of “classic”, multiple-lesion type of eccrine hidrocystoma. This indicated that the former arose from the secretory portion of the eccrine gland and the latter from the eccrine ductal cells. Two of the 5 cases of apocrine hidrocystoma showed positive staining in a part of the lining cells of the cyst wall, while the other 3 cases were negative to this protein. This finding suggests that some of the tumors diagnosed morphologically as apocrine hidrocystoma differentiate in the direction of eccrine secretory cells. In addition to S–100 protein, we also surveyed for the presence of carcinoembryonic antigen (CEA), and all cases examined were consistently positive to this substance. The detection of S–100 protein was considered to be more helpful in classifying hidrocystomas than that of CEA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1986.tb01509.x
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  • 3
    Electronic Resource
    Electronic Resource
    Trial use of carbon-fiber-reinforced plastic as a non-Bragg window material of x-ray transmission : INTERNATIONAL CONFERENCE ON SYNCHROTRON RADIATION INSTRUMENTATION (1989)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 60 (1989), S. 2432-2435 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: The carbon-fiber-reinforced plastic (CFRP) that can be used for window material in place of beryllium is a composite-type material comprising twisted carbon fibers and bisphenol-A plastic. Since the constituent elements of hydrogen, carbon, and nitrogen are thoroughly dispersed as a compound, the mass absorption coefficient of CFRP is somewhat larger than that of beryllium. The diffraction pattern of CFRP shows only a single halo pattern without a Bragg-like peak which is found in Mylar. Through x-ray diffraction, CFRP was confirmed to be practical for use as a non-Bragg window material with a moderately low mass-absorption coefficient and could be substituted for crystalline beryllium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1140738
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  • 4
    Electronic Resource
    Electronic Resource
    A study of an in vitro model for invasion of oral squamous cell carcinoma (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 18 (1989), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: An in vitro model for studying the invasion mechanism of oral squamous cell carcinoma (SCC) was developed using a specific culture matrix composed of collagen gel combined with human fibroblasts. Five SCC cell lines cultured on collagen - only gels showed stratified growth on the gels. However, all five cell lines showed invasive growth into the matrix when cultured on the fibroblast -incorporated collagen gels. Moreover, fibroblast-conditioned medium was shown to promote the invasion of HSC-3 cells into the collagen gels. These results suggest that fibroblasts play an important role in the invasion of oral SCC cells in vitro. Four cell lines, which were newly established in our laboratory, were tested in this assay system. These cell lines cultured on fibroblast-incorporated collagen gels expressed morphologic and biologic characteristics in vitro, similar to those in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1989.tb01350.x
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  • 5
    Electronic Resource
    Electronic Resource
    Prognosis of cutaneous angiosarcoma in Japan: A statistical study of sixty-nine cases (1986)
    Springer
    European journal of plastic surgery 8 (1986), S. 151-158 
    Details
    ISSN: 1435-0130
    Keywords: Cutaneous angiosarcoma ; Prognosis ; Statistical study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The prognosis of 69 cases of cutaneous angiosarcoma reported in Japan during 1967–1982 was studied statistically. These cases were followed for a minimum of five years. They included 43 males and 26 females. The mean age was 66.8 years. The lesions were confined to the scalp-face region in 60 cases. There was a history of antecendent trauma at the tumor sites in 14 patients and of irradiation in 2 patients. The sites of metastases in order of frequency were the lung, liver and regional lymph nodes. Sixty-four patients died as a result of their tumor. Mean survival was 13.4 months. Five-year survival rate was 6.1%. Only two patients had long-term, disease-free survival. Duration between the onset and the initial visit, and survival period were not correlative. Tumor size was shown to be an important prognostic factor in the scalpface cases. No type of treatment has been particularly effective.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00263349
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  • 6
    Electronic Resource
    Electronic Resource
    NaCl-aided Hoechst 33258 staining method for DNA quantification and its application (1989)
    Springer
    Histochemistry and cell biology 92 (1989), S. 65-68 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We investigated the effect of salt on the fluorescence staining procedure for quantification of the amount of DNA in cell nuclei in situ. For this, NaCl was added at various concentrations to the Hoechst 33258 fluorochrome (Hoe) medium for staining DNA. The fluorescence intensity of free DNA-Hoe solution was not changed by the addition of NaCl, but that of the nuclei-Hoe complex in situ increased 4-fold on increasing the NaCl concentration up to 1 M. SDS polyacrylamide gel electrophoresis showed that histones H1, H2A, and H2B dissociated from cell nuclei in the presence of 1 M NaCl, resulting in increasing accessibility of DNA to the fluorochrome. The applicability of the NaCl-aided fluorescence staining method was evaluated by measuring the ploidy classes of various cells. The amount of DNA in spermatozoa is half that in 2n hepatocytes, but by the conventional Hoe staining procedure the fluorescence intensity of spermatozoa is higher than that of 2n hepatocytes, due to differences in accessibility of the dye to DNA. In contrast, by the NaCl-aided procedure, the fluorescence intensity of 2n hepatocytes was twice that of spermatozoa. The effectiveness of the NaCl-aided Hoe staining method was checked using cultivated human gingival cells and hepatocytes of LEC rats with hereditary hepatitis. In all cases, reasonable proportionality between the fluorescence intensity and the amount of DNA was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495018
