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  • 1
    Electronic Resource
    Electronic Resource
    Bradyrhizobium japonicum mutants defective in root-nodule bacteroid development and nitrogen fixation (1986)
    Springer
    Archives of microbiology 144 (1986), S. 355-366 
    Details
    ISSN: 1432-072X
    Keywords: Bradyrhizobium ; Electron microscopy ; Mutants ; Nitrogen fixation ; Nodulation ; Soybean ; Symbiosis ; Transposon Tn5
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genome of the slow-growing Bradyrhizobium japonicum (strain 110) was mutagenized with transposon Tn5. A total of 1623 kanamycin/streptomycin resistant derivatives were screened in soybean infection tests for nodulation (Nod) and symbiotic nitrogen fixation (Fix). In this report we describe 14 strains possessing a stable, reproducible Nod+Fix- phenotype. These strains were also grown under microaerobic culture conditions to test them for free-living nitrogen fixation activity (Nif). In addition to strains having reduced Fix and Nif activities, there were also strains that had reduced symbiotic Fix activity but were Nif+ ex planta. Analysis of the genomic structure revealed that the majority of the strains had a single Tn5 insertion without any further apparent physical alteration. A few strains had additional insertions (by Tn5 or IS50), or a deletion, or had cointegrated part of the vector used for Tn5 mutagenesis. One of the insertions was found in a known nif gene (nifD) whereas all other mutations seem to affect different, hitherto unknown genes or operons. Several mutant strains had an altered nodulation phenotype, inducing numerous, small, widely distributed nodules. Light and electron microscopy revealed that most of these mutants were defective in different stages of bacteroid development and/or bacteroid persistence. The protein patterns of the mutants were inspected by two-dimensional gel electrophoresis after labelling microaerobic cultures with l-(35S)methionine. Of particular interest were mutants lacking a group of proteins the synthesis of which was known to be under oxygen control. Such strains can be regarded as potential regulatory mutants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409885
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  • 2
    Electronic Resource
    Electronic Resource
    Authors' reply (1989)
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 96 (1989), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.1989.tb03371.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Secular trend in the rate of small-for-gestational-age infants: Haguenau Study 1971-1985 (1988)
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 95 (1988), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary. Between 1971 and 1985, at the Haguenau Maternity Hospital, a 20% decrease was observed in the rate of small-for-gestationalage infants (birthweight 〈10th centile using figures from all the study births) among 20 101 births between 28 and 42 weeks. This decrease could not be explained by simultaneous changes in socio-demographic and anthropometric characteristics of the pregnant women, nor a recruitment bias. The earliness of prenatal care at the Maternity Hospital may have played a role in the downward trend. Further research is needed to confirm the influence of prenatal care on the rate of small-forgestational-age babies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.1988.tb06815.x
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  • 4
    Electronic Resource
    Electronic Resource
    Zum enzymatisch-immunochemischen Nachweis des Haschischkonsums und seiner dünnschichtchromatographischen Absicherung (1985)
    Springer
    International journal of legal medicine 94 (1985), S. 219-225 
    Details
    ISSN: 1437-1596
    Keywords: Cannabinoids, enzyme immunoassay ; THC-metabolite, detection in urine and blood ; Cannabinoide, Enzymimmunoassay ; THC-Metabolit, Nachweis in Urin und Blut
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Mit Hilfe des Enzymimmunoassay (EMIT-dau-Syva) lassen sich Cannabinoide in Blut und Urin nach inhalativer (10 mg THC) oder oraler (30 mg THC) Aufnahme nachweisen. Die Kurvenverläufe werden mitgeteilt. Im Urin waren erhöhte Spiegel bis zu einer Woche nachweisbar. Eine Absicherung der EMIT-Befunde ist mittels Dünnschichtchromatographie nach absorptiver Anreicherung an einer C18-Extraktionssäule möglich. Nachweisgrenze ist 10 ng THC-Carbonsäure/ml bei Einsatz von 10 ml Urin.
    Notes: Summary The EMIT cannabinoid assay was used for screening blood and urine after smoking tetrahydrocannabinol (THC; 10 mg) or ingestion of THC (30 mg). Cannabinoid levels in urine remain detectable up to 1 week. Confirmation was done by adsorption of the THC carboxylic acid onto a C18 extraction column and elution with acetone and TLC. The method is simple and sensitive and is applicable with common laboratory equipment. The detection limit is 10 ng/ml, using 10 ml urine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00204086
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    Paper (German National Licenses)
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