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  • 7
    Electronic Resource
    Electronic Resource
    Acute capsular infarction (1985)
    Springer
    Neuroradiology 27 (1985), S. 248-253 
    Details
    ISSN: 1432-1920
    Keywords: Lacunar syndrome ; capsular infarction ; transient ischemic attack ; reversible ischemic neurological deficit ; cerebral infarction ; internal capsule ; basal ganglia ; CT-scan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Sixty-three patients with lacunar-type of acute capsular infarction were treated in our service during the last 2 years. Their lesions were identified by computed tomography (CT) and classified into six types according to their locations: anterior, lateral, posterior, superior, inferior and multiple. The lesions were thought to be in the watershed areas of the regional arterial supplies, and the areas were considered to be prone to ischemia. The clinical course of each type showed characteristic features of ischemic strokes. In the majority of the patients with the lateral type, reversible ischemic neurological deficit (RIND) was seen as the predominant symptom, transient ischemic attack (TIA) was noted in the patients with the superior type, and major completed stroke was observed in those with posterior type.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00344496
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  • 8
    Electronic Resource
    Electronic Resource
    Characterization and estimation of ito (indium-tin-oxide) by Mössbauer spectrometry (1988)
    Springer
    Hyperfine interactions 42 (1988), S. 1207-1210 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract ITO films and powders, prepared by several methods, were characterized by conversion electron and transmission Mössbauer spectrometry (CEMS and TMS). The slight change of FWHM of Sn(IV), observed in ITO films, was attributed to the presence of the acid-insoluble segregated SnO2 and the acid-soluble Sn(IV) that is incorporated in the In2O3 lattice. A good correlation was found between the area intensities of a doublet and a singlet and the ratios of acid-soluble Sn(IV) to acid-insoluble Sn(IV). The peaks of acid-soluble Sn(IV) in ITO were deconvoluted to two doublets corresponding to different sites. The results suggested that Sn(IV) atoms were preferentially substituted to the centers of trigonally distorted octahedra (site b) rather than to the centers of more distorted octahedra (site d).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02395608
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  • 9
    Electronic Resource
    Electronic Resource
    Spatio-temporal organization of intracellular ATP content and oscillation patterns in response to blue light byPhysarum polycephalum (1986)
    Springer
    Protoplasma 135 (1986), S. 31-37 
    Details
    ISSN: 1615-6102
    Keywords: Blue light ; Intracellular ATP ; Oscillation pattern ; Physarum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Phototactic responses in a giant amoeboid cell ofPhysarum plasmodium were studied both by analyzing intracellular ATP content and by applying image processing for recording oscillatory changes in thickness with use of a microcomputer. The ATP content fluctuated and deviated from the initial level upon exposure to light with varying wavelength from 400 to 600 nm. The maximum decrease in the integrated value $$\int\limits_{\text{O}}^{\text{T}} {{\text{ATP}}}$$ dt with T=9 minutes occurred at the wavelength 450 nm. The ATP in a migrating plasmodium distributed twice as high in the front as in the rear. This polar pattern became unstable, and a new wavy pattern appeared by stimulating a local frontal part with blue light. In a concentrically extending plasmodium, peripheral and inside regions oscillated in opposite phase to each other. When part of this organism was exposed to light, stimulated and unslimulated regions began to oscillate in opposite phase, and phase waves propagated inward the stimulated region. And the protoplasm there became thinner, the strongest avoidance reaction occurring to 450 nm light as in ATP response. Phototactic behavior inPhysarum is discussed in terms of bifurcation in spatio-temporal organization appearing in a self-organizing system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01277050
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  • 10
    Electronic Resource
    Electronic Resource
    Fundamental conditions in pressure-programmed supercritical fluid chromatography-mass spectrometry and some applications to vitamin analysis (1986)
    Springer
    Chromatographia 21 (1986), S. 617-621 
    Details
    ISSN: 1612-1112
    Keywords: Supercritical fluid chromatography-mass spectrometry ; Pressure programming ; Analysis of vitamins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A combined system of pressure-programmed packed capillary supercritical fluid chromatography-mass spectrometry (SFC/MS) was constructed using a self-soupting interface assisted by vacuum nebulizing. For the optimum operation of the SFC/MS system, fundamental analytical conditions such as the flow rate of the mobile phase, the pump pressure and the composition of the mobile phase were examined. The use of large packing materials indicated that the capacity factor for a sample solute is almost constant under a given pump pressure regardless of the flow rate of the supercritical fluid. The SFC/MS system was applied to the analysis of both water- and fat-soluble vitamins. Both types vitamins were clearly separated under basically the same SFC conditions. High quality mass spectra of the vitamins were obtained; selected ion monitoring (SIM) traces of the vitamins are also reported as well as their UV traces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02311914
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